##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062904_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252516
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.696862773052004	32.0	32.0	32.0	32.0	32.0
2	31.302578846488935	32.0	32.0	32.0	32.0	32.0
3	31.386518082022526	32.0	32.0	32.0	32.0	32.0
4	31.509009330101854	32.0	32.0	32.0	32.0	32.0
5	31.325959543157662	32.0	32.0	32.0	32.0	32.0
6	34.82697334030319	36.0	36.0	36.0	32.0	36.0
7	34.91655578260387	36.0	36.0	36.0	36.0	36.0
8	34.87553659966101	36.0	36.0	36.0	32.0	36.0
9	34.93788908425605	36.0	36.0	36.0	32.0	36.0
10	34.79346259246939	36.0	36.0	36.0	32.0	36.0
11	35.000320771753074	36.0	36.0	36.0	36.0	36.0
12	34.91850813413804	36.0	36.0	36.0	32.0	36.0
13	34.9440985917724	36.0	36.0	36.0	36.0	36.0
14	34.849803576803055	36.0	36.0	36.0	32.0	36.0
15	34.85851589602243	36.0	36.0	36.0	32.0	36.0
16	34.83995865608516	36.0	36.0	36.0	32.0	36.0
17	34.79526049834466	36.0	36.0	36.0	32.0	36.0
18	34.77565381995596	36.0	36.0	36.0	32.0	36.0
19	34.73487620586418	36.0	36.0	36.0	32.0	36.0
20	34.68375073262684	36.0	36.0	36.0	32.0	36.0
21	34.67386225031285	36.0	36.0	36.0	32.0	36.0
22	34.69748451583266	36.0	36.0	36.0	32.0	36.0
23	34.69840326949579	36.0	36.0	36.0	32.0	36.0
24	34.68864547197009	36.0	36.0	36.0	32.0	36.0
25	34.6885385480524	36.0	36.0	36.0	32.0	36.0
26	34.650679560899114	36.0	36.0	36.0	32.0	36.0
27	34.65800186918849	36.0	36.0	36.0	32.0	36.0
28	34.626760284496825	36.0	36.0	36.0	32.0	36.0
29	34.573159720572164	36.0	36.0	36.0	32.0	36.0
30	34.564019705682014	36.0	36.0	36.0	32.0	36.0
31	34.51022905479257	36.0	36.0	36.0	32.0	36.0
32	34.409391880118484	36.0	36.0	36.0	32.0	36.0
33	34.29734353466711	36.0	36.0	36.0	32.0	36.0
34	34.251920670373366	36.0	36.0	36.0	32.0	36.0
35	34.229268640402985	36.0	36.0	36.0	32.0	36.0
36	34.221962172694006	36.0	36.0	36.0	32.0	36.0
37	34.16006906493054	36.0	36.0	36.0	32.0	36.0
38	33.82109648497521	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	5.0
22	16.0
23	55.0
24	145.0
25	458.0
26	920.0
27	1869.0
28	3359.0
29	5219.0
30	7839.0
31	11237.0
32	15629.0
33	24041.0
34	55679.0
35	126042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.06592048668045	19.323843698264458	13.438764921619416	21.171470893435675
2	16.320549984951448	21.306768680004435	37.59524148964818	24.777439845395936
3	18.61426602670722	28.22237006763928	29.106274453895992	24.057089451757513
4	11.101078743525163	15.945128229498328	34.727304408433525	38.22648861854298
5	12.666920115953047	39.71550317603637	29.699504189833515	17.918072518177066
6	30.33589950735795	37.77740816423514	16.70072391452423	15.185968413882684
7	27.792408371779892	33.67918737500742	18.809971684850403	19.718432568362275
8	31.998463421105782	28.867881936881457	17.257998724797925	21.875655917214832
9	29.724485067858968	11.914426812509653	19.251591032398846	39.10949708723253
10	18.86588466382062	26.907815012236945	26.929199965150445	27.297100358791987
11	36.48687717575119	23.752965358278647	20.141861947968113	19.618295518002054
12	26.250217807980487	27.116301541288472	26.537328327709925	20.096152323021116
13	31.716927707264915	21.203096845731935	25.809555867968236	21.270419579034908
14	25.59877393907713	19.097007714362654	27.75111280077302	27.553105545787197
15	26.219328676202696	26.028449682396364	25.658572130082845	22.093649511318095
16	23.035067223729282	27.2514504088866	24.962081460507296	24.751400906876817
17	21.406564336517288	25.767080105815076	26.500498978282565	26.32585657938507
