##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71383
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.831976801199165	32.0	32.0	32.0	14.0	32.0
2	31.225137637812924	32.0	32.0	32.0	32.0	32.0
3	31.341846097810404	32.0	32.0	32.0	32.0	32.0
4	31.506549178375803	32.0	32.0	32.0	32.0	32.0
5	31.30645952117451	32.0	32.0	32.0	32.0	32.0
6	34.701021251558494	36.0	36.0	36.0	32.0	36.0
7	34.78562122634241	36.0	36.0	36.0	32.0	36.0
8	34.81345698555678	36.0	36.0	36.0	32.0	36.0
9	34.812476359917625	36.0	36.0	36.0	32.0	36.0
10	34.696944650687136	36.0	36.0	36.0	32.0	36.0
11	34.888292730762224	36.0	36.0	36.0	32.0	36.0
12	34.79747278763851	36.0	36.0	36.0	32.0	36.0
13	34.778784864743706	36.0	36.0	36.0	32.0	36.0
14	34.70246417214182	36.0	36.0	36.0	32.0	36.0
15	34.71281677710379	36.0	36.0	36.0	32.0	36.0
16	34.64522365269042	36.0	36.0	36.0	32.0	36.0
17	34.62032977039351	36.0	36.0	36.0	32.0	36.0
18	34.49451550088957	36.0	36.0	36.0	32.0	36.0
19	34.47563145286693	36.0	36.0	36.0	32.0	36.0
20	34.39129764789936	36.0	36.0	36.0	32.0	36.0
21	34.274112884020006	36.0	36.0	36.0	32.0	36.0
22	34.283176666713366	36.0	36.0	36.0	32.0	36.0
23	34.23333286636874	36.0	36.0	36.0	32.0	36.0
24	34.27663449280641	36.0	36.0	36.0	32.0	36.0
25	34.18634688931538	36.0	36.0	36.0	32.0	36.0
26	34.0870095120687	36.0	36.0	36.0	32.0	36.0
27	34.06664051664962	36.0	36.0	36.0	32.0	36.0
28	33.92953504335766	36.0	36.0	36.0	32.0	36.0
29	33.825770841797066	36.0	36.0	36.0	32.0	36.0
30	33.7159827970245	36.0	36.0	36.0	32.0	36.0
31	33.59997478391214	36.0	36.0	36.0	27.0	36.0
32	33.44542818318087	36.0	36.0	36.0	21.0	36.0
33	33.20430634744967	36.0	36.0	36.0	14.0	36.0
34	32.993738004847096	36.0	36.0	36.0	14.0	36.0
35	32.713993527870784	36.0	36.0	36.0	14.0	36.0
36	32.57047196111119	36.0	36.0	36.0	14.0	36.0
37	32.42969614614124	36.0	36.0	36.0	14.0	36.0
38	32.031800288584115	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	3.0
21	5.0
22	21.0
23	46.0
24	92.0
25	222.0
26	487.0
27	882.0
28	1560.0
29	2435.0
30	3433.0
31	4757.0
32	6129.0
33	8607.0
34	15503.0
35	27200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.95090855597741	17.973576922538072	15.352284349300193	14.723230172184318
2	11.9664345852654	26.796296036871524	39.26425059187762	21.97301878598546
3	19.060560637686844	38.179958813723154	27.6718546432624	15.087625905327599
4	9.837076054522784	18.920471260664304	32.68425255312889	38.55820013168401
5	8.660325287533446	44.36210302172786	28.897636692209627	18.079934998529062
6	24.49322667862096	36.09823067116821	21.32300407660087	18.085538573609963
7	22.20587823260766	37.251688100641616	22.74242806309714	17.80000560365358
8	32.911658401277634	25.761396430472665	21.624499173461096	19.702445994788604
9	31.41224100976423	13.353319417788548	21.136685205160894	34.09775436728633
10	18.655702338091704	29.917487356933727	29.041928750542844	22.384881554431725
11	29.86396178040545	26.804152598175886	21.246339857377023	22.08554576404164
12	25.514478237115277	31.069022036059007	29.093761820041188	14.322737906784527
13	33.316055643500555	23.930067382990348	28.214000532339632	14.539876441169467
14	26.970006864379474	20.608548253785916	33.05548940223863	19.365955479595982
15	24.206043455724753	24.735581300869956	37.353431489290166	13.704943754115126
16	17.902021489710435	28.156563887760388	33.53179328411527	20.409621338413906
17	16.047238137932002	26.31018589860331	34.7085440511046	22.934031912360087
18	18.522617429920288	22.300827928218204	41.64156731995013	17.53498732191138
19	18.301276214224675	28.281243433310454	40.23086729333315	13.186613059131727
20	21.617191768348206	18.864435509855287	44.36490480926831	15.153467912528193
21	23.610663603378956	22.718294271745375	36.274743286216605	17.396298838659064
22	20.64357059804155	24.17942647409047	37.08866256671757	18.088340361150415
23	17.30664163736464	25.625148844963086	38.02866228653881	19.039547231133465
24	16.104674782511243	29.797010492694337	38.35226874746088	15.746045977333539
25	19.948727288009753	24.64872588711598	37.35203059551994	18.050516229354326
26	15.722230783239707	25.59432918201813	40.56848269195747	18.114957342784695
27	18.725747026602974	28.12014064973453	39.4393623131558	13.714750010506705
28	16.75749127943628	25.067593124413374	40.634324699158064	17.54059089699228
29	14.455822814955944	28.37090063460488	42.60258044632476	14.570696104114425
30	17.588221285179944	26.891556813246854	41.121835731196505	14.398386170376702
31	14.37737276382332	28.61745793816455	38.9490494935769	18.05611980443523
32	13.434571256461622	29.865654287435383	39.53602398330134	17.16375047280165
33	14.62112827984254	29.417368280963252	38.561001919224466	17.40050151996974
