##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062900_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2143301
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239559912490126	32.0	32.0	32.0	32.0	32.0
2	31.332432075569415	32.0	32.0	32.0	32.0	32.0
3	31.40389147394603	32.0	32.0	32.0	32.0	32.0
4	31.491295902908643	32.0	32.0	32.0	32.0	32.0
5	31.41526318515225	32.0	32.0	32.0	32.0	32.0
6	34.96268792857373	36.0	36.0	36.0	36.0	36.0
7	34.98473289565955	36.0	36.0	36.0	36.0	36.0
8	34.92482250509845	36.0	36.0	36.0	36.0	36.0
9	35.03166564099023	36.0	36.0	36.0	36.0	36.0
10	34.873964972721986	36.0	36.0	36.0	32.0	36.0
11	35.047992792426264	36.0	36.0	36.0	36.0	36.0
12	34.94569964741303	36.0	36.0	36.0	36.0	36.0
13	34.99932347346453	36.0	36.0	36.0	36.0	36.0
14	34.9420389390011	36.0	36.0	36.0	32.0	36.0
15	34.913945358118156	36.0	36.0	36.0	32.0	36.0
16	34.931211248443404	36.0	36.0	36.0	32.0	36.0
17	34.88996272572074	36.0	36.0	36.0	32.0	36.0
18	34.89639859263818	36.0	36.0	36.0	32.0	36.0
19	34.88768026516107	36.0	36.0	36.0	32.0	36.0
20	34.87387819069743	36.0	36.0	36.0	32.0	36.0
21	34.8623478456829	36.0	36.0	36.0	32.0	36.0
22	34.848884501057015	36.0	36.0	36.0	32.0	36.0
23	34.79312705028365	36.0	36.0	36.0	32.0	36.0
24	34.769917524416776	36.0	36.0	36.0	32.0	36.0
25	34.74781376950788	36.0	36.0	36.0	32.0	36.0
26	34.68567364079987	36.0	36.0	36.0	32.0	36.0
27	34.6776215753177	36.0	36.0	36.0	32.0	36.0
28	34.649662833171824	36.0	36.0	36.0	32.0	36.0
29	34.61598160967591	36.0	36.0	36.0	32.0	36.0
30	34.596076799292305	36.0	36.0	36.0	32.0	36.0
31	34.584292640184465	36.0	36.0	36.0	32.0	36.0
32	34.55353121190164	36.0	36.0	36.0	32.0	36.0
33	34.53190009242752	36.0	36.0	36.0	32.0	36.0
34	34.51680655213617	36.0	36.0	36.0	32.0	36.0
35	34.4884684885604	36.0	36.0	36.0	32.0	36.0
36	34.46643798514534	36.0	36.0	36.0	32.0	36.0
37	34.46069824070441	36.0	36.0	36.0	32.0	36.0
38	34.02819622628833	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	7.0
21	42.0
22	133.0
23	412.0
24	1252.0
25	3208.0
26	6854.0
27	13557.0
28	23819.0
29	37822.0
30	57072.0
31	82773.0
32	120376.0
33	193482.0
34	481211.0
35	1121276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45261537025804	17.84037142457188	11.802342401194531	26.90467080397555
2	16.36611003307515	20.00418979881967	36.35187031592856	27.277829852176623
3	18.52408971021802	23.48046308008068	28.1392580883413	29.856189121359993
4	12.33361498663041	15.622286598637519	35.16670811371452	36.87739030101754
5	14.598562963654176	36.45336630429711	32.791723043904256	16.15634768814445
6	35.13121845979277	35.333290408790944	15.999656603660902	13.535834527755384
7	31.05949583188113	30.042229410537896	19.904231479317108	18.994043278263867
8	28.71716797511079	32.57237346133783	18.61171170246419	20.098746861087186
9	27.546962047759916	13.873704052780395	18.002444919326994	40.57688898013269
10	16.254262150979525	26.07843850838246	30.54466883530134	27.12263050533667
11	38.57931329883357	20.67083706023181	21.491851215213863	19.257998425720753
12	25.308543476962587	23.45630281584998	27.751929738122964	23.48322396906447
13	29.925903876131027	18.874472366638877	24.814059833984743	26.385563923245353
14	23.909334246566395	19.431661721802023	24.230241109391542	32.42876292224004
15	25.547881515475428	27.073285553452365	21.582642848577965	25.796190082494245
