##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062905_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1141384
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18740669222628	32.0	32.0	32.0	32.0	32.0
2	31.27042695534544	32.0	32.0	32.0	32.0	32.0
3	31.36872078108682	32.0	32.0	32.0	32.0	32.0
4	31.469060368815402	32.0	32.0	32.0	32.0	32.0
5	31.37277112698268	32.0	32.0	32.0	32.0	32.0
6	34.91498479039482	36.0	36.0	36.0	36.0	36.0
7	34.937813216235725	36.0	36.0	36.0	36.0	36.0
8	34.87765730026003	36.0	36.0	36.0	32.0	36.0
9	34.9973094068254	36.0	36.0	36.0	36.0	36.0
10	34.82483896742902	36.0	36.0	36.0	32.0	36.0
11	35.01416350676021	36.0	36.0	36.0	36.0	36.0
12	34.89275476088678	36.0	36.0	36.0	32.0	36.0
13	34.96084402795203	36.0	36.0	36.0	36.0	36.0
14	34.89399798840706	36.0	36.0	36.0	32.0	36.0
15	34.86726027349253	36.0	36.0	36.0	32.0	36.0
16	34.873096170964374	36.0	36.0	36.0	32.0	36.0
17	34.83102444050381	36.0	36.0	36.0	32.0	36.0
18	34.83829543781935	36.0	36.0	36.0	32.0	36.0
19	34.83303165280046	36.0	36.0	36.0	32.0	36.0
20	34.81927729843768	36.0	36.0	36.0	32.0	36.0
21	34.80550279310031	36.0	36.0	36.0	32.0	36.0
22	34.796532981012525	36.0	36.0	36.0	32.0	36.0
23	34.72291270948252	36.0	36.0	36.0	32.0	36.0
24	34.69640278819398	36.0	36.0	36.0	32.0	36.0
25	34.667221548576116	36.0	36.0	36.0	32.0	36.0
26	34.6139581420451	36.0	36.0	36.0	32.0	36.0
27	34.600645356865	36.0	36.0	36.0	32.0	36.0
28	34.57297631647193	36.0	36.0	36.0	32.0	36.0
29	34.521020094902326	36.0	36.0	36.0	32.0	36.0
30	34.49864813244272	36.0	36.0	36.0	32.0	36.0
31	34.50778002845668	36.0	36.0	36.0	32.0	36.0
32	34.47745018328626	36.0	36.0	36.0	32.0	36.0
33	34.431873935502864	36.0	36.0	36.0	32.0	36.0
34	34.429891254827474	36.0	36.0	36.0	32.0	36.0
35	34.38918540999348	36.0	36.0	36.0	32.0	36.0
36	34.359835077414786	36.0	36.0	36.0	32.0	36.0
37	34.359880636139984	36.0	36.0	36.0	32.0	36.0
38	33.89289056093304	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	19.0
22	98.0
23	286.0
24	759.0
25	1979.0
26	4005.0
27	7876.0
28	13574.0
29	21323.0
30	32254.0
31	46738.0
32	67083.0
33	108954.0
34	264847.0
35	571581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.712180290378264	18.17291263241827	11.681460127752416	26.43344694945105
2	16.37538286851752	20.066077674121942	35.757816825888575	27.80072263147197
3	18.318112046427846	23.820729920868	28.274620986451538	29.58653704625262
4	12.09935665766881	15.250621395272226	35.302348116276484	37.34767383078248
5	14.614888591394307	36.50086211126142	32.62985988939743	16.254389407946842
6	35.66988469251776	35.27045698069798	15.658635182055452	13.401023144728807
7	31.047059823353724	29.93927510366864	20.17615564357789	18.837509429399752
8	29.17047424877099	32.04817398145901	18.708596651547317	20.072755118222688
9	27.38194431667144	13.48310373619942	18.122446547807108	41.01250539932203
10	16.321643913388602	25.777790627787212	30.22444877840217	27.67611668042202
11	39.32938182041206	20.50195604270438	20.97648806003496	19.1921740768486
12	25.20293434520873	23.384519953494852	27.455647948665607	23.95689775263081
13	29.78488340002155	18.175438350559542	25.215418865392014	26.824259384026895
14	24.11230576212738	19.4424488165245	23.79085391069088	32.65439151065724
15	25.75198180454606	26.591664155095916	21.561455215773133	26.094898824584888
16	26.994333195488984	24.948045530689058	22.715930834846116	25.34169043897584
17	24.620723612736818	25.53312469773538	24.170130297954064	25.676021391573737
18	26.183037435254043	23.610721720297466	25.508768302341718	24.697472542106777
19	26.35984033419077	24.194223854548515	24.483872211280342	24.962063599980375
20	26.426776615056806	23.230569203703574	24.175387073938307	26.167267107301313
21	28.210488319443762	23.406320747443456	23.43216656269932	24.951024370413464
22	26.669026374997372	23.31204923145935	24.437437356752856	25.581487036790424
23	24.778339279331057	22.788299117562538	25.28132512809011	27.152036475016295
24	25.410203752637152	24.46836472212682	24.399062892067874	25.722368633168152
25	25.562387417381004	23.655316703230465	24.61634296608328	26.16595291330525
26	25.234452208897267	24.907918807342668	25.235240725294904	24.62238825846516
