FastQCFastQC Report
Thu 2 Feb 2017
SRR4062902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683397
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20690.3027522801534101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15800.23119797131096564No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11440.16739903745553464No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10880.15920467897869028No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10650.15583913888998635No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9880.14457189598432535No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9780.14310861768488886No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9470.13857245495663575No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9150.13388996439843898No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9090.1330119974187771No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9030.1321340304391152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8930.1306707521396787No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8500.12437865545210178No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8490.12423232762215812No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8340.12203741017300339No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8170.11954983706396136No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG7980.11676960829503202No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG7620.11150180641706064No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7530.11018485594756781No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7330.10725829934869482No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7130.10433174274982185No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA6840.10008823568145601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGT350.002063300522.861696
GCTATAC453.5317393E-421.3360181
ATCGTTT1700.020.70394129
CGGCATC1750.019.198225
GGCATCG1750.019.198226
CTAGCGG2450.018.93729
TCTAGCG2500.018.5582628
AGCGGCG2500.018.5582631
GAGCGTT701.7813554E-518.2906917
CATCGTT1850.018.16045828
TAGCGGC2600.017.8444830
TATAAGG450.008840295517.7800142
TCGCCAA450.00885148317.77611222
TCGTTTA1900.017.68255230
TCAATAC550.001362060917.458023
CCCATAT550.001362729217.456741
TAAGAGG3500.017.3748864
GATATAT753.2271062E-517.0688131
GCATTCG3500.016.455622
CGTTTAT2050.016.38870631
Produced by FastQC (version 0.11.5)