##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062902_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683397
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191671897886586	32.0	32.0	32.0	32.0	32.0
2	30.775398487262894	32.0	32.0	32.0	32.0	32.0
3	30.833667692424754	32.0	32.0	32.0	32.0	32.0
4	30.898907955405132	32.0	32.0	32.0	32.0	32.0
5	30.81924562150551	32.0	32.0	32.0	32.0	32.0
6	34.463582661322775	36.0	36.0	36.0	32.0	36.0
7	34.371780970651024	36.0	36.0	36.0	32.0	36.0
8	34.34723886701288	36.0	36.0	36.0	32.0	36.0
9	34.49608499890986	36.0	36.0	36.0	32.0	36.0
10	34.18945503126294	36.0	36.0	36.0	32.0	36.0
11	34.494500268511565	36.0	36.0	36.0	32.0	36.0
12	34.27927544311725	36.0	36.0	36.0	32.0	36.0
13	34.38940615776774	36.0	36.0	36.0	32.0	36.0
14	34.26558354806942	36.0	36.0	36.0	32.0	36.0
15	34.226714486601495	36.0	36.0	36.0	32.0	36.0
16	34.23949914910367	36.0	36.0	36.0	32.0	36.0
17	34.15224240082997	36.0	36.0	36.0	32.0	36.0
18	34.173136551667625	36.0	36.0	36.0	32.0	36.0
19	34.1667127599331	36.0	36.0	36.0	32.0	36.0
20	34.15205802776424	36.0	36.0	36.0	32.0	36.0
21	34.13284957352754	36.0	36.0	36.0	32.0	36.0
22	34.106528123477275	36.0	36.0	36.0	32.0	36.0
23	34.046081560205856	36.0	36.0	36.0	32.0	36.0
24	34.03508941362049	36.0	36.0	36.0	32.0	36.0
25	34.000640915895154	36.0	36.0	36.0	32.0	36.0
26	33.9613109217629	36.0	36.0	36.0	32.0	36.0
27	33.98213044540728	36.0	36.0	36.0	32.0	36.0
28	33.94945983081576	36.0	36.0	36.0	32.0	36.0
29	33.921222949471534	36.0	36.0	36.0	32.0	36.0
30	33.8872983053774	36.0	36.0	36.0	32.0	36.0
31	33.90796711135694	36.0	36.0	36.0	32.0	36.0
32	33.86902342269574	36.0	36.0	36.0	32.0	36.0
33	33.81184435986696	36.0	36.0	36.0	32.0	36.0
34	33.81010013213403	36.0	36.0	36.0	32.0	36.0
35	33.769350758051324	36.0	36.0	36.0	32.0	36.0
36	33.7263201330998	36.0	36.0	36.0	27.0	36.0
37	33.734387186364586	36.0	36.0	36.0	27.0	36.0
38	33.13921629740839	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	0.0
9	2.0
10	7.0
11	3.0
12	2.0
13	5.0
14	70.0
15	174.0
16	261.0
17	276.0
18	396.0
19	440.0
20	626.0
21	946.0
22	1387.0
23	2117.0
24	3396.0
25	5029.0
26	7278.0
27	10427.0
28	14516.0
29	19559.0
30	26061.0
31	34361.0
32	46318.0
33	68606.0
34	147418.0
35	293713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4712735285155	17.234124844705935	11.308777337615151	25.985824289163407
2	17.03483979783664	20.012239902080783	36.7240985517092	26.22882174837338
3	18.87960732547555	23.981215078280265	28.56344228275727	28.575735313486916
4	12.63852285215498	15.172724625423086	36.010015057706575	36.17873746471536
5	14.907901129067188	36.26462548800403	33.232854743077226	15.594618639851564
6	34.024589036485544	35.68805110960881	16.82484774522457	13.462512108681082
7	30.201771444709298	30.509352543250852	21.012969035567906	18.275906976471948
8	28.438729162764563	32.07397803895861	19.831809561715676	19.655483236561153
9	27.930154493601055	13.870768546402221	18.717899191388863	39.481177768607864
10	16.21012183308604	26.02320184256812	31.062900757103517	26.70377556724232
11	37.93471294239532	20.965141019224973	21.923609342944356	19.17653669543535
12	24.894019587901916	23.906137279204426	28.27953952480388	22.920303608089775
13	29.766617542247243	18.810067042392863	25.18282625907546	26.240489156284436
14	23.97000566345055	19.647783132283276	24.804010952264573	31.578200252001597
15	25.623503445368804	26.804208209169307	22.224677856553228	25.34761048890866
16	26.315458271929426	25.362313538226033	23.445779923520124	24.876448266324413
17	24.389023051591657	25.436955726308085	25.091255031101355	25.0827661909989
18	25.609672222067758	24.007293384589946	26.156968738247393	24.226065655094907
19	25.801754604189686	24.753967615187058	25.400785840241753	24.043491940381507
20	26.075351426338756	23.50027364865854	24.93934365296082	25.485031272041887
