FastQCFastQC Report
Thu 2 Feb 2017
SRR4062901_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062901_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819055
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20220.24686986832386104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15230.18594599874245318No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12490.15249281183803287No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12060.147242859148653No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11630.14199290645927318No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10070.12294656646989519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9200.11232456916812669No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9080.11085946609202069No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9060.11061528224600302No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9010.11000482263095884No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT8430.10292349109644651No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8330.10170257186635817No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8270.10097002032830517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1051.2732926E-1121.33304816
ACGAACG1102.3646862E-1120.36336515
GTCGTTG400.004482744319.99973315
CACGTTT2650.019.92426128
TCACGTT2500.019.83973327
CGCCGGT1950.019.6932457
GTCTATA601.1372585E-418.6675591
CGGTCCA2100.018.28547110
TAGCGGC2050.017.9509830
CTAGCGG2050.017.9509829
TAACGAA1251.4733814E-1017.9197613
CTAGGAC1006.803384E-817.6008423
GTCCTAC5750.017.5312711
ATCGTTT1650.017.45431129
CGTTTTT2850.017.40327630
ATACCGT1850.017.298126
TAATACC652.0987105E-417.231594
ATAACGA1302.582965E-1017.23053712
TCTAGCG2150.017.1160528
ACGTTTT2900.017.1032229
Produced by FastQC (version 0.11.5)