##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062902_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683397
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231490626970853	32.0	32.0	32.0	32.0	32.0
2	31.326107665090717	32.0	32.0	32.0	32.0	32.0
3	31.38928031583399	32.0	32.0	32.0	32.0	32.0
4	31.48618738449247	32.0	32.0	32.0	32.0	32.0
5	31.415104251262445	32.0	32.0	32.0	32.0	32.0
6	34.93031868738083	36.0	36.0	36.0	36.0	36.0
7	34.95624505229025	36.0	36.0	36.0	36.0	36.0
8	34.90046488351573	36.0	36.0	36.0	36.0	36.0
9	35.002530008179725	36.0	36.0	36.0	36.0	36.0
10	34.84928818827124	36.0	36.0	36.0	32.0	36.0
11	35.03450117574411	36.0	36.0	36.0	36.0	36.0
12	34.919639682351544	36.0	36.0	36.0	32.0	36.0
13	34.97071248483678	36.0	36.0	36.0	36.0	36.0
14	34.91960017383746	36.0	36.0	36.0	32.0	36.0
15	34.891156970253014	36.0	36.0	36.0	32.0	36.0
16	34.89882454854206	36.0	36.0	36.0	32.0	36.0
17	34.8602891145264	36.0	36.0	36.0	32.0	36.0
18	34.85460427833309	36.0	36.0	36.0	32.0	36.0
19	34.84614652976235	36.0	36.0	36.0	32.0	36.0
20	34.83648303987287	36.0	36.0	36.0	32.0	36.0
21	34.82141712650187	36.0	36.0	36.0	32.0	36.0
22	34.8096538322527	36.0	36.0	36.0	32.0	36.0
23	34.735649995537	36.0	36.0	36.0	32.0	36.0
24	34.70581082445489	36.0	36.0	36.0	32.0	36.0
25	34.70220384344678	36.0	36.0	36.0	32.0	36.0
26	34.62987399710563	36.0	36.0	36.0	32.0	36.0
27	34.626801112676816	36.0	36.0	36.0	32.0	36.0
28	34.578668036295156	36.0	36.0	36.0	32.0	36.0
29	34.55428835654824	36.0	36.0	36.0	32.0	36.0
30	34.53148609080812	36.0	36.0	36.0	32.0	36.0
31	34.52293030259132	36.0	36.0	36.0	32.0	36.0
32	34.50070310522288	36.0	36.0	36.0	32.0	36.0
33	34.461286777670956	36.0	36.0	36.0	32.0	36.0
34	34.43943564282547	36.0	36.0	36.0	32.0	36.0
35	34.401538198148366	36.0	36.0	36.0	32.0	36.0
36	34.38461245805878	36.0	36.0	36.0	32.0	36.0
37	34.36006011147254	36.0	36.0	36.0	32.0	36.0
38	33.932162418038125	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	11.0
22	46.0
23	158.0
24	416.0
25	1071.0
26	2382.0
27	4429.0
28	7981.0
29	12707.0
30	19445.0
31	27660.0
32	39839.0
33	63737.0
34	153314.0
35	350198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83345626112204	18.45555867753114	11.936873653648028	26.774111407698793
2	16.04118835757254	20.905418080559322	36.12029318244008	26.933100379428065
3	17.748102493865208	24.61921840452914	29.093630788546044	28.539048313059613
4	12.0882883594748	16.09006185277372	35.983476661442765	35.83817312630872
5	14.260106878003384	36.85388852144291	33.0691136617715	15.8168909387822
6	33.95571801291497	36.00095406303547	16.602891747501072	13.440436176548488
7	29.989098544765476	30.741079463560606	21.004543492416538	18.265278499257384
8	28.287673437852106	32.34087324001369	19.685214505418607	19.68623881671559
9	27.752941675727172	13.820498149608776	18.58132282851578	39.845237346148274
10	16.0267957090576	26.25239064491442	31.144597618061642	26.57621602796634
11	37.968057644917145	20.97588958024807	21.8662015113645	19.189851263470285
12	24.724426501295742	24.02804828261337	28.27348246177529	22.974042754315597
13	29.594055552141224	18.84461964840195	25.298729576203478	26.26259522325335
14	23.934111504732975	19.60895350725859	24.850270048010163	31.606664939998275
15	25.479479716767848	26.857009907857364	22.14232722707299	25.52118314830179
16	26.119116880988475	25.366698078420125	23.438679769855252	25.075505270736148
17	24.38143568087071	25.687265235287832	24.975672998271868	24.955626085569588
18	25.680534155110422	24.078244417227467	26.04445146818028	24.196769959481824
19	25.66048724240815	24.85465988290847	25.42797232062769	24.056880554055695