18	21.999398057944845	24.367564827575283	30.6075654611985	23.025471653281375
19	24.80040868697429	23.2052622408085	31.008332145289803	20.985996926927402
20	27.24896640212897	20.928574822981513	30.07056978567695	21.751888989212564
21	28.814015745536913	20.521867921240634	29.35853569674793	21.30558063647452
22	26.205072153843716	22.19859335646058	29.958497679354974	21.63783681034073
23	24.74259056851843	22.71341221942372	30.85151039934103	21.69248681271682
24	23.775523135167674	23.796907918706143	30.11809152687355	22.30947741925264
25	23.816312629694753	23.749386177509543	30.41431038033234	22.01999081246337
26	23.256744127104817	24.120451773352976	30.823393369133044	21.799410730409164
27	22.684899174705762	24.36954490012514	30.85547054444075	22.09008538072835
28	22.11463828034659	24.20044670436725	30.98338323116159	22.70153178412457
29	21.746740800582934	24.897432241917343	30.444803497600155	22.91102345989957
30	21.922967257520316	25.641939520664035	30.139080295902044	22.296012925913605
31	22.536393733466394	25.912021416464697	29.03142771151135	22.520157138557558
32	22.23898683647769	25.876380110567247	28.74510922080185	23.139523832153213
33	22.50629663070855	25.013068478829066	29.393384973625437	23.087249916836953
34	22.948248823836906	25.045541668646738	29.263096199844764	22.743113307671592
35	22.753013670420884	25.51719495002297	29.170428804511396	22.55936257504475
36	22.59104373584248	26.586038112436437	28.434237830474107	22.38868032124697
37	22.648465839788372	27.559045763436774	27.83982005100667	21.95266834576819
38	21.645757100540163	27.528948660678925	28.94232444676773	21.882969792013178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	33.0
2	35.0
3	35.0
4	163.0
5	291.0
6	291.0
7	556.0
8	821.0
9	1102.5
10	1384.0
11	1384.0
12	1882.0
13	2380.0
14	3154.5
15	3929.0
16	3929.0
17	5844.5
18	7760.0
19	7760.0
20	7869.0
21	7978.0
22	6486.0
23	4994.0
24	4994.0
25	4026.5
26	3059.0
27	3059.0
28	4074.5
29	5090.0
30	6719.5
31	8349.0
32	8349.0
33	8615.0
34	8881.0
35	8881.0
36	9272.5
37	9664.0
38	10728.5
39	11793.0
40	11793.0
41	12742.0
42	13691.0
43	15044.0
44	16397.0
45	16397.0
46	16752.5
47	17108.0
48	17108.0
49	17130.5
50	17153.0
51	17070.0
52	16987.0
53	16987.0
54	16753.0
55	16519.0
56	16519.0
57	16661.5
58	16804.0
59	15721.5
60	14639.0
61	14639.0
62	13815.5
63	12992.0
64	11672.5
65	10353.0
66	10353.0
67	9467.5
68	8582.0
69	8582.0
70	7382.0
71	6182.0
72	5221.0
73	4260.0
74	4260.0
75	3227.0
76	2194.0
77	2194.0
78	1696.5
79	1199.0
80	920.0
81	641.0
82	641.0
83	448.0
84	255.0
85	255.0
86	159.5
87	64.0
88	45.0
89	26.0
90	26.0
91	19.5
92	13.0
93	7.5
94	2.0
95	2.0
96	5.0
97	8.0
98	8.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01188043529914936
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.9601450997164535E-4
8	0.0027721015698015175
9	0.0027721015698015175
10	7.920290199432907E-4
11	0.006732246669517971
12	0.0
13	3.9601450997164535E-4
14	0.0
15	0.0
16	3.9601450997164535E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	252516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.33841815964137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.208972436858495	19.988040361798856
2	16.340675815938184	11.222259183576485
3	8.143236074270558	8.388775364729364
4	4.844885249682851	6.654627825563529
5	3.1726444470072654	5.447179584659982
6	2.138161688386576	4.405265408924583
7	1.5165494175988927	3.6453135642889953
8	1.0909929650559336	2.9970378114654124
9	0.8119017414369737	2.509147935180345
