FastQCFastQC Report
Thu 2 Feb 2017
SRR4062900_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062900_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143301
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT61090.2850276279439985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT42570.19861885941358678No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34130.1592403493489715No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34060.15891375033184793No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG28420.1325992009521761No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28100.13110617687389686No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC28060.13091954886411195No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT27480.12821344272223079No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC27280.12728030267330626No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25860.12065500832594209No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25550.11920864125010905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24630.1149161970250562No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24540.11449628400304016No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23940.11169686385626656No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC21610.10082578228629577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6800.018.58789828
CTAGCGG6850.018.45221729
TAGCGGC7400.017.08076930
TAGGACG5700.016.5618744
CGCGCCA1551.0913936E-1116.5158310
AGGACGT6250.016.1284585
GTTATTC4600.016.0004563
AAGACGG8400.016.0004545
CGTTATT4500.015.6445242
CAAGACG8750.015.5433014
GGACGTG6200.015.4843116
TAGACTG2600.015.3850545
GTAGGAC6400.014.750423
AGCGGCG8700.014.71237631
GTATCAA41900.014.7027751
TTAGGAC4700.014.6387143
GTATTAG5300.014.4916551
GTCTTAC1553.1341187E-914.4526981
GCGCAAG9000.014.4010821
CGCAAGA9250.014.3568292
Produced by FastQC (version 0.11.5)