##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062905_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141384
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220105591106936	32.0	32.0	32.0	32.0	32.0
2	30.79803729507335	32.0	32.0	32.0	32.0	32.0
3	30.843136928500837	32.0	32.0	32.0	32.0	32.0
4	30.919375950600323	32.0	32.0	32.0	32.0	32.0
5	30.80016891773496	32.0	32.0	32.0	32.0	32.0
6	34.47052876157367	36.0	36.0	36.0	32.0	36.0
7	34.38848187814092	36.0	36.0	36.0	32.0	36.0
8	34.37239439137048	36.0	36.0	36.0	32.0	36.0
9	34.52946773390901	36.0	36.0	36.0	32.0	36.0
10	34.21537712110911	36.0	36.0	36.0	32.0	36.0
11	34.51317523287518	36.0	36.0	36.0	32.0	36.0
12	34.31182056170404	36.0	36.0	36.0	32.0	36.0
13	34.41547454669068	36.0	36.0	36.0	32.0	36.0
14	34.30532931949283	36.0	36.0	36.0	32.0	36.0
15	34.22146359156953	36.0	36.0	36.0	32.0	36.0
16	34.244825580172844	36.0	36.0	36.0	32.0	36.0
17	34.15071351972693	36.0	36.0	36.0	32.0	36.0
18	34.1885798294001	36.0	36.0	36.0	32.0	36.0
19	34.17526003518535	36.0	36.0	36.0	32.0	36.0
20	34.16644442185978	36.0	36.0	36.0	32.0	36.0
21	34.145845745165516	36.0	36.0	36.0	32.0	36.0
22	34.11809522474469	36.0	36.0	36.0	32.0	36.0
23	34.04845783715209	36.0	36.0	36.0	32.0	36.0
24	34.03015987608027	36.0	36.0	36.0	32.0	36.0
25	34.00911963020333	36.0	36.0	36.0	32.0	36.0
26	33.952329803116214	36.0	36.0	36.0	32.0	36.0
27	33.96120061258963	36.0	36.0	36.0	32.0	36.0
28	33.95172877839535	36.0	36.0	36.0	32.0	36.0
29	33.901552851625745	36.0	36.0	36.0	32.0	36.0
30	33.87408356872008	36.0	36.0	36.0	32.0	36.0
31	33.90015192082595	36.0	36.0	36.0	32.0	36.0
32	33.84543589186461	36.0	36.0	36.0	32.0	36.0
33	33.80835809858908	36.0	36.0	36.0	32.0	36.0
34	33.81905651384635	36.0	36.0	36.0	32.0	36.0
35	33.78083887631156	36.0	36.0	36.0	32.0	36.0
36	33.73052276884905	36.0	36.0	36.0	27.0	36.0
37	33.73020648616066	36.0	36.0	36.0	27.0	36.0
38	33.1004114303337	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	4.0
11	11.0
12	4.0
13	3.0
14	138.0
15	274.0
16	383.0
17	486.0
18	596.0
19	791.0
20	1083.0
21	1615.0
22	2415.0
23	3658.0
24	5539.0
25	8260.0
26	11897.0
27	17169.0
28	23891.0
29	31970.0
30	42553.0
31	56758.0
32	77875.0
33	116133.0
34	252099.0
35	485769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.44026911572502	16.844381288211714	11.067428050139032	25.64792154592423
2	17.48069320383243	19.044258803110125	36.44325423609954	27.0317937569579
3	19.358787428455265	23.055893393524958	27.985689300447582	29.59962987757219
4	12.524422918203596	14.509272385411611	35.13982613510714	37.826478561277646
5	15.112057334104328	36.07571536210552	32.81264784734269	15.999579456447458
6	35.638118451452144	34.8610759440992	16.051986237700184	13.448819366748468
7	31.3722638481002	29.71033412068156	20.13695653697616	18.78044549424208
8	29.358918646134867	31.70747093002881	18.883478303533256	20.050132120303072
9	27.577684265491953	13.574146452154972	18.207560477070363	40.64060880528271
10	16.681473304245735	25.566682437049888	30.065184250643956	27.68666000806042
11	39.19656061848589	20.55796202451795	21.10546291818239	19.140014438813775
12	25.4877497450419	23.256384466305228	27.480453212121635	23.775412576531238
13	29.825698974101943	18.16666725082454	25.27145816319735	26.736175611876163
14	24.257941055863398	19.391492389273836	23.877141885805813	32.47342466905695
15	25.80238085222732	26.430632089625483	21.702228718854723	26.064758339292478
16	26.888051808995073	24.997261819956556	22.922203266714508	25.192483104333864
17	24.658410566312867	25.400653383996296	24.266520379691816	25.674415669999018
18	26.252354708979876	23.64635994848116	25.511114226385008	24.59017111615396
19	26.348660605152553	24.160364637137157	24.602930520398246	24.888044237312045
20	26.372593257836286	23.3189494666287	24.229732985784068	26.078724289750948
21	28.28215300826058	23.334040114814023	23.432084140874984	24.95172273605041
22	26.8602039931202	23.180757601113804	24.380691361615963	25.57834704415003
23	24.86863445549343	22.953505753622462	25.152000252348017	27.025859538536086
24	25.462046146782235	24.48283778526289	24.42018597510752	25.634930092847352
