##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062899_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	823901
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13776412457322	32.0	32.0	32.0	32.0	32.0
2	30.74092275649623	32.0	32.0	32.0	32.0	32.0
3	30.799131206297844	32.0	32.0	32.0	32.0	32.0
4	30.868722091610522	32.0	32.0	32.0	32.0	32.0
5	30.774707155349976	32.0	32.0	32.0	32.0	32.0
6	34.41245003950717	36.0	36.0	36.0	32.0	36.0
7	34.314975949780376	36.0	36.0	36.0	32.0	36.0
8	34.28601494597045	36.0	36.0	36.0	32.0	36.0
9	34.434610468976246	36.0	36.0	36.0	32.0	36.0
10	34.140211020498825	36.0	36.0	36.0	32.0	36.0
11	34.460944943627936	36.0	36.0	36.0	32.0	36.0
12	34.25038809274415	36.0	36.0	36.0	32.0	36.0
13	34.348208097817576	36.0	36.0	36.0	32.0	36.0
14	34.2473950146923	36.0	36.0	36.0	32.0	36.0
15	34.196715382066536	36.0	36.0	36.0	32.0	36.0
16	34.2124818394443	36.0	36.0	36.0	32.0	36.0
17	34.130123643496006	36.0	36.0	36.0	32.0	36.0
18	34.15804083257576	36.0	36.0	36.0	32.0	36.0
19	34.14386073084023	36.0	36.0	36.0	32.0	36.0
20	34.116251831227295	36.0	36.0	36.0	32.0	36.0
21	34.09709904466678	36.0	36.0	36.0	32.0	36.0
22	34.07419216629182	36.0	36.0	36.0	32.0	36.0
23	34.023524671046644	36.0	36.0	36.0	32.0	36.0
24	34.01843182615387	36.0	36.0	36.0	32.0	36.0
25	33.99071490385374	36.0	36.0	36.0	32.0	36.0
26	33.94889555905382	36.0	36.0	36.0	32.0	36.0
27	33.947871164132586	36.0	36.0	36.0	32.0	36.0
28	33.93724852864604	36.0	36.0	36.0	32.0	36.0
29	33.90314613042101	36.0	36.0	36.0	32.0	36.0
30	33.85730081648159	36.0	36.0	36.0	32.0	36.0
31	33.88914808939423	36.0	36.0	36.0	32.0	36.0
32	33.83484059371211	36.0	36.0	36.0	32.0	36.0
33	33.783999533924586	36.0	36.0	36.0	32.0	36.0
34	33.78541475250061	36.0	36.0	36.0	32.0	36.0
35	33.74197870860698	36.0	36.0	36.0	32.0	36.0
36	33.69491237418088	36.0	36.0	36.0	27.0	36.0
37	33.688557241707436	36.0	36.0	36.0	27.0	36.0
38	33.07218342980528	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	9.0
11	7.0
12	7.0
13	3.0
14	104.0
15	220.0
16	312.0
17	370.0
18	477.0
19	583.0
20	855.0
21	1258.0
22	1794.0
23	2703.0
24	4062.0
25	6053.0
26	8844.0
27	12547.0
28	17672.0
29	23805.0
30	31675.0
31	42391.0
32	57595.0
33	83966.0
34	181086.0
35	345498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86485717965979	17.13163580509453	11.413232359810529	25.590274655435145
2	16.445679393169513	20.200479460206502	37.777650936453306	25.57619021017068
3	18.82601143228936	24.978059181337375	28.40531823594604	27.790611150427225
4	12.395357051390201	15.7964880509496	36.26821124129568	35.539943656364514
5	14.360584584798417	36.807213488028296	33.48666890803628	15.345533019137008
6	33.22191190910536	35.85500164461906	17.401526157939262	13.521560288336312
7	29.673589423972054	30.881380165820893	21.278527395888585	18.16650301431847
8	28.07856302774976	32.43868483879699	19.93363264186508	19.54911949158817
9	28.042879042212697	14.290465214789677	18.707754227156485	38.95890151584114
10	15.965000436948355	26.634186839115614	31.620617165273284	25.780195558662744
11	36.77928351651019	21.7478167462692	22.29279571784897	19.180104019371637
12	25.027067180012523	24.141497021396425	28.70346993965111	22.127965858939938
13	29.776252027230438	19.76901712099288	25.163149563477806	25.291581288298875
14	23.88747070055267	19.968658240078852	25.60070694968931	30.543164109679168
15	25.139371130265875	26.755007891222533	23.314677673910406	24.79094330460119
16	25.411565344267945	25.977960830583903	24.11708581874859	24.493388006399567
17	23.810552819333683	25.768249763573436	25.516708893645074	24.90448852344781
18	24.706927650153304	24.844936870188793	26.81190205108162	23.636233428576283
