##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062904_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252516
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.723530390153496	32.0	32.0	32.0	32.0	32.0
2	30.501722663118375	32.0	32.0	32.0	32.0	32.0
3	30.647024346972074	32.0	32.0	32.0	32.0	32.0
4	30.58112357236769	32.0	32.0	32.0	32.0	32.0
5	30.70640276259722	32.0	32.0	32.0	32.0	32.0
6	34.24916044923886	36.0	36.0	36.0	32.0	36.0
7	34.00410271032331	36.0	36.0	36.0	32.0	36.0
8	33.902259658793895	36.0	36.0	36.0	32.0	36.0
9	33.87550887864531	36.0	36.0	36.0	32.0	36.0
10	33.81491073832945	36.0	36.0	36.0	32.0	36.0
11	34.18603177620428	36.0	36.0	36.0	32.0	36.0
12	34.033859240602574	36.0	36.0	36.0	32.0	36.0
13	34.095875112864135	36.0	36.0	36.0	32.0	36.0
14	34.007191623501086	36.0	36.0	36.0	32.0	36.0
15	34.05260260735953	36.0	36.0	36.0	32.0	36.0
16	34.00268101823251	36.0	36.0	36.0	32.0	36.0
17	33.914286619461734	36.0	36.0	36.0	32.0	36.0
18	34.00682729015191	36.0	36.0	36.0	32.0	36.0
19	33.936114939251375	36.0	36.0	36.0	32.0	36.0
20	33.74571512300211	36.0	36.0	36.0	32.0	36.0
21	33.71539625211868	36.0	36.0	36.0	27.0	36.0
22	33.77802990701579	36.0	36.0	36.0	32.0	36.0
23	33.834085760902276	36.0	36.0	36.0	32.0	36.0
24	33.871960588635964	36.0	36.0	36.0	32.0	36.0
25	33.87908884981546	36.0	36.0	36.0	32.0	36.0
26	33.88929018359233	36.0	36.0	36.0	32.0	36.0
27	33.9357110044512	36.0	36.0	36.0	32.0	36.0
28	33.933909138430835	36.0	36.0	36.0	32.0	36.0
29	33.866824280441634	36.0	36.0	36.0	32.0	36.0
30	33.84327725767872	36.0	36.0	36.0	32.0	36.0
31	33.82828414833119	36.0	36.0	36.0	32.0	36.0
32	33.753084953032676	36.0	36.0	36.0	27.0	36.0
33	33.59764925786881	36.0	36.0	36.0	27.0	36.0
34	33.511904196169745	36.0	36.0	36.0	21.0	36.0
35	33.50058214132966	36.0	36.0	36.0	21.0	36.0
36	33.46032726639104	36.0	36.0	36.0	21.0	36.0
37	33.46186776283483	36.0	36.0	36.0	21.0	36.0
38	32.80808740832264	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	0.0
10	0.0
11	5.0
12	2.0
13	0.0
14	121.0
15	227.0
16	269.0
17	357.0
18	477.0
19	672.0
20	783.0
21	934.0
22	1121.0
23	1462.0
24	1800.0
25	2392.0
26	3101.0
27	3979.0
28	5334.0
29	6963.0
30	9253.0
31	12136.0
32	16160.0
33	23960.0
34	51759.0
35	109247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.72113078127788	18.073072576968062	12.735562912594414	20.470233729159645
2	16.87138928732754	20.48784740107935	38.80114433340994	23.83961897818317
3	18.96214383599623	28.478861859459286	28.48480287703678	24.074191427507703
4	11.621994107024047	15.436983176504135	34.88934828755188	38.05167442891994
5	13.225300575013069	39.50323939869157	29.5153574426967	17.75610258359866
6	29.77803298813932	37.79537849236679	17.449260439973862	14.977328079520028
7	28.319505771934338	33.80868463259608	18.799675266815832	19.072134328653743
8	31.883254460131084	28.938874918321684	17.212838841256957	21.965031780290282
9	30.323347127893392	12.103835415717878	19.135496901174186	38.43732055521454
10	19.06349746944724	26.411604901114394	26.935140229848642	27.589757399589725
11	36.23485709533446	24.1462748157506	20.185655278383912	19.433212810531025
12	26.895675005643366	26.63984254026589	26.414900062968055	20.04958239112269
13	31.474447582194305	21.556077487018825	25.429240220056165	21.540234710730708
14	26.0136874539593	19.067779828431565	27.416889114196774	27.501643603412358
15	26.19593676596992	25.8968783049921	25.930943242269045	21.97624168676894
16	23.102118086909684	27.726026963230517	24.7342490059091	24.4376059439507