34	13.905271563257356	30.60812798565485	39.93107602650491	15.555524424582885
35	16.893377975148145	29.93429808217643	38.030063180309035	15.142260762366389
36	16.365241023773166	31.663000994634576	37.23715730636145	14.734600675230796
37	16.48571788801255	32.83414818654302	37.367440426992424	13.312693498452013
38	15.492484204922741	33.1311376658308	35.4524186430943	15.923959486152164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	589.0
1	389.0
2	189.0
3	189.0
4	643.5
5	1098.0
6	1098.0
7	1246.0
8	1394.0
9	1309.5
10	1225.0
11	1225.0
12	1510.5
13	1796.0
14	2048.5
15	2301.0
16	2301.0
17	3245.0
18	4189.0
19	4189.0
20	4316.5
21	4444.0
22	3452.0
23	2460.0
24	2460.0
25	1788.5
26	1117.0
27	1117.0
28	1577.5
29	2038.0
30	2544.5
31	3051.0
32	3051.0
33	2566.0
34	2081.0
35	2081.0
36	2002.0
37	1923.0
38	2202.0
39	2481.0
40	2481.0
41	3067.0
42	3653.0
43	4436.0
44	5219.0
45	5219.0
46	8047.5
47	10876.0
48	10876.0
49	9128.5
50	7381.0
51	5188.0
52	2995.0
53	2995.0
54	2332.0
55	1669.0
56	1669.0
57	1630.5
58	1592.0
59	1492.0
60	1392.0
61	1392.0
62	1288.0
63	1184.0
64	1060.5
65	937.0
66	937.0
67	838.5
68	740.0
69	740.0
70	647.0
71	554.0
72	470.5
73	387.0
74	387.0
75	286.5
76	186.0
77	186.0
78	156.0
79	126.0
80	94.5
81	63.0
82	63.0
83	48.0
84	33.0
85	33.0
86	21.0
87	9.0
88	6.0
89	3.0
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008405362621352424
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0014008937702254037
8	0.0014008937702254037
9	0.0
10	0.0
11	0.008405362621352424
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	71383.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.65509995377051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45688345638888	32.301808553857356
2	10.622814144911153	8.424975134135579
3	3.0593139506129226	3.6395220150455994
4	1.2576394531387995	1.994872728800975
5	0.7277351891758223	1.4429205833321659
6	0.42392341117038185	1.0086435145622907
7	0.349736814215565	0.9708193827662048
8	0.21902709577136395	0.6948433100318002
9	0.23669057123679657	0.8447389434459185
>10	1.2929664040696647	9.92112968073631
>50	0.14484049881654715	4.069596402504798
>100	0.16956936446815277	13.916478713419162
>500	0.01766347546543258	4.603336928960677
>1k	0.021196170558519096	16.16631410840116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3533	4.949357690206352	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2643	3.7025622347057427	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1563	2.189596962862306	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1309	1.8337699452250535	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1299	1.8197610075227997	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1193	1.6712662678789065	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	992	1.3896866200636004	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	633	0.8867657565526808	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	596	0.8349326870543408	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	551	0.7718924673941975	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	514	0.7200593978958576	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	491	0.6878388411806733	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	489	0.6850370536402225	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	416	0.582771808413768	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	415	0.5813709146435425	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	398	0.5575557205497107	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	379	0.5309387389154281	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	370	0.5183306949833993	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.516929801213174	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	313	0.43847975008055134	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	304	0.42587170614852277	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	281	0.39365114943333845	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	270	0.378241317960859	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	260	0.364232380258605	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	235	0.3292100360029699	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	213	0.298390373058011	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	207	0.2899850104366586	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	206	0.2885841166664332	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	194	0.27177339142372836	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	185	0.25916534749169967	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	184	0.25776445372147433	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	