FastQCFastQC Report
Thu 2 Feb 2017
SRR4062904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252516
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54642.1638232824850703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39411.5606931837982543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22030.8724199654675348No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA19080.7555956850258994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.6918373489204644No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA13510.5350156029716928No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA10320.408686974290738No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA7440.29463479541890414No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6310.2498851557921082No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5860.23206450284338417No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.22810435774366775No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA5630.22295616911403635No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4310.17068225379777915No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA3100.12276449809121007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2940.11642826593166374No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2790.11048804828208907No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.10692391769234424No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2550.10098370004276956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTACA200.003755406731.9871274
CGGGCTC308.3891785E-426.67178332
TCCCTTT350.002062177322.85720
ACGAGAC350.002064554322.8524743
GTCTCAC502.7653256E-522.422071
ATATAAG453.5028238E-421.3543531
GTGTATA450.00878170517.7952941
GAACTGA450.00883200417.77766822
ACCGATA450.00883200417.77766812
TCTGTCG450.00883200417.7776688
TCAGCAT450.00884209117.7741473
CGTCCCT450.00884209117.77414718
ACTGGAG450.00884209117.7741473
GTACAGC450.008852188517.7706286
CCACACC450.008852188517.7706284
GCCTTAG550.001351696717.4717431
GGTAGGG550.001362516317.450982
CCTTAGG652.095504E-417.2272512
GGATAAG805.546092E-516.0157641
ATCACCC600.00244137715.9935645
Produced by FastQC (version 0.11.5)