17	21.404070251043706	26.159165986707123	25.740495749922758	26.696268012326414
18	21.945172711503275	25.539995722806157	29.64927010479125	22.86556146089932
19	24.789405106554877	23.6591036004103	30.259129739127676	21.292361553907142
20	27.652058201519196	21.37369208956761	29.071848935850014	21.90240077306318
21	29.331997354444965	20.860280634774792	28.28288428865065	21.524837722129593
22	26.97159196670086	22.396128301498226	28.851995453447337	21.78028427835358
23	25.239807678598304	23.353030171013963	29.641892480613404	21.76526966977433
24	24.032794676806084	24.571451204055766	29.21855196451204	22.17720215462611
25	24.2413200358051	24.39143212477919	29.346715357377672	22.02053248203804
26	23.622140806020397	25.01950688186949	29.574017229428655	21.78433508268145
27	22.86302699063348	25.475751995089013	29.849303946612803	21.81191706766471
28	21.956261732580337	25.60296552106518	29.820753895018655	22.62001885133583
29	21.88064383257818	25.922024016982988	29.50912518614746	22.68820696429137
30	21.801066113280477	26.832629719689827	29.023864779450786	22.342439387578906
31	22.44642553039814	27.405653091695413	27.49832672604644	22.64959465186001
32	22.105917670635016	27.519821939183675	27.223978011707022	23.150282378474284
33	22.39621699973861	26.276247732655307	27.99824157023026	23.32929369737582
34	23.165853426697506	26.173125059408765	27.939545641773073	22.721475872120656
35	22.96977234200979	26.69951363254701	27.85166584813295	22.479048177310247
36	22.487543666479194	28.102993480723377	27.0043012967261	22.405161556071324
37	22.790730832141882	29.06219182313965	26.215776278946347	21.93130106577212
38	21.795074890495915	29.187095547758034	27.223942780651246	21.793886781094805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	122.5
2	157.0
3	157.0
4	325.0
5	493.0
6	493.0
7	805.5
8	1118.0
9	1284.5
10	1451.0
11	1451.0
12	1878.0
13	2305.0
14	3032.0
15	3759.0
16	3759.0
17	5166.0
18	6573.0
19	6573.0
20	7097.5
21	7622.0
22	6100.5
23	4579.0
24	4579.0
25	4014.5
26	3450.0
27	3450.0
28	4432.0
29	5414.0
30	7033.5
31	8653.0
32	8653.0
33	8875.0
34	9097.0
35	9097.0
36	9502.0
37	9907.0
38	10993.5
39	12080.0
40	12080.0
41	12778.5
42	13477.0
43	14792.0
44	16107.0
45	16107.0
46	16485.5
47	16864.0
48	16864.0
49	16945.0
50	17026.0
51	16825.0
52	16624.0
53	16624.0
54	16639.0
55	16654.0
56	16654.0
57	16641.0
58	16628.0
59	15684.5
60	14741.0
61	14741.0
62	13779.0
63	12817.0
64	11687.0
65	10557.0
66	10557.0
67	9541.5
68	8526.0
69	8526.0
70	7373.5
71	6221.0
72	5252.0
73	4283.0
74	4283.0
75	3287.0
76	2291.0
77	2291.0
78	1765.0
79	1239.0
80	986.5
81	734.0
82	734.0
83	545.5
84	357.0
85	357.0
86	244.0
87	131.0
88	98.5
89	66.0
90	66.0
91	50.5
92	35.0
93	35.5
94	36.0
95	36.0
96	34.0
97	32.0
98	32.0
99	178.0
100	324.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11920036750146526
2	0.05623406041597364
3	0.013464493339035943
4	0.004752174119659744
5	0.0
6	3.9601450997164535E-4
7	3.9601450997164535E-4
8	3.9601450997164535E-4
9	3.9601450997164535E-4
10	7.920290199432907E-4
11	0.001980072549858227
12	0.0027721015698015175
13	0.013860507849007588
14	0.008712319219376197
15	0.022572827068383785
16	0.009504348239319489
17	0.019800725498582267
18	0.005544203139603035
19	0.006732246669517971
20	0.005544203139603035
21	0.006732246669517971
22	0.006732246669517971
23	0.008712319219376197
24	0.014256522358979234
25	0.015048551378922522
26	0.01623659490883746
27	0.008316304709404553
28	0.005544203139603035
29	0.01108840627920607
30	0.003960145099716454
