FastQCFastQC Report
Thu 2 Feb 2017
SRR4062899_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062899_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823901
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33450.4059953805129499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21710.2635025324644587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12820.1556012190785058No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11530.13994399812598846No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10560.12817073896014206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.12210204866846866No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9870.11979594635763277No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9580.1162761059884622No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8930.1083868086092868No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8880.10777993958011944No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8680.10535246346345009No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8680.10535246346345009No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8620.1046242206284493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATT601.14023656E-418.6612154
AACCGCG1251.4733814E-1017.9202047
GGATAAT450.0088136417.789861
ATCGTGC450.00884762417.777988
ATCGTTT1550.017.54858629
CATTCCG550.001364096817.4547449
CGCGGTC1404.0017767E-1117.14305310
TACGCTA1900.016.8422979
CGGTCCT1456.91216E-1116.55191212
AGAACCG1651.8189894E-1216.4800345
ATACGCT2150.016.372288
GCCTTAA1001.303988E-616.0108741
GTGTTAC703.6757448E-416.0108721
CTCCTAT703.6757448E-416.0108721
TCCTATA908.554218E-616.0040682
AGGCCCG2700.016.00018110
CGAGCCG2500.016.00018115
TAACACC1001.3172721E-615.9953284
TTATACA1203.117202E-815.9953274
CATCGTT1753.6379788E-1215.54209128
Produced by FastQC (version 0.11.5)