FastQCFastQC Report
Thu 2 Feb 2017
SRR4062905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141384
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31070.27221338305075243No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC25930.22718033545239816No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24360.21342510496029382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22200.19450071141701655No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21030.18424999824774135No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20850.18267296545246822No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20340.1782047058658611No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20220.17715335066901236No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20010.17531347907452707No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18830.16497515297218115No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18410.16129540978321058No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17930.1570899889958156No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17230.15095708368086463No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16730.14657643702732823No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16650.1458755335627624No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16130.14131966104308455No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15750.13799036958639688No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15530.13606288505884084No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15120.132470754802941No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14930.13080610907459717No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14860.13019281854310205No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14430.12642546242106076No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14070.12327139683051454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13520.1184526855116245No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13440.11775178204705865No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13430.11766416911398793No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13040.11424726472422954No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12930.11328352246045151No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC12810.11223216726360277No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT12550.10995423100376386No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC12530.1097790051376224No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG12510.10960377927148093No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT12500.10951616633841021No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG12490.10942855340533948No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT11760.10303280929117632No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG11640.10198145409432759No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC11610.10171861529511542No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG11420.10005396956677157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT3800.021.0532956
TACCGTC3900.020.5134667
CGTCGAA400.00448378119.99975422
ACCGTCG4050.019.3586358
TAGGACG1208.185452E-1118.6672534
CGTCGTA4150.018.50579610
CCGTCGT4300.018.2331339
CTAGCGG5150.018.01919629
TAGACTG1251.4733814E-1017.9205655
GTACCGT904.365229E-717.7783376
TAGGACA904.365229E-717.7783374
ATCGTTT3700.017.72951129
TCTAGCG5250.017.67597428
GTCGTAG4450.017.25821311
TTAGGAC1302.582965E-1017.2313123
CGGTCCA5800.016.8273810
CATCGTT4000.016.79979328
TACCGTG1151.8399078E-816.6961787
TCAGATA4650.016.5159262
TCGTTTA4150.016.19257230
Produced by FastQC (version 0.11.5)