FastQCFastQC Report
Thu 2 Feb 2017
SRR4062901_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062901_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819055
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT18210.22232939179908556No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12850.15688812106635086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12530.15298117953006818No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10470.1278302433902485No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9660.11794079762653302No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9610.11733033801148884No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8960.10939436301591468No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8690.10609788109467617No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT8650.10560951340264084No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8250.1007258364822875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2250.022.7529528
CTAGCGG2300.022.2583229
TAGCGGC2400.021.3308930
TATAGGT604.7250087E-621.3295884
AGCGGCG2700.019.55331631
TAAGACC902.0403604E-819.5521244
TTAGACC507.202021E-419.1966324
GCGGCGC2850.019.08553332
ATTAGAC601.1386743E-418.664533
CAAGACG2500.018.5567424
CGTCGTA1900.018.52645710
ATACCGT2250.018.4867736
TCTTGCG2950.018.4498312
GCCGGTC3400.018.3508428
AAGACGG2650.018.110035
CGCCGGT3100.018.0624497
CGGAATG802.7972474E-617.9979428
CGCAAGA2600.017.8550112
CTTGCGC3050.017.8340243
TCGAGTG450.00884777217.77791412
Produced by FastQC (version 0.11.5)