20	26.13107754350692	23.439377111693496	24.990744764756066	25.43880058004352
21	27.616027673681177	23.78982518111833	23.984149676686766	24.609997468513725
22	26.000883821386132	23.96648502478797	24.82118713015587	25.211444023670026
23	24.19929294289109	23.423022669140586	25.6346247270982	26.743059660870127
24	24.915971243654862	24.809444583455882	25.12522004047428	25.14936413241498
25	24.980538370139712	23.945706442531122	25.214370584551272	25.85938460277789
26	24.771692003330422	25.035374752888877	25.6097114854177	24.583221758363003
27	25.91773156744908	24.17452812933039	24.56990592583813	25.3378343773824
28	24.596391263057928	24.221206707082413	25.859346763301566	25.323055266558093
29	24.59800086918731	24.53215334571267	25.733358501720083	25.13648728337994
30	24.66092183606308	24.6408749233608	25.994700005999444	24.703503234576683
31	25.26920662513883	24.58234379138334	24.474207525054982	25.67424205842285
32	24.95811365867863	24.409823279879777	24.490888897668558	26.141174163773034
33	24.63326587620373	23.860801261931204	25.373245712228766	26.132687149636304
34	25.40002370510845	24.16896767179253	25.495575778061653	24.935432845037365
35	26.424757498203828	23.991472014070883	25.237453486041055	24.346317001684234
36	24.838124838124838	24.91670288280458	24.83461297020619	25.410559308864393
37	25.945972838628208	24.82773556219884	24.594635329098605	24.631656270074348
38	24.813285415776505	24.210267546195762	25.33640817329923	25.640038864728503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	142.0
1	126.0
2	110.0
3	110.0
4	327.0
5	544.0
6	544.0
7	602.0
8	660.0
9	650.0
10	640.0
11	640.0
12	876.0
13	1112.0
14	1361.0
15	1610.0
16	1610.0
17	2472.0
18	3334.0
19	3334.0
20	3865.5
21	4397.0
22	4624.0
23	4851.0
24	4851.0
25	5527.0
26	6203.0
27	6203.0
28	7934.5
29	9666.0
30	11829.5
31	13993.0
32	13993.0
33	18444.5
34	22896.0
35	22896.0
36	25345.5
37	27795.0
38	32219.5
39	36644.0
40	36644.0
41	39013.5
42	41383.0
43	47277.0
44	53171.0
45	53171.0
46	54973.5
47	56776.0
48	56776.0
49	60272.0
50	63768.0
51	64143.5
52	64519.0
53	64519.0
54	60734.5
55	56950.0
56	56950.0
57	55852.0
58	54754.0
59	48664.0
60	42574.0
61	42574.0
62	41395.0
63	40216.0
64	33473.5
65	26731.0
66	26731.0
67	22646.0
68	18561.0
69	18561.0
70	15163.5
71	11766.0
72	9223.0
73	6680.0
74	6680.0
75	5052.0
76	3424.0
77	3424.0
78	3367.0
79	3310.0
80	2523.0
81	1736.0
82	1736.0
83	1689.5
84	1643.0
85	1643.0
86	1054.5
87	466.0
88	383.0
89	300.0
90	300.0
91	168.5
92	37.0
93	29.5
94	22.0
95	22.0
96	14.5
97	7.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010096620266111791
2	0.0
3	0.0
4	0.0
5	1.4632782994364915E-4
6	5.853113197745966E-4
7	2.926556598872983E-4
8	0.0016096061293801407
9	0.004097179238422176
10	5.853113197745966E-4
11	0.005999441027689615
12	5.853113197745966E-4
13	4.3898348983094745E-4
14	0.0
15	0.0
16	1.4632782994364915E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	5.853113197745966E-4
22	1.4632782994364915E-4
23	1.4632782994364915E-4
24	0.0
25	1.4632782994364915E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.4632782994364915E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	683397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.86984617008136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.77996023676157	47.64553450800163
2	10.54470429641424	11.993514224921476
3	2.475245275813357	4.223004542061787
4	0.990149989814761	2.2523871042429233
5	0.5201925430512753	1.4791634951074726
6	0.32121542913740103	1.09604832255004
7	0.20366010663961567	0.8107483254904115