16	26.42841712705507	25.326657643193972	23.109701446228453	25.135223783522502
17	24.56061934371327	25.59990407320297	24.415236124090832	25.424240458992927
18	25.591505812762648	24.29971338603397	25.75233250019479	24.356448301008584
19	26.173691889286665	24.295840854830935	24.946239468931335	24.58422778695106
20	26.554039773228304	23.570884350821466	24.39396053097535	25.481115344974874
21	27.72246323189776	23.463792715711953	23.88669237782147	24.92705167456882
22	26.607160270218948	23.688482101657304	24.65134355962467	25.053014068499074
23	24.784293013440482	23.610822744915435	25.38794131108976	26.216942930554314
24	25.221749068376305	24.33162677570719	24.951511710207757	25.495112445708745
25	25.2800354593384	23.996687351280734	25.044230858955817	25.67904633042505
26	25.0727966559962	25.14684138797247	25.211554710751976	24.568807245279356
27	25.83778558194634	24.06430460705669	24.616210803998882	25.48169900699809
28	24.852738836029097	24.296540709867628	25.453820998543836	25.396899455559442
29	24.738055924016273	24.36909234867151	25.34688314893708	25.545968578375135
30	24.63298435450737	24.657899193813655	25.570230219647172	25.138886232031805
31	25.617913676147214	24.376044242036	24.246104490223257	25.75993759159353
32	25.413229406415617	24.36876574965439	24.199307516769693	26.0186973271603
33	24.795397380022685	23.990657401830166	25.049164816327714	26.16478040181944
34	25.69634409725932	24.2530097265853	24.996582374570814	25.054063801584565
35	26.11541729323133	24.015665555141343	25.13492038682388	24.73399676480345
36	24.704229597242758	24.831043329891603	24.811447388864185	25.653279684001458
37	26.07813834827679	24.584741013977972	24.338998582093694	24.99812205565154
38	25.004420750981783	24.332186659736546	24.941433797679373	25.7219587916023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	43.5
2	68.0
3	68.0
4	250.0
5	432.0
6	432.0
7	688.0
8	944.0
9	1150.5
10	1357.0
11	1357.0
12	1823.5
13	2290.0
14	3178.5
15	4067.0
16	4067.0
17	6314.0
18	8561.0
19	8561.0
20	10007.5
21	11454.0
22	11834.5
23	12215.0
24	12215.0
25	14538.0
26	16861.0
27	16861.0
28	22758.5
29	28656.0
30	36561.0
31	44466.0
32	44466.0
33	57001.0
34	69536.0
35	69536.0
36	78249.0
37	86962.0
38	100316.0
39	113670.0
40	113670.0
41	120469.5
42	127269.0
43	144416.5
44	161564.0
45	161564.0
46	164202.5
47	166841.0
48	166841.0
49	178523.0
50	190205.0
51	197197.5
52	204190.0
53	204190.0
54	196348.5
55	188507.0
56	188507.0
57	183486.5
58	178466.0
59	160256.0
60	142046.0
61	142046.0
62	135998.5
63	129951.0
64	110483.0
65	91015.0
66	91015.0
67	77511.5
68	64008.0
69	64008.0
70	51638.0
71	39268.0
72	31009.0
73	22750.0
74	22750.0
75	17523.5
76	12297.0
77	12297.0
78	11228.0
79	10159.0
80	7915.5
81	5672.0
82	5672.0
83	5271.0
84	4870.0
85	4870.0
86	3201.0
87	1532.0
88	1197.0
89	862.0
90	862.0
91	521.5
92	181.0
93	113.5
94	46.0
95	46.0
96	34.5
97	23.0
98	23.0
99	22.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009378057491691554
2	0.0
3	0.0
4	1.8662800978490654E-4
5	4.6657002446226636E-5
6	2.332850122311332E-4
7	3.732560195698131E-4
8	0.0020062511051877454
9	0.0037792171981443578
10	7.931690415858529E-4
11	0.004572386239730211
12	2.332850122311332E-4
13	1.8662800978490654E-4
14	0.0
15	0.0
16	9.331400489245327E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3997100733867992E-4
22	9.331400489245327E-5
23	0.0
24	0.0
25	4.6657002446226636E-5
26	9.331400489245327E-5
