FastQCFastQC Report
Thu 2 Feb 2017
SRR4062900_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062900_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143301
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53130.24788865399680213No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34350.1602668034027885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34100.15910037834163285No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32420.15126200193066677No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT27750.1294731817882789No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27060.1262538486194893No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26140.12196140439443644No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25710.11995515328924869No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25410.1185554432158619No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25160.11738901815470622No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG23820.11113697982691187No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC23130.1079176466581222No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22900.106844535601859No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22540.10516488351379484No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22000.1026454053816986No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG21770.1015722943254354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6450.017.61155528
ATCGTTT6050.017.19045829
CTAGCGG6650.017.0830829
ATACCGT5000.016.9560186
CATCGTT6150.016.90975628
TACCGTC5150.016.4644587
ACCGTCG5200.016.3065268
GTCCTAC5950.016.151391
TATACCG703.704878E-415.99624355
TAGCGGC7150.015.88845930
TCGTTTA6350.015.87436330
GTATCAA42800.015.4928811
GCATCGT6900.015.30361227
CGAACGA2950.015.186419516
CCGTCGT5600.015.1431889
AAGACGG8100.015.00882155
ACGAACG3100.014.96806915
CGGTCCA8150.014.92057210
GTTATTC5200.014.7667973
CGTCGTA5650.014.72598610
Produced by FastQC (version 0.11.5)