19	25.288557594384343	25.16668912251059	26.0822806397852	23.462472643319874
20	25.633755290650463	23.983837040076033	25.87226697772313	24.510140691550376
21	27.097871328049987	24.284606243635043	24.79101095227633	23.826511476038633
22	25.72256054262898	24.395241314768263	25.3257606746933	24.556437467909465
23	24.00742880726372	24.400839989318055	25.955427156417667	25.636304047000557
24	24.35723442067338	25.342808308974195	25.73380886251736	24.566148407835065
25	24.690108102530488	24.64203555668858	25.728038069571657	24.93981827120928
26	24.28048034340766	25.440913681920595	26.234121280397794	24.044484694273947
27	25.139198252055962	25.16007647255182	25.23497101933056	24.465754256061665
28	24.289527379277615	24.924713510784084	26.20346105433858	24.582298055599725
29	24.145277098342447	25.380156711843217	26.22003993663549	24.254526253178845
30	24.16662620770015	25.427125309511094	26.34631256979172	24.059935912997037
31	24.512410056223963	25.395097153843913	25.11798292888979	24.97450986104233
32	24.230197803478312	25.35322049698488	25.113855954010933	25.302725745525873
33	23.958952895850377	24.881104688565898	25.83190302900827	25.32803938657545
34	24.64408664280963	25.13364959286037	25.878858358137208	24.343405406192794
35	25.504570450489823	24.83290238780242	25.797977590832392	23.864549570875365
36	24.230770631443626	25.81177718781372	25.394553636351496	24.562898544391153
37	25.101113830138477	25.72600458350327	25.24058536774845	23.9322962186098
38	24.128634910924426	25.549401530131057	25.708046722040756	24.61391683690376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	471.0
1	369.5
2	268.0
3	268.0
4	558.0
5	848.0
6	848.0
7	1057.5
8	1267.0
9	1208.5
10	1150.0
11	1150.0
12	1503.0
13	1856.0
14	2294.5
15	2733.0
16	2733.0
17	3832.0
18	4931.0
19	4931.0
20	5849.0
21	6767.0
22	6736.5
23	6706.0
24	6706.0
25	7707.5
26	8709.0
27	8709.0
28	11155.0
29	13601.0
30	16635.0
31	19669.0
32	19669.0
33	24344.0
34	29019.0
35	29019.0
36	32263.5
37	35508.0
38	40640.5
39	45773.0
40	45773.0
41	49230.5
42	52688.0
43	59220.0
44	65752.0
45	65752.0
46	69119.0
47	72486.0
48	72486.0
49	74905.5
50	77325.0
51	76430.5
52	75536.0
53	75536.0
54	71585.5
55	67635.0
56	67635.0
57	65815.5
58	63996.0
59	56751.5
60	49507.0
61	49507.0
62	46600.5
63	43694.0
64	35674.0
65	27654.0
66	27654.0
67	23067.0
68	18480.0
69	18480.0
70	14826.0
71	11172.0
72	8820.0
73	6468.0
74	6468.0
75	4969.0
76	3470.0
77	3470.0
78	3281.5
79	3093.0
80	2445.5
81	1798.0
82	1798.0
83	1839.5
84	1881.0
85	1881.0
86	1258.5
87	636.0
88	544.5
89	453.0
90	453.0
91	330.5
92	208.0
93	184.0
94	160.0
95	160.0
96	151.5
97	143.0
98	143.0
99	266.5
100	390.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11882495591096502
2	0.05765255777089723
3	0.011409137748345979
4	0.0033984665633371
5	0.0
6	2.4274761166693571E-4
7	0.0
8	2.4274761166693571E-4
9	2.4274761166693571E-4
10	6.068690291673392E-4
11	0.0019419808933354857
12	0.0030343451458366964
13	0.014200735282515739
14	0.009709904466677428
15	0.024396134972527038
16	0.011530511554179447
17	0.021604537438357278
18	0.005219073650839118
19	0.007525175961675008
20	0.00509769984500565
21	0.006554185515007264
22	0.007282428350008072
23	0.009831278272510896
24	0.014200735282515739
25	0.017720575651686308
26	0.019055687515854455
27	0.009224409243343556
28	0.005825942680006457
29	0.011651885360012914
30	0.002548849922502825
31	0.006432811709173797
32	0.006432811709173797
33	0.008132044990842347
34	0.012744249612514125
35	0.01589996856418429
36	0.014807604311683079
37	0.009345783049177025
38	0.006068690291673393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	823901.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.785110668188395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79912401572396	51.1575304054056