183	0.2563635599512489	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.2521608786405727	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	167	0.23394925962764243	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	167	0.23394925962764243	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	159	0.2227421094658392	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	158	0.2213412156956138	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	151	0.21153495930403599	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	146	0.20453049045290897	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	145	0.20312959668268354	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	142	0.19892691537200732	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	140	0.19612512783155653	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	134	0.18771976521020411	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	133	0.18631887143997872	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	130	0.1821161901293025	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	129	0.1807152963590771	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	129	0.1807152963590771	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	126	0.17651261504840088	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	122	0.17090903996749926	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	118	0.16530546488659764	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	116	0.16250367734614685	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	116	0.16250367734614685	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	114	0.15970188980569602	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	114	0.15970188980569602	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	111	0.15549920849501983	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	108	0.1512965271843436	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	108	0.1512965271843436	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	107	0.1498956334141182	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	102	0.1428911645629912	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	99	0.13868848325231498	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	98	0.13728758948208958	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	98	0.13728758948208958	No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA	98	0.13728758948208958	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	94	0.13168401440118796	No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA	93	0.13028312063096256	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	91	0.12748133309051174	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	85	0.11907597046915933	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA	82	0.1148732891584831	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	82	0.1148732891584831	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	82	0.1148732891584831	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTA	80	0.11207150161803231	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG	78	0.1092697140775815	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	78	0.1092697140775815	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	78	0.1092697140775815	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.10646792653713069	No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA	75	0.10506703276690528	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	72	0.10086435145622909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.001400893770225404	0.0
17	0.0	0.0	0.0	0.001400893770225404	0.0
18	0.0	0.0	0.0	0.001400893770225404	0.0
19	0.0	0.0	0.0	0.002801787540450808	0.0
20	0.0	0.0	0.0	0.002801787540450808	0.0
21	0.0	0.0	0.0	0.002801787540450808	0.0
22	0.0	0.0	0.0	0.014008937702254039	0.0
23	0.0	0.0	0.0	0.032220556715184284	0.0
24	0.0	0.0	0.0	0.05463485703879075	0.0
25	0.0	0.0	0.0	0.05743664457924156	0.0
26	0.0	0.0	0.0	0.07985094490284801	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGGAT	20	0.0037301576	32.000004	23
TGGGTTT	40	0.004443102	20.000002	32
CCACTGC	620	0.0	13.419354	22
CACTGCT	600	0.0	13.333332	23
ACCACTG	640	0.0	13.250001	21
AAGTACT	85	0.0015827069	13.176471	2
AAAGTAC	85	0.0015827069	13.176471	1
GATACCA	660	0.0	13.090909	18
ACTGCTT	600	0.0	13.066666	24
TACCACT	655	0.0	12.946565	20
TGGAAGC	450	0.0	12.800001	6
CTGCTTC	575	0.0	12.8	25
ATACCAC	675	0.0	12.8	19
TGCTTCC	555	0.0	12.684685	26
GCTTCCA	420	0.0	12.571428	27
ATGGAAG	460	0.0	12.521739	5
CTTCCAT	410	0.0	12.487804	28
TTGGGTT	90	0.0024325645	12.444445	31
TGATACC	695	0.0	12.431655	17
TTGATAC	715	0.0	12.083916	16
>>END_MODULE