##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062901_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819055
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221830035833978	32.0	32.0	32.0	32.0	32.0
2	30.800347961980574	32.0	32.0	32.0	32.0	32.0
3	30.851428780729012	32.0	32.0	32.0	32.0	32.0
4	30.92602084109126	32.0	32.0	32.0	32.0	32.0
5	30.808220449176186	32.0	32.0	32.0	32.0	32.0
6	34.496193784300196	36.0	36.0	36.0	32.0	36.0
7	34.415520325252885	36.0	36.0	36.0	32.0	36.0
8	34.37746549377026	36.0	36.0	36.0	32.0	36.0
9	34.56170342650982	36.0	36.0	36.0	32.0	36.0
10	34.22362356618298	36.0	36.0	36.0	32.0	36.0
11	34.547783726367584	36.0	36.0	36.0	32.0	36.0
12	34.343089291927896	36.0	36.0	36.0	32.0	36.0
13	34.44482604953269	36.0	36.0	36.0	32.0	36.0
14	34.328505411724485	36.0	36.0	36.0	32.0	36.0
15	34.27495711521205	36.0	36.0	36.0	32.0	36.0
16	34.28419214826843	36.0	36.0	36.0	32.0	36.0
17	34.20524506901246	36.0	36.0	36.0	32.0	36.0
18	34.23845651390932	36.0	36.0	36.0	32.0	36.0
19	34.21580724127195	36.0	36.0	36.0	32.0	36.0
20	34.200494472288185	36.0	36.0	36.0	32.0	36.0
21	34.192852738827064	36.0	36.0	36.0	32.0	36.0
22	34.160306694910595	36.0	36.0	36.0	32.0	36.0
23	34.09083028612242	36.0	36.0	36.0	32.0	36.0
24	34.077669997741296	36.0	36.0	36.0	32.0	36.0
25	34.045769820097554	36.0	36.0	36.0	32.0	36.0
26	33.9984579790124	36.0	36.0	36.0	32.0	36.0
27	33.9989329165929	36.0	36.0	36.0	32.0	36.0
28	33.96753209491426	36.0	36.0	36.0	32.0	36.0
29	33.91906038056052	36.0	36.0	36.0	32.0	36.0
30	33.88966919193461	36.0	36.0	36.0	32.0	36.0
31	33.90397958623047	36.0	36.0	36.0	32.0	36.0
32	33.84944844973781	36.0	36.0	36.0	32.0	36.0
33	33.82784916763831	36.0	36.0	36.0	32.0	36.0
34	33.8217091648302	36.0	36.0	36.0	32.0	36.0
35	33.79511876491811	36.0	36.0	36.0	32.0	36.0
36	33.72557886832997	36.0	36.0	36.0	27.0	36.0
37	33.71534512334337	36.0	36.0	36.0	27.0	36.0
38	33.10313837288094	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	3.0
10	9.0
11	9.0
12	6.0
13	2.0
14	55.0
15	159.0
16	244.0
17	270.0
18	405.0
19	541.0
20	739.0
21	1043.0
22	1575.0
23	2458.0
24	3769.0
25	5711.0
26	8328.0
27	12270.0
28	16781.0
29	22856.0
30	31020.0
31	41060.0
32	56205.0
33	82639.0
34	179097.0
35	351796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.842996069706174	17.244637868961682	11.232205182119914	25.680160879212227
2	16.175126804335292	19.372110864614996	38.807379572530124	25.64538275851959
3	19.388075320193856	23.822740388078984	28.306980708388842	28.48220358333832
4	12.130154757180794	15.079637373706541	36.669210341564664	36.120997527548
5	13.998849891276876	37.253999435933935	34.13258971031179	14.614560962477396
6	34.22086238619479	35.90048507239458	16.92820855304846	12.95044398836217
7	30.10924798609127	30.346228697985726	20.982865598605223	18.561657717317782
8	27.785171149084555	34.35154300337464	19.32099549234969	18.542290355191124
9	27.37475184786351	14.094064616008223	18.654569784165634	39.87661375196263
10	15.574488035543574	27.02261519734424	32.194942683666824	25.207954083445355
11	37.82689224811028	20.916098012426758	22.46400965033411	18.793000089128846
12	24.783645980357207	23.705660856527494	29.40778581440439	22.10290734871091
13	29.957090244807848	19.653864564406152	25.334095305081767	25.054949885704236