31	0.006732246669517971
32	0.007128261179489617
33	0.007128261179489617
34	0.01108840627920607
35	0.012672464319092651
36	0.013464493339035943
37	0.009108333729347844
38	0.005544203139603035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	252516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.62811069397583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.70667354262921	23.595336533130574
2	14.722628582253702	11.079693959986695
3	7.2544913015565635	8.189184051703654
4	4.300283107233442	6.472461150976572
5	2.755296420640517	5.183829935528838
6	1.8891356283612406	4.26507627239462
7	1.3092393992653946	3.448494352833088
8	1.0292894955639518	3.098417526018153
9	0.7514444783565046	2.544789241077793
>10	3.134175989559763	18.983351550000794
>50	0.09577233547680941	2.3990559014082273
>100	0.03894040013892251	2.524592501069239
>500	0.005262216234989528	1.2276449809121006
>1k	0.006314659481987434	4.824248760474584
>5k	0.0010524432469979055	2.1638232824850703
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5464	2.1638232824850703	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3941	1.5606931837982543	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2203	0.8724199654675348	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1908	0.7555956850258994	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1747	0.6918373489204644	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1351	0.5350156029716928	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1032	0.408686974290738	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	744	0.29463479541890414	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	631	0.2498851557921082	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	586	0.23206450284338417	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	576	0.22810435774366775	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	563	0.22295616911403635	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	431	0.17068225379777915	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	310	0.12276449809121007	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	294	0.11642826593166374	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	279	0.11048804828208907	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	270	0.10692391769234424	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	255	0.10098370004276956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	7.920290199432907E-4	0.0
22	0.0	0.0	0.0	0.001188043529914936	0.0
23	0.0	0.0	0.0	0.0027721015698015175	0.0
24	0.0	0.0	3.9601450997164535E-4	0.005148188629631389	0.0
25	0.0	0.0	3.9601450997164535E-4	0.006336232159546326	0.0
26	0.0	0.0	3.9601450997164535E-4	0.006336232159546326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTACA	20	0.0037554067	31.987127	4
CGGGCTC	30	8.3891785E-4	26.671783	32
TCCCTTT	35	0.0020621773	22.857	20
ACGAGAC	35	0.0020645543	22.852474	3
GTCTCAC	50	2.7653256E-5	22.42207	1
ATATAAG	45	3.5028238E-4	21.354353	1
GTGTATA	45	0.008781705	17.795294	1
GAACTGA	45	0.008832004	17.777668	22
ACCGATA	45	0.008832004	17.777668	12
TCTGTCG	45	0.008832004	17.777668	8
TCAGCAT	45	0.008842091	17.774147	3
CGTCCCT	45	0.008842091	17.774147	18
ACTGGAG	45	0.008842091	17.774147	3
GTACAGC	45	0.0088521885	17.770628	6
CCACACC	45	0.0088521885	17.770628	4
GCCTTAG	55	0.0013516967	17.471743	1
GGTAGGG	55	0.0013625163	17.45098	2
CCTTAGG	65	2.095504E-4	17.227251	2
GGATAAG	80	5.546092E-5	16.015764	1
ATCACCC	60	0.002441377	15.993564	5
>>END_MODULE