21	27.629148996572805	23.780946443884467	24.076838201938074	24.51306635760465
22	26.214799888196705	23.92297865054592	24.773356864707768	25.08886459654961
23	24.349621633961775	23.565084665894958	25.495036826847233	26.590256873296035
24	24.77282938458679	24.820246539937976	25.141336783272866	25.26558729220237
25	25.0390393466855	24.073702774098656	25.171048683931303	25.716209195284545
26	24.80172465522263	25.04643133650535	25.604046441581403	24.547797566690623
27	25.89593683917844	24.327306518764587	24.51301338289419	25.263743259162787
28	24.59494263638492	24.3157340201358	25.705923671271364	25.38339967220791
29	24.48304323265908	24.746899722826292	25.727398050125416	25.04265899438921
30	24.650778924694677	24.816426633576146	25.857724421915833	24.67507001981334
31	25.36038784771153	24.783825825588526	24.343355854372724	25.512430472327218
32	24.832188247689707	24.631121452246635	24.353520498130546	26.183169801933108
33	24.54378093289665	23.991342447470785	25.27592656007165	26.188950059560916
34	25.31892505755066	24.48416606786661	25.242093278170557	24.954815596412175
35	26.381994122536913	24.198141925513106	25.047417779911253	24.37244617203873
36	24.80839112942764	25.092895727016085	24.803854276276795	25.294858867279483
37	26.001416588129928	24.92580473327753	24.565072322092334	24.507706356500208
38	24.77969087945118	24.47414093344923	25.349084959859987	25.3970832272396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	267.0
1	208.0
2	149.0
3	149.0
4	339.0
5	529.0
6	529.0
7	652.0
8	775.0
9	704.5
10	634.0
11	634.0
12	854.0
13	1074.0
14	1361.5
15	1649.0
16	1649.0
17	2383.5
18	3118.0
19	3118.0
20	3708.5
21	4299.0
22	4477.5
23	4656.0
24	4656.0
25	5373.5
26	6091.0
27	6091.0
28	7967.5
29	9844.0
30	12044.5
31	14245.0
32	14245.0
33	18300.0
34	22355.0
35	22355.0
36	24982.5
37	27610.0
38	31944.0
39	36278.0
40	36278.0
41	38824.5
42	41371.0
43	47309.0
44	53247.0
45	53247.0
46	54890.5
47	56534.0
48	56534.0
49	59924.0
50	63314.0
51	64022.0
52	64730.0
53	64730.0
54	61076.5
55	57423.0
56	57423.0
57	56136.0
58	54849.0
59	48533.0
60	42217.0
61	42217.0
62	41143.5
63	40070.0
64	33303.0
65	26536.0
66	26536.0
67	22409.0
68	18282.0
69	18282.0
70	14902.5
71	11523.0
72	9178.5
73	6834.0
74	6834.0
75	5267.5
76	3701.0
77	3701.0
78	3520.0
79	3339.0
80	2610.0
81	1881.0
82	1881.0
83	1924.0
84	1967.0
85	1967.0
86	1334.0
87	701.0
88	592.0
89	483.0
90	483.0
91	358.5
92	234.0
93	194.5
94	155.0
95	155.0
96	135.5
97	116.0
98	116.0
99	216.5
100	317.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12013514838373596
2	0.05633621452830492
3	0.01214520988532288
4	0.0038045235785348777
5	1.4632782994364915E-4
6	4.3898348983094745E-4
7	0.0
8	7.316391497182458E-4
9	4.3898348983094745E-4
10	4.3898348983094745E-4
11	0.0021949174491547375
12	0.0023412452790983864
13	0.014340127334477619
14	0.009950292436168143
15	0.023558780620927516
16	0.011706226395491933
17	0.021510191001716427
18	0.005560457537858668
19	0.0076090471570697555
20	0.005706785367802317
21	0.005706785367802317
22	0.007901702816957054
23	0.009364981116393545
24	0.014632782994364915
25	0.016388716953688705
26	0.01902261789267439
27	0.009072325456506247
28	0.0054141297079150184
29	0.01038927592599909
30	0.002780228768929334
31	0.00526780187797137
32	0.006145768857633265
33	0.009218653286449895
34	0.012584193375153827
35	0.01594973346385776
36	0.015218094314139511
37	0.009511308946337196
38	0.005706785367802317
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	683397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.91070239739974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.9008326453975	50.01567685264453
2	9.81476199676024	11.563890461849025
3	2.3473849071790975	4.148582810369269
4	0.8844337456325908	2.084104527167164
5	0.47396535749307567	1.396081606097588
6	0.2857075329479917	1.0098738867716652