14	22.810607790393693	20.373194559594033	25.15375777030781	31.66243987970446
15	24.677142218623718	28.586850727658852	22.398117380403708	24.33788967331372
16	24.996275791669007	25.893382631269183	25.237189333867338	23.87315224319447
17	23.43632271607682	26.553846867760754	25.94948997847078	24.060340437691647
18	24.346041327843793	24.89804789185018	27.78652815831758	22.969382621988444
19	25.279581144161185	25.221583090453763	25.508887133345464	23.98994863203959
20	25.30915901909167	24.836507138008	25.997562869659923	23.85677097324041
21	26.229882503195967	24.62316895827966	24.480434089824065	24.66651444870031
22	25.254306202861322	25.63037779321679	25.17909188479022	23.936224119131666
23	23.520067206122793	25.18788775698344	26.202206910362126	25.089838126531642
24	24.585293661541016	25.593288015307348	25.805998634842293	24.015419688309343
25	24.341473005002985	25.323631017290033	25.931633596324875	24.403262381382103
26	23.512847159044423	26.32370127134426	26.5392350930581	23.624216476553215
27	24.582555426260512	25.768433802906333	25.612140112409353	24.036870658423805
28	23.535839961318775	25.59163469686277	26.067091411254182	24.805433930564273
29	23.86820421898887	25.489154590433767	25.962435467248866	24.6802057233285
30	23.75983639083056	25.711913555752265	26.541070174903087	23.987179878514088
31	24.75012271032305	25.240964002529907	25.255860181049616	24.753053106097422
32	23.95558990626408	25.958390872805104	25.33812452457439	24.747894696356422
33	23.350675895954392	25.560356879901637	26.386499419404704	24.70246780473927
34	24.36127409379255	25.826469744241	26.36240971505675	23.449846446909707
35	24.83068957516699	25.51879937966321	26.255632487086494	23.394878558083306
36	23.3563344148034	26.245166048596552	26.047473157603605	24.351026378996444
37	24.60446834464645	25.933835677156786	25.252118232736358	24.209577745460408
38	23.811593707417376	26.29829693917919	25.540539880536656	24.34956947286678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	67.0
2	88.0
3	88.0
4	216.5
5	345.0
6	345.0
7	476.0
8	607.0
9	634.5
10	662.0
11	662.0
12	869.0
13	1076.0
14	1465.0
15	1854.0
16	1854.0
17	2606.0
18	3358.0
19	3358.0
20	4082.0
21	4806.0
22	5555.0
23	6304.0
24	6304.0
25	7968.0
26	9632.0
27	9632.0
28	12794.5
29	15957.0
30	20229.5
31	24502.0
32	24502.0
33	30775.5
34	37049.0
35	37049.0
36	40375.0
37	43701.0
38	48295.0
39	52889.0
40	52889.0
41	54262.0
42	55635.0
43	59869.0
44	64103.0
45	64103.0
46	66655.0
47	69207.0
48	69207.0
49	70960.0
50	72713.0
51	71754.0
52	70795.0
53	70795.0
54	66838.0
55	62881.0
56	62881.0
57	60553.5
58	58226.0
59	52396.5
60	46567.0
61	46567.0
62	43517.5
63	40468.0
64	33844.0
65	27220.0
66	27220.0
67	22956.0
68	18692.0
69	18692.0
70	15140.5
71	11589.0
72	9037.5
73	6486.0
74	6486.0
75	4918.5
76	3351.0
77	3351.0
78	3167.0
79	2983.0
80	2348.0
81	1713.0
82	1713.0
83	1704.0
84	1695.0
85	1695.0
86	1144.0
87	593.0
88	506.5
89	420.0
90	420.0
91	291.5
92	163.0
93	154.5
94	146.0
95	146.0
96	130.0
97	114.0
98	114.0
99	266.5
100	419.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12819651915927502
2	0.05750529573716051
3	0.011965008454865669
4	0.0036627576902650005
5	2.441838460176667E-4
6	2.441838460176667E-4
7	1.2209192300883335E-4
8	3.6627576902650003E-4
9	1.2209192300883335E-4
10	4.883676920353334E-4
11	0.0019534707681413336
