##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062903_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1378172
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226591455928578	32.0	32.0	32.0	32.0	32.0
2	31.26885178337682	32.0	32.0	32.0	32.0	32.0
3	31.36422086648111	32.0	32.0	32.0	32.0	32.0
4	31.466930107417653	32.0	32.0	32.0	32.0	32.0
5	31.38898700597603	32.0	32.0	32.0	32.0	32.0
6	34.91650824425398	36.0	36.0	36.0	36.0	36.0
7	34.940716398243474	36.0	36.0	36.0	36.0	36.0
8	34.876857170222586	36.0	36.0	36.0	32.0	36.0
9	34.996673854932475	36.0	36.0	36.0	36.0	36.0
10	34.83577448968634	36.0	36.0	36.0	32.0	36.0
11	35.01402872790914	36.0	36.0	36.0	36.0	36.0
12	34.90468025761661	36.0	36.0	36.0	32.0	36.0
13	34.95780715324357	36.0	36.0	36.0	36.0	36.0
14	34.9102564846768	36.0	36.0	36.0	32.0	36.0
15	34.88087190858616	36.0	36.0	36.0	32.0	36.0
16	34.89346902999045	36.0	36.0	36.0	32.0	36.0
17	34.85622259050394	36.0	36.0	36.0	32.0	36.0
18	34.85664343782924	36.0	36.0	36.0	32.0	36.0
19	34.85245673254137	36.0	36.0	36.0	32.0	36.0
20	34.841889111083375	36.0	36.0	36.0	32.0	36.0
21	34.825194533048126	36.0	36.0	36.0	32.0	36.0
22	34.81313290358533	36.0	36.0	36.0	32.0	36.0
23	34.76028463791167	36.0	36.0	36.0	32.0	36.0
24	34.72844173296222	36.0	36.0	36.0	32.0	36.0
25	34.711053482439056	36.0	36.0	36.0	32.0	36.0
26	34.64492313005924	36.0	36.0	36.0	32.0	36.0
27	34.63382364465393	36.0	36.0	36.0	32.0	36.0
28	34.601948087756824	36.0	36.0	36.0	32.0	36.0
29	34.57000069657488	36.0	36.0	36.0	32.0	36.0
30	34.54829368177557	36.0	36.0	36.0	32.0	36.0
31	34.54798747906647	36.0	36.0	36.0	32.0	36.0
32	34.51792809605767	36.0	36.0	36.0	32.0	36.0
33	34.483299617174055	36.0	36.0	36.0	32.0	36.0
34	34.471597159135435	36.0	36.0	36.0	32.0	36.0
35	34.44115103194667	36.0	36.0	36.0	32.0	36.0
36	34.415185477574646	36.0	36.0	36.0	32.0	36.0
37	34.41539227324311	36.0	36.0	36.0	32.0	36.0
38	33.96943850259619	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	21.0
22	94.0
23	285.0
24	865.0
25	2055.0
26	4655.0
27	8857.0
28	15831.0
29	24673.0
30	38124.0
31	55595.0
32	80751.0
33	129681.0
34	318800.0
35	697881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.559682332810375	18.680726187659918	12.1033695537014	26.656221925828312
2	15.80419570271345	20.967049105626874	36.66879025259547	26.559964939064212
3	17.445427711490293	25.100713118536728	29.13090673733032	28.322952432642662
4	12.048757516324219	16.36507041599458	36.54179790983241	35.04437415784879
5	14.190754129382979	37.03035615293302	33.34591037983648	15.432979337847524
6	33.83099774991638	36.29701974573547	16.65083650722588	13.221145997122271
7	29.495226250614948	30.915523719272482	21.444140819439227	18.145109210673347
8	27.855176447197906	33.02356934606348	19.619837085475705	19.501417121262914
9	27.636369969073943	13.810130568612738	18.492721275122882	40.06077818719043
10	15.792628152568422	26.671900690920225	31.512333092916506	26.023138063594846
11	37.689764043500695	20.994156417600262	22.226656691529527	19.089422847369516
12	24.48747103034032	23.96111934266264	28.690230349609553	22.861179277387482
13	29.43330648613741	19.219689878607138	25.288897596087565	26.058106039167882
14	23.6573690782929	19.80479925930817	25.002485177819008	31.535346484579925
15	25.185390502781946	27.120635160197708	22.18707098968779	25.506903347332553
16	25.94770464063992	25.625901556554627	23.527905080062574	24.89848872274288
17	24.223464124942314	25.828706431417846	24.933535146556455	25.014294297083385
18	25.304098472469327	24.521104767764836	25.913601495314083	24.26119526445175