27	9.331400489245327E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2143301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.19704491599235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59138905474937	34.26099049731282
2	15.706588233644512	14.826091006806339
3	5.422365338432072	7.677588612866956
4	2.314134264951867	4.368811953782807
5	1.2011235256962236	2.834474049596988
6	0.7049059472446222	1.9961686592192742
7	0.40378460752003525	1.3340208180256614
8	0.2961468947240193	1.1181806633616957
9	0.20690725972776755	0.8788870107734705
>10	0.966155492482094	8.02917411619265
>50	0.0783473787205077	2.6165075867889227
>100	0.0846953312021967	8.593873750412822
>500	0.015604649712148814	5.077863664209528
>1k	0.007752628519411511	6.100791948504124
>5k	9.939267332578861E-5	0.28657566214600083
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6109	0.2850276279439985	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4257	0.19861885941358678	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3413	0.1592403493489715	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3406	0.15891375033184793	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2842	0.1325992009521761	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2810	0.13110617687389686	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2806	0.13091954886411195	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2748	0.12821344272223079	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2728	0.12728030267330626	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2586	0.12065500832594209	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2555	0.11920864125010905	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2463	0.1149161970250562	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2454	0.11449628400304016	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2394	0.11169686385626656	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2161	0.10082578228629577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	4.6657002446226636E-5	0.0	0.0	0.0	0.0
16	4.6657002446226636E-5	0.0	0.0	0.0	0.0
17	4.6657002446226636E-5	0.0	0.0	9.331400489245327E-5	0.0
18	4.6657002446226636E-5	0.0	0.0	1.3997100733867992E-4	0.0
19	4.6657002446226636E-5	0.0	0.0	1.3997100733867992E-4	0.0
20	4.6657002446226636E-5	0.0	0.0	1.8662800978490654E-4	0.0
21	4.6657002446226636E-5	0.0	0.0	2.332850122311332E-4	0.0
22	4.6657002446226636E-5	0.0	0.0	4.1991302201603976E-4	0.0
23	4.6657002446226636E-5	0.0	0.0	5.13227026908493E-4	0.0
24	4.6657002446226636E-5	0.0	0.0	7.465120391396262E-4	0.0
25	4.6657002446226636E-5	0.0	0.0	9.797970513707593E-4	0.0
26	4.6657002446226636E-5	0.0	0.0	0.0012597390660481192	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	680	0.0	18.587898	28
CTAGCGG	685	0.0	18.452217	29
TAGCGGC	740	0.0	17.080769	30
TAGGACG	570	0.0	16.561874	4
CGCGCCA	155	1.0913936E-11	16.51583	10
AGGACGT	625	0.0	16.128458	5
GTTATTC	460	0.0	16.000456	3
AAGACGG	840	0.0	16.000454	5
CGTTATT	450	0.0	15.644524	2
CAAGACG	875	0.0	15.543301	4
GGACGTG	620	0.0	15.484311	6
TAGACTG	260	0.0	15.385054	5
GTAGGAC	640	0.0	14.75042	3
AGCGGCG	870	0.0	14.712376	31
GTATCAA	4190	0.0	14.702775	1
TTAGGAC	470	0.0	14.638714	3
GTATTAG	530	0.0	14.491655	1
GTCTTAC	155	3.1341187E-9	14.452698	1
GCGCAAG	900	0.0	14.401082	1
CGCAAGA	925	0.0	14.356829	2
>>END_MODULE