FastQCFastQC Report
Thu 2 Feb 2017
SRR4062903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378172
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25630.1859709818513219No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20000.145119767343989No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19730.14316065048484514No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17610.12777795514638232No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17580.12756027549536633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17480.1268346766586464No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17390.12618163770559843No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17370.12603651793825443No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17140.12436764061379857No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16870.1224085237546547No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16590.12037684701183887No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16420.11914332898941497No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15660.11362877783034338No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14800.10738862783455186No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14580.10579231039376798No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14490.10513927144072002No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA13930.10107591795508833No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13860.10056799876938438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG507.2086905E-419.1953435
ACCGTCG3300.018.4224458
TACCGTC3300.017.9376437
ATACCGT3500.017.8242456
TAGCGCT450.00885611817.7760437
CTAGCGG4100.017.559929
TCTAGCG4150.017.34833528
CCGTCGT3700.016.8650579
CGTCGTA3800.016.4212410
CGCCGGT4350.016.182337
TAGCGGC4350.015.81512330
GTATTAC951.4179557E-515.1745861
TAGGACG951.4360981E-515.1542164
CGGTCCA4800.015.00017110
ATCGTTT3550.014.87232929
ACGCGTA650.004168857414.7656466
GCCGGTC4900.014.6924458
TCGTTTA3650.014.46486830
CCGGTCC5450.014.3854859
CATCGTT3800.014.31491328
Produced by FastQC (version 0.11.5)