FastQCFastQC Report
Thu 2 Feb 2017
SRR4062903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378172
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26980.19576656614704113No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20360.14773192315618078No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19540.14178201269507726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19180.13916985688288544No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18790.13634002141967766No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18700.13568698246662972No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18070.13111570979529405No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17650.12806819468107028No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17460.1266895568913024No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17430.1264718772402864No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16910.1226987632893427No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16860.12233596387098272No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16460.11943356852410295No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15720.11406413713237534No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15450.1121050202732315No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15150.10992822376307165No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15140.10985566387939967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC3450.019.9442447
ACCGTCG3700.018.596668
TCGTTTA3800.018.10464730
ATCGTTT4050.017.77717429
TACGATT450.00885287517.77717424
TAGCGTA3700.017.73177
CCGTCGT3850.017.455859
CGTCGTA3850.017.45521710
ATACCGT3950.017.4190256
AGCGTAT3850.017.0408558
CATCGTT4150.016.96327828
CGTTTAT4100.016.77991731
GTATAAT1151.8408173E-816.6962951
AATAGCG4050.016.5938365
ATAGCGT4000.016.4012286
CGGCATC4500.016.35499825
CGCGTAA1501.1459633E-1016.00061610
GTCGTAG4300.015.62850911
TCGCGTA1551.891749E-1015.4850299
CCAATAG4550.015.4731233
Produced by FastQC (version 0.11.5)