##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062901_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819055
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206304826904177	32.0	32.0	32.0	32.0	32.0
2	31.293211078621095	32.0	32.0	32.0	32.0	32.0
3	31.385710361331046	32.0	32.0	32.0	32.0	32.0
4	31.48985599257681	32.0	32.0	32.0	32.0	32.0
5	31.413441099804043	32.0	32.0	32.0	32.0	32.0
6	34.95837764252706	36.0	36.0	36.0	36.0	36.0
7	34.98477635812003	36.0	36.0	36.0	36.0	36.0
8	34.9195340972218	36.0	36.0	36.0	36.0	36.0
9	35.03417719200786	36.0	36.0	36.0	36.0	36.0
10	34.879835908455476	36.0	36.0	36.0	32.0	36.0
11	35.06494679844455	36.0	36.0	36.0	36.0	36.0
12	34.95219612846512	36.0	36.0	36.0	36.0	36.0
13	35.012504654754565	36.0	36.0	36.0	36.0	36.0
14	34.95668300663569	36.0	36.0	36.0	32.0	36.0
15	34.91930822716423	36.0	36.0	36.0	32.0	36.0
16	34.932908046468185	36.0	36.0	36.0	36.0	36.0
17	34.901289901166585	36.0	36.0	36.0	32.0	36.0
18	34.90318843056937	36.0	36.0	36.0	32.0	36.0
19	34.90340941695002	36.0	36.0	36.0	32.0	36.0
20	34.882888206530694	36.0	36.0	36.0	32.0	36.0
21	34.863080012941744	36.0	36.0	36.0	32.0	36.0
22	34.84466488819432	36.0	36.0	36.0	32.0	36.0
23	34.78913137701375	36.0	36.0	36.0	32.0	36.0
24	34.77107276068152	36.0	36.0	36.0	32.0	36.0
25	34.74581316273022	36.0	36.0	36.0	32.0	36.0
26	34.68431301927221	36.0	36.0	36.0	32.0	36.0
27	34.65836360195591	36.0	36.0	36.0	32.0	36.0
28	34.633793823369615	36.0	36.0	36.0	32.0	36.0
29	34.58150307366416	36.0	36.0	36.0	32.0	36.0
30	34.55813223776181	36.0	36.0	36.0	32.0	36.0
31	34.54854069629024	36.0	36.0	36.0	32.0	36.0
32	34.521964947408904	36.0	36.0	36.0	32.0	36.0
33	34.493706771828506	36.0	36.0	36.0	32.0	36.0
34	34.48589044691749	36.0	36.0	36.0	32.0	36.0
35	34.444330356325274	36.0	36.0	36.0	32.0	36.0
36	34.41960430007753	36.0	36.0	36.0	32.0	36.0
37	34.38751976363004	36.0	36.0	36.0	32.0	36.0
38	33.9579857274542	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	1.0
21	16.0
22	36.0
23	162.0
24	410.0
25	1161.0
26	2511.0
27	4928.0
28	8796.0
29	14530.0
30	22155.0
31	32412.0
32	47431.0
33	76059.0
34	188213.0
35	420232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.20035556250183	18.34113975364598	11.989977826184639	26.468526857667552
2	15.197270024601522	20.377019858251277	38.090482324141846	26.335227793005355
3	18.337474284388716	24.448419214826846	28.734700355897957	28.47940614488649
4	11.687399960686305	16.034615586537136	36.62595705039845	35.65202740237811
5	13.424129026744236	37.67695698091093	33.9420429641477	14.95687102819713
6	34.269905048647765	36.31690822671604	16.428891485389798	12.984295239246396
7	29.891324917861855	30.623761517959284	20.912719294497144	18.572194269681717
8	27.62904547191627	34.65806628060164	19.087631389699805	18.625256857782286
9	27.05083264043291	14.00537959344144	18.368027193441925	40.57576057268373
10	15.305940555376484	27.188028052079975	32.31104892509352	25.194982467450018
11	37.94535367003169	20.802991897554616	22.500158727833046	18.75149570458064
12	24.561474193914666	23.91438384178763	29.290483742770597	22.233658221527108
13	29.872865059684926	19.72685461589083	25.241651618763665	25.158628705660576
14	22.728632387324417	20.36945015902473	25.092576200621448	31.809341253029405
15	24.567336747837444	28.733723620513885	22.295816520258104	24.403123111390563
16	25.064159383874568	26.00121603703785	25.007386570360442	23.92723800872714
17	23.411492512712822	26.715910408946897	25.79436057407622	24.078236504264062
18	24.305205389137484	24.950461202239165	27.6489368845804	23.09539652404295
19	25.200993828253292	25.22883078669931	25.5590894384382	24.0110859466092
20	25.332364737410796	24.75230601119583	26.026823595485038	23.888505655908336
21	26.167383347129736	24.711435387654536	24.474088399543863	24.64709286567186
22	25.245771941219846	25.574322509437742	25.335998202800308	23.8439073465421
23	23.42406798078273	25.085128593317908	26.25086227420625	25.239941151693106
24	24.466000451740115	25.544438407677138	25.836970655206304	24.15259048537644
25	24.407518420618885	25.219551800550633	25.87689471403019	24.49603506480029
26	23.445800343078304	26.3703902668319	26.47734279138764	23.706466598702164
27	24.52463451738225	25.63371890009694	25.614916720997638	24.22672986152317
28	23.555927257632273	25.533450134606344	26.08872420045052	24.821898407310865
29	23.902912502823376	25.266068823217005	26.154043379260244	24.67697529469938
30	23.70402476024199	25.510741036926703	26.580754650176118	24.204479552655194
31	24.719463283906453	25.17584289211347	25.25031896514886	24.854374858831214
32	24.06163200273486	25.719151949502784	25.424422047359457	24.794794000402902
33	23.40831812271459	25.467398404258567	26.465011507163744	24.6592719658631
34	24.251362851090587	25.59412982034174	26.572208215565503	23.58229911300218