12	0.0032964819212385004
13	0.015505674222121837
14	0.008790618456636
15	0.023930016909731337
16	0.010866181147786168
17	0.020511443065484
18	0.005249952689379834
19	0.007569699226547668
20	0.007203423457521168
21	0.006592963842477001
22	0.007569699226547668
23	0.010011537686724335
24	0.012453376146901002
25	0.017581236913272
26	0.01929052383539567
27	0.010255721532742
28	0.006470871919468168
29	0.011110364993803835
30	0.0036627576902650005
31	0.006470871919468168
32	0.006348779996459334
33	0.008302250764600668
34	0.014651030761060002
35	0.017703328836280834
36	0.013185927684954002
37	0.011476640762830335
38	0.007203423457521168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	819055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.614918691739916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95206431932684	48.2595073232517
2	12.243274399227182	14.597636156611788
3	3.342200184039734	5.977349766691409
4	1.2925006364078144	3.0820928139389587
5	0.5948917314678666	1.7732211100922621
6	0.35785341581677105	1.2800041372486919
7	0.19980963727710818	0.8338144696070594
8	0.15172502165133833	0.723605986339761
9	0.10460443721405316	0.5612386517379893
>10	0.5942727023676425	6.7946642299995155
>50	0.08010779954037625	3.3393584363945537
>100	0.07825266497713837	9.049414357083146
>500	0.007619338424116645	3.0644311942756284
>1k	8.237122620666645E-4	0.6636613667275878
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1821	0.22232939179908556	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1285	0.15688812106635086	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1253	0.15298117953006818	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1047	0.1278302433902485	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	966	0.11794079762653302	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	961	0.11733033801148884	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	896	0.10939436301591468	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	869	0.10609788109467617	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	865	0.10560951340264084	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	825	0.1007258364822875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.2209192300883335E-4	0.0
18	0.0	0.0	0.0	1.2209192300883335E-4	0.0
19	0.0	0.0	0.0	2.441838460176667E-4	0.0
20	0.0	0.0	0.0	2.441838460176667E-4	0.0
21	0.0	0.0	0.0	4.883676920353334E-4	0.0
22	0.0	0.0	0.0	6.104596150441668E-4	0.0
23	0.0	0.0	0.0	0.0015871949991148335	0.0
24	0.0	0.0	0.0	0.002319746537167834	0.0
25	0.0	0.0	0.0	0.003174389998229667	0.0
26	0.0	0.0	0.0	0.003784849613273834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	225	0.0	22.75295	28
CTAGCGG	230	0.0	22.25832	29
TAGCGGC	240	0.0	21.33089	30
TATAGGT	60	4.7250087E-6	21.329588	4
AGCGGCG	270	0.0	19.553316	31
TAAGACC	90	2.0403604E-8	19.552124	4
TTAGACC	50	7.202021E-4	19.196632	4
GCGGCGC	285	0.0	19.085533	32
ATTAGAC	60	1.1386743E-4	18.66453	3
CAAGACG	250	0.0	18.556742	4
CGTCGTA	190	0.0	18.526457	10
ATACCGT	225	0.0	18.486773	6
TCTTGCG	295	0.0	18.449831	2
GCCGGTC	340	0.0	18.350842	8
AAGACGG	265	0.0	18.11003	5
CGCCGGT	310	0.0	18.062449	7
CGGAATG	80	2.7972474E-6	17.99794	28
CGCAAGA	260	0.0	17.855011	2
CTTGCGC	305	0.0	17.834024	3
TCGAGTG	45	0.008847772	17.777914	12
>>END_MODULE