FastQCFastQC Report
Thu 2 Feb 2017
SRR4062905_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062905_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141384
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29400.2575820232279408No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24280.212724201495728No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT23180.2030867788579479No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20550.18004457746034638No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20410.1788179963973562No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19160.16786637976351518No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18880.1654132176375348No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18810.1647999271060397No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18810.1647999271060397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18370.16094495805092765No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18270.16006882872022038No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18230.15971837698793745No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16980.14876676035409644No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16120.1412320481100138No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15600.13667617559033593No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15550.1362381109249823No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15490.13571243332655794No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15330.13431062639742627No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14660.12844055988168748No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14570.12765204348405096No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13810.1209934605706756No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13120.11494816818879536No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13020.11407203885808809No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT12720.11144365086596622No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC12350.10820197234234928No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12270.10750106887778346No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12110.10609926194865181No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11730.10276997049196415No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC11700.10250713169275197No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC11700.10250713169275197No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG11680.1023319058266105No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG11510.10084248596440812No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC11500.10075487303133739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA5950.019.10012
CAAGACG5700.018.5231974
ATACCGT3650.018.4070516
AAGACGG5850.018.3208985
TCTAGCG4900.018.28423728
GCGCAAG6300.017.5416091
ATCGTTT3750.017.49268729
TAACCCG1650.017.45313528
CTAGCGG5100.017.25426529
GCTCGAC652.1002773E-417.23088611
CTCGTGT652.1009808E-417.2301338
TACCGTC3900.017.2293787
ACCCGTT1800.016.88826430
CTATAGA1252.8157956E-916.65691
TATACAG1252.8612703E-916.6364695
CGGTCCA5450.016.4404810
AGCGGCG5550.016.43182631
TAAACGC4400.016.36231428
GACGGAC6400.016.2486867
CCGTCGT4050.016.1976419
Produced by FastQC (version 0.11.5)