19	25.741924810546145	24.93382538609114	25.250984637621425	24.073265165741287
20	25.95771790458666	24.060276946564	24.751845197841778	25.23015995100757
21	27.179705231873037	24.259814478350968	24.122966140557608	24.437514149218384
22	25.892631688932877	24.306545191746746	24.75960910539468	25.041214013925696
23	24.258998714961663	23.761236829445444	25.491068221676734	26.48869623391616
24	24.831878749531988	25.05681438891517	24.992526331981786	25.118780529571055
25	25.015763646165823	24.299234274992003	25.118363396124284	25.56663868271789
26	24.86721541288025	25.203167674281584	25.425346038085234	24.504270874752933
27	25.694978565810363	24.533802747407435	24.464580618384353	25.306638068397845
28	24.557747509019194	24.49070217650627	25.631416107713694	25.32013420676084
29	24.73007723274018	24.74175937401137	25.42229852297101	25.10586487027744
30	24.630452512458533	24.802637116412175	25.7200117256772	24.846898645452093
31	25.324705479432176	24.716145735075155	24.379612994604447	25.57953579088822
32	24.982658187802393	24.710340944381397	24.367495494031225	25.939505373784982
33	24.532061310199307	24.183193389504357	25.25867598529066	26.026069315005675
34	25.363234777662	24.319170611505676	25.43731841889111	24.88027619194121
35	26.22843883056687	24.17165636800051	25.246558484717436	24.353346316715186
36	24.908501986689615	24.98302098722075	24.74974096121529	25.358736064874343
37	25.840606252340052	24.83594210301762	24.538446579962443	24.78500506467988
38	24.752153397510178	24.490357147262568	25.338582802859733	25.418906652367525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	159.0
1	129.0
2	99.0
3	99.0
4	341.0
5	583.0
6	583.0
7	711.5
8	840.0
9	839.0
10	838.0
11	838.0
12	1174.5
13	1511.0
14	1976.0
15	2441.0
16	2441.0
17	3790.0
18	5139.0
19	5139.0
20	6346.0
21	7553.0
22	8692.5
23	9832.0
24	9832.0
25	11897.0
26	13962.0
27	13962.0
28	17726.0
29	21490.0
30	26136.5
31	30783.0
32	30783.0
33	40305.0
34	49827.0
35	49827.0
36	54666.5
37	59506.0
38	67964.5
39	76423.0
40	76423.0
41	81513.0
42	86603.0
43	97973.5
44	109344.0
45	109344.0
46	111330.5
47	113317.0
48	113317.0
49	120861.0
50	128405.0
51	129260.5
52	130116.0
53	130116.0
54	123470.5
55	116825.0
56	116825.0
57	113942.5
58	111060.0
59	98662.5
60	86265.0
61	86265.0
62	82046.0
63	77827.0
64	64120.0
65	50413.0
66	50413.0
67	42512.5
68	34612.0
69	34612.0
70	27869.5
71	21127.0
72	16468.5
73	11810.0
74	11810.0
75	8930.0
76	6050.0
77	6050.0
78	5894.5
79	5739.0
80	4471.0
81	3203.0
82	3203.0
83	3105.5
84	3008.0
85	3008.0
86	1898.0
87	788.0
88	642.0
89	496.0
90	496.0
91	301.0
92	106.0
93	68.0
94	30.0
95	30.0
96	26.5
97	23.0
98	23.0
99	21.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009142545342671306
2	0.0
3	0.0
4	3.627994183599725E-4
5	0.0
6	7.25598836719945E-5
7	7.25598836719945E-4
8	0.0018865569754718569
9	0.003337754648911747
10	7.25598836719945E-4
11	0.005732230810087565
12	4.35359302031967E-4
13	1.45119767343989E-4
14	7.25598836719945E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.90239534687978E-4
22	0.0
23	2.176796510159835E-4
24	0.0
25	7.25598836719945E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.25598836719945E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1378172.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.98943608405644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.27653402008983	46.066167427355154
2	11.221495444280642	12.565704038901632
3	2.942363100850114	4.942237523134008
4	1.1826549328684188	2.648647310933216
5	0.6023597995758446	1.6862892748978404
6	0.3818878117295573	1.282900993566734