27	26.408751488326004	23.61599918695127	24.116006634057104	25.859242690665624
28	24.93648062352372	23.61738030321084	25.605755819251012	25.840383254014426
29	24.769139921358633	23.985880299706324	25.410729430235573	25.834250348699474
30	24.66680801553202	24.432706258367034	25.65972538602258	25.24076034007836
31	25.69897598003827	24.296468147442052	23.83772682988372	26.166829042635957
32	25.44743924919221	23.954514869667	23.779026164726332	26.81901971641446
33	24.784822636378294	23.468963994589025	24.91843235931115	26.82778100972153
34	26.156578329466683	23.81249430515935	24.882861508484435	25.148065856889527
35	26.73473607480042	23.488589291596867	25.03986388454718	24.736810749055532
36	24.96635663370084	24.742856041437413	24.298220406103468	25.992566918758282
37	26.572914987418784	24.212534957560294	24.262299103544468	24.952250951476454
38	25.208803705679866	23.705890135037933	24.873771556085906	26.211534603196295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	35.5
2	49.0
3	49.0
4	203.5
5	358.0
6	358.0
7	521.0
8	684.0
9	782.5
10	881.0
11	881.0
12	1084.5
13	1288.0
14	1715.5
15	2143.0
16	2143.0
17	3289.0
18	4435.0
19	4435.0
20	5120.0
21	5805.0
22	6104.5
23	6404.0
24	6404.0
25	7513.0
26	8622.0
27	8622.0
28	11263.0
29	13904.0
30	16949.5
31	19995.0
32	19995.0
33	27520.0
34	35045.0
35	35045.0
36	38453.0
37	41861.0
38	49374.5
39	56888.0
40	56888.0
41	60371.5
42	63855.0
43	74594.0
44	85333.0
45	85333.0
46	86529.0
47	87725.0
48	87725.0
49	95347.5
50	102970.0
51	105954.5
52	108939.0
53	108939.0
54	104548.5
55	100158.0
56	100158.0
57	99166.5
58	98175.0
59	89227.5
60	80280.0
61	80280.0
62	77977.5
63	75675.0
64	63463.5
65	51252.0
66	51252.0
67	43446.5
68	35641.0
69	35641.0
70	28791.0
71	21941.0
72	16866.5
73	11792.0
74	11792.0
75	8639.0
76	5486.0
77	5486.0
78	5660.0
79	5834.0
80	4584.0
81	3334.0
82	3334.0
83	3191.0
84	3048.0
85	3048.0
86	1942.5
87	837.0
88	696.0
89	555.0
90	555.0
91	324.0
92	93.0
93	67.5
94	42.0
95	42.0
96	29.0
97	16.0
98	16.0
99	17.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00998787437006301
2	0.0
3	0.0
4	8.761293307072817E-5
5	0.0
6	8.761293307072817E-5
7	6.132905314950972E-4
8	0.0018398715944852914
9	0.0032416785236169423
10	8.761293307072818E-4
11	0.00429303372046568
12	4.380646653536409E-4
13	2.628387992121845E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	8.761293307072817E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.761293307072817E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1141384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.201754493947114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28437170315813	32.83499560760107
2	14.591717135194205	12.899589969099512
3	4.907403243291403	6.507475000882994
4	2.160710711963683	3.8202881769064154
5	1.1315668630618665	2.500862033727325
6	0.6640073734528373	1.7610174542119785
7	0.4224666970496523	1.307163845740022
8	0.292133319280344	1.0330244206665304
9	0.22228085840138925	0.8842683538585827
>10	1.0618383808285936	8.500239064996878
>50	0.10778881546135842	3.4146956951697414
>100	0.12488092714252404	12.25790622678348
>500	0.019090077824523967	5.792701619509341
>1k	0.009743893889600775	6.485772530846172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3107	0.27221338305075243	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2593	0.22718033545239816	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2436	0.21342510496029382	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2220	0.19450071141701655	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2103	0.18424999824774135	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2085	0.18267296545246822	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2034	0.1782047058658611	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2022	0.17715335066901236	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2001	0.17531347907452707	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1883	0.16497515297218115	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1841	0.16129540978321058	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1793	