8	0.1396471153281819	0.6353367973447452
9	0.11117723397338732	0.5690368974313513
>10	0.698147503754675	7.690559097488822
>50	0.09724543642341679	3.9275626893299433
>100	0.10833702133272026	12.950359282494542
>500	0.009028085111049232	3.70781451738903
>1k	0.0012897264444356046	1.018930196145917
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2069	0.3027522801534101	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1580	0.23119797131096564	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1144	0.16739903745553464	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1088	0.15920467897869028	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1065	0.15583913888998635	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	988	0.14457189598432535	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	978	0.14310861768488886	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	947	0.13857245495663575	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	915	0.13388996439843898	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	909	0.1330119974187771	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	903	0.1321340304391152	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	893	0.1306707521396787	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	850	0.12437865545210178	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	849	0.12423232762215812	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	834	0.12203741017300339	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	817	0.11954983706396136	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	798	0.11676960829503202	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	762	0.11150180641706064	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	753	0.11018485594756781	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	733	0.10725829934869482	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	713	0.10433174274982185	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	684	0.10008823568145601	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	1.4632782994364915E-4
12	0.0	0.0	0.0	0.0	1.4632782994364915E-4
13	0.0	0.0	0.0	0.0	1.4632782994364915E-4
14	0.0	0.0	0.0	0.0	4.3898348983094745E-4
15	0.0	0.0	0.0	0.0	4.3898348983094745E-4
16	0.0	0.0	0.0	1.4632782994364915E-4	4.3898348983094745E-4
17	0.0	0.0	0.0	2.926556598872983E-4	4.3898348983094745E-4
18	0.0	0.0	0.0	4.3898348983094745E-4	7.316391497182458E-4
19	0.0	0.0	0.0	4.3898348983094745E-4	7.316391497182458E-4
20	0.0	0.0	0.0	4.3898348983094745E-4	7.316391497182458E-4
21	0.0	0.0	0.0	7.316391497182458E-4	7.316391497182458E-4
22	0.0	0.0	0.0	0.0014632782994364916	8.779669796618949E-4
23	0.0	0.0	0.0	0.0021949174491547375	0.001024294809605544
24	0.0	0.0	0.0	0.0032192122587602814	0.001024294809605544
25	0.0	0.0	0.0	0.003950851408478527	0.001024294809605544
26	0.0	0.0	0.0	0.004828818388140422	0.001024294809605544
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCCGT	35	0.0020633005	22.86169	6
GCTATAC	45	3.5317393E-4	21.336018	1
ATCGTTT	170	0.0	20.703941	29
CGGCATC	175	0.0	19.1982	25
GGCATCG	175	0.0	19.1982	26
CTAGCGG	245	0.0	18.937	29
TCTAGCG	250	0.0	18.55826	28
AGCGGCG	250	0.0	18.55826	31
GAGCGTT	70	1.7813554E-5	18.290691	7
CATCGTT	185	0.0	18.160458	28
TAGCGGC	260	0.0	17.84448	30
TATAAGG	45	0.0088402955	17.780014	2
TCGCCAA	45	0.008851483	17.776112	22
TCGTTTA	190	0.0	17.682552	30
TCAATAC	55	0.0013620609	17.45802	3
CCCATAT	55	0.0013627292	17.45674	1
TAAGAGG	350	0.0	17.374886	4
GATATAT	75	3.2271062E-5	17.068813	1
GCATTCG	350	0.0	16.4556	22
CGTTTAT	205	0.0	16.388706	31
>>END_MODULE