FastQCFastQC Report
Thu 2 Feb 2017
SRR4062899_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062899_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823901
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37930.4603708455263436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27120.32916576142036486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17020.20657821752856229No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.14783329550516383No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12090.14674093125266263No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11620.14103636237848965No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10480.12719974851347432No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9320.11312038703679204No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9080.11020741569678881No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9040.10972192047345494No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8770.10644482771595132No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8670.10523108965761663No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8670.10523108965761663No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8410.10207537070594647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA451.23791815E-524.88785232
TTAACGG400.004483503319.99916623
TAAGGTA400.004483503319.9991664
CGTCTTA507.195591E-419.199215
GACGCCA507.195591E-419.199227
GTAATAC601.13804956E-418.6658883
TCTCGTC601.13804956E-418.66588812
TAGCGGC1500.018.13257830
TCTAGCG1500.018.13257828
CTAGCGG1500.018.13257829
TAACCCG550.001364582717.45381728
GGTTCGT652.100164E-417.2300518
TTAGGGT957.68925E-716.8414044
CGCCCGA1850.016.43174626
GCCATAT600.002440809316.0003031
ATCGTTT2100.015.99933329
CGGTCCT1407.421477E-1015.99933312
TCGTTTG805.6197656E-515.99933221
GTATTAA1255.1290044E-815.3602911
GTATTAG1807.2759576E-1215.1113981
Produced by FastQC (version 0.11.5)