>10	3.5682158920539733	19.828446514280284
>50	0.10725406527505477	2.5832026485450426
>100	0.04267097220620459	2.653297216810024
>500	0.004613078076346442	1.0035007682681494
>1k	0.006919617114519662	4.1854773558903196
>5k	0.002306539038173221	4.488428456018628
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6237	2.469942498693152	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5097	2.0184859573254763	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2968	1.1753710655958434	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2157	0.854203298008839	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1796	0.7112420599090751	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1296	0.5132348049232524	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1281	0.5072945872736777	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1071	0.42413154017963217	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	721	0.2855264616895563	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	662	0.26216160560122925	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.25146921383199483	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	516	0.204343487145369	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	381	0.15088152829919688	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	367	0.14533732515959386	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	332	0.13147681731058625	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	292	0.11563623691172044	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.11365616436186222	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	277	0.10969601926214577	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	274	0.10850797573223082	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	272	0.10771594671228754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	3.9601450997164535E-4	0.0	0.0
10	0.0	0.0	3.9601450997164535E-4	0.0	0.0
11	0.0	0.0	3.9601450997164535E-4	0.0	0.0
12	0.0	0.0	3.9601450997164535E-4	0.0	0.0
13	0.0	0.0	3.9601450997164535E-4	0.0	0.0
14	0.0	0.0	3.9601450997164535E-4	0.0	0.0
15	0.0	0.0	3.9601450997164535E-4	0.0	0.0
16	0.0	0.0	3.9601450997164535E-4	0.0	0.0
17	0.0	0.0	3.9601450997164535E-4	0.0	0.0
18	0.0	0.0	3.9601450997164535E-4	0.0	0.0
19	0.0	0.0	3.9601450997164535E-4	0.0	0.0
20	0.0	0.0	3.9601450997164535E-4	0.0	0.0
21	0.0	0.0	3.9601450997164535E-4	7.920290199432907E-4	0.0
22	0.0	0.0	3.9601450997164535E-4	0.001188043529914936	0.0
23	0.0	0.0	3.9601450997164535E-4	0.003168116079773163	0.0
24	0.0	0.0	3.9601450997164535E-4	0.005940217649574681	0.0
25	0.0	0.0	3.9601450997164535E-4	0.006732246669517971	0.0
26	0.0	0.0	3.9601450997164535E-4	0.006732246669517971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCC	20	0.0037454036	32.004555	4
TACTGGT	30	2.2372165E-5	32.004555	7
CATAGTA	30	8.391614E-4	26.670464	2
TTTCGTC	30	8.4013294E-4	26.665184	17
TATGGGT	35	0.0020603985	22.860397	4
GTACTGG	35	0.0020603985	22.860397	6
TAGGACT	35	0.0020603985	22.860397	4
TCCCAAG	35	0.0020603985	22.860397	2
ACCTAGG	40	0.0044651544	20.00681	1
CCCTTAC	40	0.0044651544	20.00681	1
ATACTGC	40	0.004470278	20.002848	6
GGACCGT	40	0.004470278	20.002848	6
TAGGACC	40	0.004470278	20.002848	4
ATTGAGC	40	0.004470278	20.002848	8
CTTAGGG	40	0.004470278	20.002848	3
GCTCCGG	40	0.0044754064	19.998886	21
GGTACTG	50	7.167809E-4	19.202734	5
AGATCTT	45	0.0088244565	17.780308	5
GTAGAAC	45	0.0088244565	17.780308	3
AGTAGCT	45	0.0088244565	17.780308	7
>>END_MODULE