7	0.19596844059935598	0.8081246938401817
8	0.14369405837796165	0.6772094327502947
9	0.10646141697801864	0.5644545167157783
>10	0.6477360158599247	7.45441585584158
>50	0.08833020572989646	3.716684845296526
>100	0.10077098697299619	12.082148894447032
>500	0.008957421064241121	3.710232724905718
>1k	9.952690071379023E-4	0.7685188913037564
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1854	0.2712917967155255	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1287	0.18832391713747645	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1083	0.15847303982897204	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1019	0.14910805871257848	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	975	0.14266963419505793	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	953	0.13945042193629764	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	942	0.13784081580691748	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	922	0.13491425920804453	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	911	0.13330465307866438	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	893	0.1306707521396787	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	860	0.1258419337515383	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	843	0.12335436064249622	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	766	0.11208711773683525	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	751	0.10989220028768051	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	737	0.10784361066846941	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	732	0.10711197151875118	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	731	0.10696564368880754	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	730	0.10681931585886388	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	721	0.10550236538937105	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	692	0.10125885832100522	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	684	0.10008823568145601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.926556598872983E-4	0.0
8	0.0	0.0	0.0	2.926556598872983E-4	0.0
9	0.0	0.0	0.0	2.926556598872983E-4	0.0
10	0.0	0.0	0.0	4.3898348983094745E-4	0.0
11	0.0	0.0	0.0	4.3898348983094745E-4	0.0
12	0.0	0.0	0.0	4.3898348983094745E-4	2.926556598872983E-4
13	0.0	0.0	0.0	4.3898348983094745E-4	2.926556598872983E-4
14	0.0	0.0	0.0	4.3898348983094745E-4	2.926556598872983E-4
15	0.0	0.0	0.0	4.3898348983094745E-4	4.3898348983094745E-4
16	0.0	0.0	0.0	5.853113197745966E-4	4.3898348983094745E-4
17	0.0	0.0	0.0	7.316391497182458E-4	4.3898348983094745E-4
18	0.0	0.0	0.0	7.316391497182458E-4	5.853113197745966E-4
19	0.0	0.0	0.0	7.316391497182458E-4	5.853113197745966E-4
20	0.0	0.0	0.0	7.316391497182458E-4	5.853113197745966E-4
21	0.0	0.0	0.0	0.001024294809605544	5.853113197745966E-4
22	0.0	0.0	0.0	0.0017559339593237898	5.853113197745966E-4
23	0.0	0.0	0.0	0.0024875731090420357	5.853113197745966E-4
24	0.0	0.0	0.0	0.003658195748591229	5.853113197745966E-4
25	0.0	0.0	0.0	0.004243507068365826	5.853113197745966E-4
26	0.0	0.0	0.0	0.005121474048027721	5.853113197745966E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAC	35	0.0020662101	22.85615	18
CGTCGTA	175	0.0	19.201975	10
TATACTG	95	3.7876816E-8	18.524155	5
ATACCGT	210	0.0	18.283583	6
CCGTCGT	185	0.0	18.164032	9
TACCGTC	205	0.0	17.95044	7
ACGTGCC	45	0.008841461	17.779608	9
GAGTGCG	45	0.0088489195	17.777006	7
TCGTACT	45	0.00885265	17.775703	4
TCGACGC	55	0.0013629377	17.456343	14
TGCGTAG	55	0.0013629377	17.456343	13
TAGTACG	55	0.0013636064	17.455065	2
GTATAGG	65	2.0948377E-4	17.235065	1
TGTTAAG	65	2.0983562E-4	17.231281	2
ACCGTCG	205	0.0	17.169985	8
GTAATAC	75	3.2338277E-5	17.064676	3
TCTAGCG	190	0.0	16.841373	28
CTAGCGG	200	0.0	16.79927	29
GTATATG	105	1.1836164E-7	16.766085	1
CTATAGA	80	5.603543E-5	16.00399	1
>>END_MODULE