FastQCFastQC Report
Thu 2 Feb 2017
SRR4062898_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062898_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901643
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29710.32950957307936735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19620.2176027540833789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10800.11978133252296087No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10130.1123504535608883No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9960.11046500666006391No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9610.10658320421719017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.10125958943839192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG2550.018.19541710
CGTCGTA1600.017.99934610
CGAGCCG2550.017.5679915
ATCGTTT2200.017.45487629
CATCGTT2300.017.39067328
ATAAGTG652.1021356E-417.2282315
AAGACGG2250.017.0641525
TTCCGTC1700.016.94056116
ATACCGT1700.016.938686
TACCGTC1900.016.8414927
GCATCGT2500.016.63939527
GTCCTAG1354.3655746E-1016.6085681
CCGTCAA1551.0913936E-1116.5146118
ACCGTCG1750.016.4565438
GTTCAAA3450.016.2475131
TCCGTCA1601.8189894E-1115.99941817
CCGTCGT1800.015.9994169
CGGCATC2550.015.68570425
CAAGACG2350.015.6572684
GGCGGGT1451.2187229E-915.4485732
Produced by FastQC (version 0.11.5)