FastQCFastQC Report
Thu 2 Feb 2017
SRR4062902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683397
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT18540.2712917967155255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12870.18832391713747645No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10830.15847303982897204No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10190.14910805871257848No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9750.14266963419505793No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9530.13945042193629764No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9420.13784081580691748No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9220.13491425920804453No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9110.13330465307866438No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8930.1306707521396787No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8600.1258419337515383No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8430.12335436064249622No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG7660.11208711773683525No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7510.10989220028768051No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7370.10784361066846941No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA7320.10711197151875118No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT7310.10696564368880754No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT7300.10681931585886388No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7210.10550236538937105No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT6920.10125885832100522No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA6840.10008823568145601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC350.002066210122.8561518
CGTCGTA1750.019.20197510
TATACTG953.7876816E-818.5241555
ATACCGT2100.018.2835836
CCGTCGT1850.018.1640329
TACCGTC2050.017.950447
ACGTGCC450.00884146117.7796089
GAGTGCG450.008848919517.7770067
TCGTACT450.0088526517.7757034
TCGACGC550.001362937717.45634314
TGCGTAG550.001362937717.45634313
TAGTACG550.001363606417.4550652
GTATAGG652.0948377E-417.2350651
TGTTAAG652.0983562E-417.2312812
ACCGTCG2050.017.1699858
GTAATAC753.2338277E-517.0646763
TCTAGCG1900.016.84137328
CTAGCGG2000.016.7992729
GTATATG1051.1836164E-716.7660851
CTATAGA805.603543E-516.003991
Produced by FastQC (version 0.11.5)