25	25.678190813513517	23.69764054966798	24.457280857484854	26.16688777933365
26	25.247114370138767	25.016299032924046	25.20207295657447	24.534513640362714
27	26.180537666629867	23.760231735333857	24.170026864055263	25.889203733981013
28	24.99007728763424	23.780163512280943	25.440345003728137	25.78941419635668
29	24.793037943627812	24.172677503697628	25.30159330992086	25.732691242753695
30	24.666449381262286	24.673370972772737	25.462257174799095	25.197922471165878
31	25.77344246954858	24.463212088395426	23.75824260553076	26.005102836525236
32	25.42148786398835	24.061826981882287	23.602093727054957	26.91459142707441
33	24.80259214994936	23.584060871363334	24.803468349905195	26.80987862878212
34	26.21356671199652	23.929783117666027	24.73371630052293	25.122933869814524
35	26.764871855985195	23.619805540756527	25.059059809886996	24.556262793371282
36	24.88015008512827	24.855352040230667	24.17493925355256	26.089558621088504
37	26.512285264166913	24.34508089996723	24.260089057431706	24.882544778434152
38	25.136728367478188	23.945207015773047	24.826033852208944	26.09203076453982
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	93.0
2	117.0
3	117.0
4	327.0
5	537.0
6	537.0
7	690.5
8	844.0
9	830.5
10	817.0
11	817.0
12	1071.0
13	1325.0
14	1708.5
15	2092.0
16	2092.0
17	3133.5
18	4175.0
19	4175.0
20	5060.0
21	5945.0
22	6152.5
23	6360.0
24	6360.0
25	7604.0
26	8848.0
27	8848.0
28	11355.0
29	13862.0
30	16930.5
31	19999.0
32	19999.0
33	27160.0
34	34321.0
35	34321.0
36	38184.0
37	42047.0
38	49413.5
39	56780.0
40	56780.0
41	59825.0
42	62870.0
43	73944.5
44	85019.0
45	85019.0
46	86405.5
47	87792.0
48	87792.0
49	95419.5
50	103047.0
51	106100.5
52	109154.0
53	109154.0
54	104357.5
55	99561.0
56	99561.0
57	98624.0
58	97687.0
59	88573.0
60	79459.0
61	79459.0
62	77621.5
63	75784.0
64	63592.0
65	51400.0
66	51400.0
67	43363.0
68	35326.0
69	35326.0
70	28508.5
71	21691.0
72	16874.0
73	12057.0
74	12057.0
75	8920.5
76	5784.0
77	5784.0
78	5828.5
79	5873.0
80	4646.5
81	3420.0
82	3420.0
83	3527.0
84	3634.0
85	3634.0
86	2420.5
87	1207.0
88	1048.5
89	890.0
90	890.0
91	654.5
92	419.0
93	362.0
94	305.0
95	305.0
96	274.5
97	244.0
98	244.0
99	433.5
100	623.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11862791137776593
2	0.05204208224401253
3	0.012265810629901943
4	0.0033292914566876706
5	3.504517322829127E-4
6	4.380646653536409E-4
7	0.0
8	0.0
9	8.761293307072817E-5
10	3.504517322829127E-4
11	0.0014018069291316508
12	0.0017522586614145636
13	0.012265810629901943
14	0.008761293307072817
15	0.024531621259803887
16	0.01007548730313374
17	0.021728007401540586
18	0.004731098385819321
19	0.007447099311011895
20	0.005169163051172963
21	0.00525677598424369
22	0.006220518248021701
23	0.009024132106285002
24	0.013667617559033594
25	0.01629600555115544
26	0.018573941810994372
27	0.00718426051179971
28	0.005519614783455875
29	0.009812648503921556
30	0.002453162125980389
31	0.0050815501181022335
32	0.006132905314950972
33	0.00806038984250699
34	0.01051355196848738
35	0.014718972755882331
36	0.014456133956670147
37	0.00858606744093136
38	0.006132905314950972
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1141384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.70168712885363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23147049616456	35.60138284484313
2	13.654008001458362	12.753304194779446
3	4.51490695466349	6.325613160377388
4	1.9552504406490332	3.6525397735097735
5	1.0065963996169254	2.350487505997008
6	0.6007754679073536	1.6834336762139923
7	0.3752692215006464	1.226799404012816
8	0.26732616815050353	0.9987666453056104
9	0.18966856374587837	0.7972057730015136
>10	0.9617263068180547	8.127712487197078
>50	0.10122760932043462	3.3024950746521085
>100	0.11398283847301968	11.458014702775946
>500	0.01952918431955503	6.017310231502569