0.1570899889958156	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1723	0.15095708368086463	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1673	0.14657643702732823	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1665	0.1458755335627624	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1613	0.14131966104308455	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1575	0.13799036958639688	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1553	0.13606288505884084	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1512	0.132470754802941	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1493	0.13080610907459717	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1486	0.13019281854310205	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1443	0.12642546242106076	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1407	0.12327139683051454	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1352	0.1184526855116245	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1344	0.11775178204705865	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1343	0.11766416911398793	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1304	0.11424726472422954	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1293	0.11328352246045151	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1281	0.11223216726360277	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1255	0.10995423100376386	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1253	0.1097790051376224	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1251	0.10960377927148093	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1250	0.10951616633841021	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1249	0.10942855340533948	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1176	0.10303280929117632	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1164	0.10198145409432759	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1161	0.10171861529511542	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	1142	0.10005396956677157	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.761293307072817E-5
2	0.0	0.0	0.0	0.0	8.761293307072817E-5
3	0.0	0.0	0.0	0.0	8.761293307072817E-5
4	0.0	0.0	0.0	0.0	8.761293307072817E-5
5	0.0	0.0	0.0	0.0	8.761293307072817E-5
6	0.0	0.0	0.0	0.0	8.761293307072817E-5
7	0.0	0.0	0.0	0.0	8.761293307072817E-5
8	0.0	0.0	0.0	0.0	8.761293307072817E-5
9	0.0	0.0	0.0	1.7522586614145634E-4	8.761293307072817E-5
10	0.0	0.0	0.0	1.7522586614145634E-4	1.7522586614145634E-4
11	0.0	0.0	0.0	1.7522586614145634E-4	1.7522586614145634E-4
12	0.0	0.0	0.0	1.7522586614145634E-4	7.009034645658254E-4
13	0.0	0.0	0.0	1.7522586614145634E-4	7.885163976365535E-4
14	0.0	0.0	0.0	1.7522586614145634E-4	7.885163976365535E-4
15	0.0	0.0	0.0	1.7522586614145634E-4	8.761293307072817E-4
16	0.0	0.0	0.0	1.7522586614145634E-4	8.761293307072817E-4
17	0.0	0.0	0.0	1.7522586614145634E-4	8.761293307072817E-4
18	0.0	0.0	0.0	2.628387992121845E-4	8.761293307072817E-4
19	0.0	0.0	0.0	2.628387992121845E-4	9.637422637780098E-4
20	0.0	0.0	0.0	2.628387992121845E-4	0.0011389681299194662
21	0.0	0.0	0.0	3.504517322829127E-4	0.0012265810629901945
22	0.0	0.0	0.0	7.009034645658254E-4	0.0013141939960609225
23	0.0	0.0	0.0	0.0012265810629901945	0.0013141939960609225
24	0.0	0.0	0.0	0.0017522586614145633	0.0013141939960609225
25	0.0	0.0	0.0	0.002190323326768204	0.0013141939960609225
26	0.0	0.0	0.0	0.0029788397244047576	0.0013141939960609225
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGT	380	0.0	21.053295	6
TACCGTC	390	0.0	20.513466	7
CGTCGAA	40	0.004483781	19.999754	22
ACCGTCG	405	0.0	19.358635	8
TAGGACG	120	8.185452E-11	18.667253	4
CGTCGTA	415	0.0	18.505796	10
CCGTCGT	430	0.0	18.233133	9
CTAGCGG	515	0.0	18.019196	29
TAGACTG	125	1.4733814E-10	17.920565	5
GTACCGT	90	4.365229E-7	17.778337	6
TAGGACA	90	4.365229E-7	17.778337	4
ATCGTTT	370	0.0	17.729511	29
TCTAGCG	525	0.0	17.675974	28
GTCGTAG	445	0.0	17.258213	11
TTAGGAC	130	2.582965E-10	17.231312	3
CGGTCCA	580	0.0	16.82738	10
CATCGTT	400	0.0	16.799793	28
TACCGTG	115	1.8399078E-8	16.696178	7
TCAGATA	465	0.0	16.515926	2
TCGTTTA	415	0.0	16.192572	30
>>END_MODULE