7	0.24653684213471355	0.9662421135546662
8	0.18428496240596007	0.8254408899104997
9	0.1335513465209092	0.6729718121974908
>10	0.6600435724101372	6.719126323864353
>50	0.06934703322370375	2.7702107418493944
>100	0.08149627659895829	9.965474816007806
>500	0.012888364729994157	5.066228389311965
>1k	0.004556492581311065	3.822358344515062
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2698	0.19576656614704113	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2036	0.14773192315618078	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1954	0.14178201269507726	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1918	0.13916985688288544	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1879	0.13634002141967766	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1870	0.13568698246662972	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1807	0.13111570979529405	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1765	0.12806819468107028	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1746	0.1266895568913024	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1743	0.1264718772402864	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1691	0.1226987632893427	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1686	0.12233596387098272	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1646	0.11943356852410295	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1572	0.11406413713237534	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1545	0.1121050202732315	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1515	0.10992822376307165	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1514	0.10985566387939967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.25598836719945E-5
2	0.0	0.0	0.0	0.0	7.25598836719945E-5
3	0.0	0.0	0.0	0.0	7.25598836719945E-5
4	0.0	0.0	0.0	0.0	7.25598836719945E-5
5	0.0	0.0	0.0	0.0	7.25598836719945E-5
6	0.0	0.0	0.0	0.0	2.176796510159835E-4
7	0.0	0.0	0.0	0.0	2.176796510159835E-4
8	0.0	0.0	0.0	0.0	2.176796510159835E-4
9	0.0	0.0	0.0	0.0	2.176796510159835E-4
10	0.0	0.0	0.0	0.0	2.176796510159835E-4
11	0.0	0.0	0.0	0.0	2.176796510159835E-4
12	0.0	0.0	0.0	0.0	5.80479069375956E-4
13	0.0	0.0	0.0	0.0	6.530389530479504E-4
14	0.0	0.0	0.0	0.0	6.530389530479504E-4
15	0.0	0.0	0.0	0.0	9.432784877359284E-4
16	0.0	0.0	0.0	1.45119767343989E-4	0.0010158383714079229
17	0.0	0.0	0.0	1.45119767343989E-4	0.0010158383714079229
18	0.0	0.0	0.0	2.90239534687978E-4	0.001160958138751912
19	0.0	0.0	0.0	2.90239534687978E-4	0.0012335180224239066
20	0.0	0.0	0.0	2.90239534687978E-4	0.0013060779060959009
21	0.0	0.0	0.0	3.627994183599725E-4	0.0013060779060959009
22	0.0	0.0	0.0	5.079191857039614E-4	0.0013786377897678954
23	0.0	0.0	0.0	7.981587203919394E-4	0.0013786377897678954
24	0.0	0.0	0.0	0.0013060779060959009	0.0013786377897678954
25	0.0	0.0	0.0	0.0018139970917998623	0.0013786377897678954
26	0.0	0.0	0.0	0.002176796510159835	0.0013786377897678954
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	345	0.0	19.944244	7
ACCGTCG	370	0.0	18.59666	8
TCGTTTA	380	0.0	18.104647	30
ATCGTTT	405	0.0	17.777174	29
TACGATT	45	0.008852875	17.777174	24
TAGCGTA	370	0.0	17.7317	7
CCGTCGT	385	0.0	17.45585	9
CGTCGTA	385	0.0	17.455217	10
ATACCGT	395	0.0	17.419025	6
AGCGTAT	385	0.0	17.040855	8
CATCGTT	415	0.0	16.963278	28
CGTTTAT	410	0.0	16.779917	31
GTATAAT	115	1.8408173E-8	16.696295	1
AATAGCG	405	0.0	16.593836	5
ATAGCGT	400	0.0	16.401228	6
CGGCATC	450	0.0	16.354998	25
CGCGTAA	150	1.1459633E-10	16.000616	10
GTCGTAG	430	0.0	15.628509	11
TCGCGTA	155	1.891749E-10	15.485029	9
CCAATAG	455	0.0	15.473123	3
>>END_MODULE