2	11.485458253878697	14.192606185815063
3	2.8489017752723416	5.280591344039999
4	1.038684335292905	2.567009064215433
5	0.507200272143713	1.5668712472667292
6	0.2702871448178481	1.0019832692855641
7	0.18503319271365762	0.8002607402371097
8	0.11867925950006704	0.5866088945784232
9	0.09034488876736074	0.5023772055716897
>10	0.5022618876958127	5.957228518926713
>50	0.07178722180773937	3.1499573328470607
>100	0.07494087748174805	9.376338420748166
>500	0.00611359789268225	2.6405716597744506
>1k	0.001183277011486887	1.220065711287969
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3345	0.4059953805129499	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2171	0.2635025324644587	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1282	0.1556012190785058	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1153	0.13994399812598846	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1056	0.12817073896014206	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1006	0.12210204866846866	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	987	0.11979594635763277	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	958	0.1162761059884622	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	893	0.1083868086092868	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	888	0.10777993958011944	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	868	0.10535246346345009	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	868	0.10535246346345009	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	862	0.1046242206284493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2137380583346786E-4	0.0
5	0.0	0.0	0.0	1.2137380583346786E-4	0.0
6	0.0	0.0	0.0	1.2137380583346786E-4	0.0
7	0.0	0.0	0.0	1.2137380583346786E-4	0.0
8	0.0	0.0	0.0	1.2137380583346786E-4	0.0
9	0.0	0.0	0.0	2.4274761166693571E-4	0.0
10	0.0	0.0	0.0	2.4274761166693571E-4	1.2137380583346786E-4
11	0.0	0.0	0.0	2.4274761166693571E-4	1.2137380583346786E-4
12	0.0	0.0	0.0	2.4274761166693571E-4	4.8549522333387143E-4
13	0.0	0.0	0.0	2.4274761166693571E-4	4.8549522333387143E-4
14	0.0	0.0	0.0	2.4274761166693571E-4	4.8549522333387143E-4
15	0.0	0.0	0.0	2.4274761166693571E-4	4.8549522333387143E-4
16	0.0	0.0	0.0	2.4274761166693571E-4	4.8549522333387143E-4
17	0.0	0.0	0.0	2.4274761166693571E-4	4.8549522333387143E-4
18	0.0	0.0	0.0	3.6412141750040357E-4	4.8549522333387143E-4
19	0.0	0.0	0.0	3.6412141750040357E-4	4.8549522333387143E-4
20	0.0	0.0	0.0	6.068690291673392E-4	4.8549522333387143E-4
21	0.0	0.0	0.0	7.282428350008071E-4	4.8549522333387143E-4
22	0.0	0.0	0.0	9.709904466677429E-4	4.8549522333387143E-4
23	0.0	0.0	0.0	0.0021847285050024213	4.8549522333387143E-4
24	0.0	0.0	0.0	0.0038839617866709714	4.8549522333387143E-4
25	0.0	0.0	0.0	0.004854952233338714	4.8549522333387143E-4
26	0.0	0.0	0.0	0.006068690291673393	4.8549522333387143E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATATT	60	1.14023656E-4	18.661215	4
AACCGCG	125	1.4733814E-10	17.920204	7
GGATAAT	45	0.00881364	17.78986	1
ATCGTGC	45	0.008847624	17.77798	8
ATCGTTT	155	0.0	17.548586	29
CATTCCG	55	0.0013640968	17.454744	9
CGCGGTC	140	4.0017767E-11	17.143053	10
TACGCTA	190	0.0	16.842297	9
CGGTCCT	145	6.91216E-11	16.551912	12
AGAACCG	165	1.8189894E-12	16.480034	5
ATACGCT	215	0.0	16.37228	8
GCCTTAA	100	1.303988E-6	16.010874	1
GTGTTAC	70	3.6757448E-4	16.010872	1
CTCCTAT	70	3.6757448E-4	16.010872	1
TCCTATA	90	8.554218E-6	16.004068	2
AGGCCCG	270	0.0	16.000181	10
CGAGCCG	250	0.0	16.000181	15
TAACACC	100	1.3172721E-6	15.995328	4
TTATACA	120	3.117202E-8	15.995327	4
CATCGTT	175	3.6379788E-12	15.542091	28
>>END_MODULE