FastQCFastQC Report
Thu 2 Feb 2017
SRR4062904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252516
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62372.469942498693152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT50972.0184859573254763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29681.1753710655958434No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21570.854203298008839No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA17960.7112420599090751No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA12960.5132348049232524No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA12810.5072945872736777No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA10710.42413154017963217No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7210.2855264616895563No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA6620.26216160560122925No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.25146921383199483No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA5160.204343487145369No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA3810.15088152829919688No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3670.14533732515959386No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3320.13147681731058625No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2920.11563623691172044No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.11365616436186222No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA2770.10969601926214577No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2740.10850797573223082No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2720.10771594671228754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCC200.003745403632.0045554
TACTGGT302.2372165E-532.0045557
CATAGTA308.391614E-426.6704642
TTTCGTC308.4013294E-426.66518417
TATGGGT350.002060398522.8603974
GTACTGG350.002060398522.8603976
TAGGACT350.002060398522.8603974
TCCCAAG350.002060398522.8603972
ACCTAGG400.004465154420.006811
CCCTTAC400.004465154420.006811
ATACTGC400.00447027820.0028486
GGACCGT400.00447027820.0028486
TAGGACC400.00447027820.0028484
ATTGAGC400.00447027820.0028488
CTTAGGG400.00447027820.0028483
GCTCCGG400.004475406419.99888621
GGTACTG507.167809E-419.2027345
AGATCTT450.008824456517.7803085
GTAGAAC450.008824456517.7803083
AGTAGCT450.008824456517.7803087
Produced by FastQC (version 0.11.5)