>1k	0.008262347212119435	5.704934525831621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2940	0.2575820232279408	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2428	0.212724201495728	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2318	0.2030867788579479	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2055	0.18004457746034638	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2041	0.1788179963973562	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1916	0.16786637976351518	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1888	0.1654132176375348	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1881	0.1647999271060397	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1881	0.1647999271060397	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1837	0.16094495805092765	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1827	0.16006882872022038	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1823	0.15971837698793745	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1698	0.14876676035409644	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1612	0.1412320481100138	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1560	0.13667617559033593	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1555	0.1362381109249823	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1549	0.13571243332655794	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1533	0.13431062639742627	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1466	0.12844055988168748	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1457	0.12765204348405096	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1381	0.1209934605706756	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1312	0.11494816818879536	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1302	0.11407203885808809	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1272	0.11144365086596622	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1235	0.10820197234234928	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1227	0.10750106887778346	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1211	0.10609926194865181	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1173	0.10276997049196415	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1170	0.10250713169275197	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1170	0.10250713169275197	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1168	0.1023319058266105	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1151	0.10084248596440812	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1150	0.10075487303133739	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.761293307072817E-5	0.0
8	0.0	0.0	0.0	8.761293307072817E-5	0.0
9	0.0	0.0	0.0	1.7522586614145634E-4	0.0
10	0.0	0.0	0.0	1.7522586614145634E-4	0.0
11	0.0	0.0	0.0	1.7522586614145634E-4	0.0
12	0.0	0.0	0.0	2.628387992121845E-4	7.009034645658254E-4
13	0.0	0.0	0.0	2.628387992121845E-4	7.885163976365535E-4
14	0.0	0.0	0.0	2.628387992121845E-4	7.885163976365535E-4
15	0.0	0.0	0.0	2.628387992121845E-4	7.885163976365535E-4
16	0.0	0.0	0.0	2.628387992121845E-4	7.885163976365535E-4
17	0.0	0.0	0.0	4.3806466535364083E-4	7.885163976365535E-4
18	0.0	0.0	0.0	6.132905314950972E-4	7.885163976365535E-4
19	0.0	0.0	0.0	6.132905314950972E-4	7.885163976365535E-4
20	0.0	0.0	0.0	7.009034645658254E-4	8.761293307072817E-4
21	0.0	0.0	0.0	7.885163976365535E-4	8.761293307072817E-4
22	0.0	0.0	0.0	0.0011389681299194662	8.761293307072817E-4
23	0.0	0.0	0.0	0.0017522586614145633	8.761293307072817E-4
24	0.0	0.0	0.0	0.0022779362598389324	8.761293307072817E-4
25	0.0	0.0	0.0	0.0028036138582633015	8.761293307072817E-4
26	0.0	0.0	0.0	0.003679743188970583	9.637422637780098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAGA	595	0.0	19.1001	2
CAAGACG	570	0.0	18.523197	4
ATACCGT	365	0.0	18.407051	6
AAGACGG	585	0.0	18.320898	5
TCTAGCG	490	0.0	18.284237	28
GCGCAAG	630	0.0	17.541609	1
ATCGTTT	375	0.0	17.492687	29
TAACCCG	165	0.0	17.453135	28
CTAGCGG	510	0.0	17.254265	29
GCTCGAC	65	2.1002773E-4	17.230886	11
CTCGTGT	65	2.1009808E-4	17.230133	8
TACCGTC	390	0.0	17.229378	7
ACCCGTT	180	0.0	16.888264	30
CTATAGA	125	2.8157956E-9	16.6569	1
TATACAG	125	2.8612703E-9	16.636469	5
CGGTCCA	545	0.0	16.44048	10
AGCGGCG	555	0.0	16.431826	31
TAAACGC	440	0.0	16.362314	28
GACGGAC	640	0.0	16.248686	7
CCGTCGT	405	0.0	16.197641	9
>>END_MODULE