35	24.783439451563083	25.459218245416977	26.201415045387673	23.555927257632273
36	23.415155270403087	26.011440013185926	26.150014345800955	24.423390370610033
37	24.65072553125248	25.806813950223123	25.28694654205151	24.255513976472887
38	23.962644709628425	25.96165820568607	25.58414951883514	24.491547565850357
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	35.0
2	50.0
3	50.0
4	164.0
5	278.0
6	278.0
7	401.5
8	525.0
9	616.5
10	708.0
11	708.0
12	897.0
13	1086.0
14	1441.0
15	1796.0
16	1796.0
17	2704.5
18	3613.0
19	3613.0
20	4211.0
21	4809.0
22	5597.0
23	6385.0
24	6385.0
25	8012.5
26	9640.0
27	9640.0
28	12888.5
29	16137.0
30	20275.5
31	24414.0
32	24414.0
33	30908.5
34	37403.0
35	37403.0
36	40448.5
37	43494.0
38	48139.5
39	52785.0
40	52785.0
41	53748.0
42	54711.0
43	59483.5
44	64256.0
45	64256.0
46	67049.0
47	69842.0
48	69842.0
49	71689.5
50	73537.0
51	72299.5
52	71062.0
53	71062.0
54	66990.0
55	62918.0
56	62918.0
57	60772.5
58	58627.0
59	52305.0
60	45983.0
61	45983.0
62	43342.5
63	40702.0
64	33943.0
65	27184.0
66	27184.0
67	23061.0
68	18938.0
69	18938.0
70	15215.5
71	11493.0
72	9015.5
73	6538.0
74	6538.0
75	4920.5
76	3303.0
77	3303.0
78	3115.0
79	2927.0
80	2303.0
81	1679.0
82	1679.0
83	1560.0
84	1441.0
85	1441.0
86	929.5
87	418.0
88	338.5
89	259.0
90	259.0
91	160.5
92	62.0
93	39.0
94	16.0
95	16.0
96	13.0
97	10.0
98	10.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008668526533627168
2	0.0
3	0.0
4	2.441838460176667E-4
5	0.0
6	4.883676920353334E-4
7	9.767353840706668E-4
8	0.0026860223061943335
9	0.004151125382300334
10	8.546434610618334E-4
11	0.005249952689379834
12	4.883676920353334E-4
13	4.883676920353334E-4
14	0.0
15	0.0
16	1.2209192300883335E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2209192300883335E-4
22	3.6627576902650003E-4
23	0.0
24	0.0
25	0.0
26	0.0
27	1.2209192300883335E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.2209192300883335E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	819055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.4317093637095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3368947084376	45.564534787142115
2	13.244522049510362	15.213110820174428
3	3.6466315439106474	6.282968489592347
4	1.4305881710807085	3.2864449624267196
5	0.6499963466607857	1.8665200634447605
6	0.3724280840241274	1.2833508888334122
7	0.23018805365166944	0.9254065377424456
8	0.15253075609536446	0.7008081642476667
9	0.11818854231665527	0.6108993011205566
>10	0.640282969253476	7.12721326441316
>50	0.08342077416268404	3.4068771138435565
>100	0.08491659038031865	9.609468230433267
>500	0.008128036354427427	3.118762280134557
>1k	0.0012833741612253832	1.003635096451053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2022	0.24686986832386104	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1523	0.18594599874245318	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1249	0.15249281183803287	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1206	0.147242859148653	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1163	0.14199290645927318	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1007	0.12294656646989519	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	920	0.11232456916812669	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	908	0.11085946609202069	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	906	0.11061528224600302	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	901	0.11000482263095884	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	843	0.10292349109644651	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	833	0.10170257186635817	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	827	0.10097002032830517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	1.2209192300883335E-4	0.0
18	0.0	0.0	0.0	1.2209192300883335E-4	0.0
19	0.0	0.0	0.0	1.2209192300883335E-4	0.0
20	0.0	0.0	0.0	1.2209192300883335E-4	0.0
21	0.0	0.0	0.0	3.6627576902650003E-4	0.0
22	0.0	0.0	0.0	6.104596150441668E-4	0.0
23	0.0	0.0	0.0	0.0015871949991148335	0.0
24	0.0	0.0	0.0	0.0025639303831855005	0.0
25	0.0	0.0	0.0	0.0030522980752208336	0.0
26	0.0	0.0	0.0	0.003784849613273834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	105	1.2732926E-11	21.333048	16
ACGAACG	110	2.3646862E-11	20.363365	15
GTCGTTG	40	0.0044827443	19.999733	15
CACGTTT	265	0.0	19.924261	28
TCACGTT	250	0.0	19.839733	27
CGCCGGT	195	0.0	19.693245	7
GTCTATA	60	1.1372585E-4	18.667559	1
CGGTCCA	210	0.0	18.285471	10
TAGCGGC	205	0.0	17.95098	30
CTAGCGG	205	0.0	17.95098	29
TAACGAA	125	1.4733814E-10	17.91976	13
CTAGGAC	100	6.803384E-8	17.600842	3
GTCCTAC	575	0.0	17.531271	1
ATCGTTT	165	0.0	17.454311	29
CGTTTTT	285	0.0	17.403276	30
ATACCGT	185	0.0	17.29812	6
TAATACC	65	2.0987105E-4	17.23159	4
ATAACGA	130	2.582965E-10	17.230537	12
TCTAGCG	215	0.0	17.11605	28
ACGTTTT	290	0.0	17.10322	29
>>END_MODULE