##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062898_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901643
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.208523772712702	32.0	32.0	32.0	32.0	32.0
2	30.81536927586639	32.0	32.0	32.0	32.0	32.0
3	30.87053190675245	32.0	32.0	32.0	32.0	32.0
4	30.933559069387773	32.0	32.0	32.0	32.0	32.0
5	30.85443019021941	32.0	32.0	32.0	32.0	32.0
6	34.50405426537998	36.0	36.0	36.0	32.0	36.0
7	34.42357119170226	36.0	36.0	36.0	32.0	36.0
8	34.3990382002633	36.0	36.0	36.0	32.0	36.0
9	34.53418370685515	36.0	36.0	36.0	32.0	36.0
10	34.26652122846848	36.0	36.0	36.0	32.0	36.0
11	34.54107224256163	36.0	36.0	36.0	32.0	36.0
12	34.3467514304442	36.0	36.0	36.0	32.0	36.0
13	34.433597332869	36.0	36.0	36.0	32.0	36.0
14	34.333655338088356	36.0	36.0	36.0	32.0	36.0
15	34.27876110611406	36.0	36.0	36.0	32.0	36.0
16	34.298247754377286	36.0	36.0	36.0	32.0	36.0
17	34.22694680710658	36.0	36.0	36.0	32.0	36.0
18	34.2493747525351	36.0	36.0	36.0	32.0	36.0
19	34.24020260790579	36.0	36.0	36.0	32.0	36.0
20	34.20882100787119	36.0	36.0	36.0	32.0	36.0
21	34.19593342376085	36.0	36.0	36.0	32.0	36.0
22	34.17375945912074	36.0	36.0	36.0	32.0	36.0
23	34.11235378082012	36.0	36.0	36.0	32.0	36.0
24	34.097813657955534	36.0	36.0	36.0	32.0	36.0
25	34.07480344216059	36.0	36.0	36.0	32.0	36.0
26	34.05062314020072	36.0	36.0	36.0	32.0	36.0
27	34.03536654751382	36.0	36.0	36.0	32.0	36.0
28	34.01394232528839	36.0	36.0	36.0	32.0	36.0
29	33.973970850990916	36.0	36.0	36.0	32.0	36.0
30	33.93918546475712	36.0	36.0	36.0	32.0	36.0
31	33.96571813899737	36.0	36.0	36.0	32.0	36.0
32	33.91360771391781	36.0	36.0	36.0	32.0	36.0
33	33.86788119022717	36.0	36.0	36.0	32.0	36.0
34	33.86524710999808	36.0	36.0	36.0	32.0	36.0
35	33.82389815037659	36.0	36.0	36.0	32.0	36.0
36	33.78132586844239	36.0	36.0	36.0	32.0	36.0
37	33.77725441222302	36.0	36.0	36.0	32.0	36.0
38	33.184638487738496	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	7.0
11	9.0
12	6.0
13	3.0
14	108.0
15	247.0
16	317.0
17	373.0
18	467.0
19	588.0
20	843.0
21	1216.0
22	1868.0
23	2686.0
24	3987.0
25	6131.0
26	9104.0
27	13168.0
28	18341.0
29	24468.0
30	32849.0
31	44584.0
32	60112.0
33	88744.0
34	196200.0
35	395212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.68585581654102	17.146645257789313	11.30406491065014	25.863434015019525
2	16.54602331932517	19.917193868662064	37.62674209696374	25.910040715049032
3	19.14502326561625	23.946114725277162	28.44905135073679	28.4598106583698
4	12.595730993827742	15.435413119790597	35.96967663581462	35.99917925056704
5	14.577182518116945	36.49441796189619	33.52206308046875	15.406336439518123
6	33.875826272126346	35.569074131582454	16.927265871079367	13.627833725211836
7	30.085410744607344	30.56963787219554	20.8930807425999	18.45187064059722
8	28.109001375271724	32.90393061532319	19.58442393860077	19.40264407080431
9	27.757610845114627	14.193786662319038	18.577016794932792	39.471585697633536
10	16.145468257841266	26.534647974801473	31.588882480812742	25.73100128654452
11	37.28404317971844	21.223965120831565	22.312220020030455	19.179771679419538
12	25.196479234091452	23.77304457965755	28.5931354824971	22.437340703753904
13	29.8906966838523	19.514535322252886	25.010981356097652	25.58378663779716
14	23.73550437295148	19.935889259232557	25.08870685973834	31.239899508077624
15	25.256427038936085	27.268017563283063	22.645785677360095	24.829769720420757
16	25.614723186000905	25.64112234122794	24.12738590684426	24.6167685659269
17	23.774305339853214	25.74213931363761	25.59825968885615	24.88529565765302
18	24.801186338035354	24.60387001745789	26.82603555021196	23.768908094294797
19	25.53930860686513	24.801929077133135	25.674073367488525	23.984688948513206
20	25.77265502734586	24.22884231238056	25.4734089101696	24.52509375010398
21	26.89634213296273	24.035843303670955	24.49425515560825	24.573559407758065
22	25.92744587611428	24.438962764630006	25.001414169871193	24.632177189384517
23	24.286794947446882	24.344139739386222	25.77243545660652	25.596629856560376
24	24.688664354273175	25.03153011750168	25.545107757018986	24.734697771206154
25	24.733666112035497	24.51613976705491	25.69706045479756	25.05313366611204
26	24.183124714359877	25.38614107405123	26.16785211939424	24.262882092194648
27	25.00906736469662	25.018273374113363	25.182539638525487	24.79011962266453
28	24.108381738504487	25.023430801771536	25.936150649241174	24.932036810482803
29	24.325328600743166	25.01170206865953	25.745438411624427	24.91753091897288
30	24.232516096116413	25.070013253994222	26.219173372226507	24.47829727766286
31	24.935419575882136	24.889167902447788	25.06042110096729	25.114991420702783
32	24.27452602810834	25.230733055082005	25.020658109125836	25.474082807683818
33	24.054265096375893	24.788758327103487	25.828051829356212	25.328924747164404
34	24.757556699510065	25.0244301209804	25.853334974948726	24.364678204560807
35	25.348694295989155	24.98286201090176	25.712418996656705	23.95602469645238
36	24.304534901649326	25.54921194117928	25.330913713410368	24.81533944376103
37	25.096387199247538	25.451433717994792	25.00876247265887	24.443416610098804
38	24.37022790695611	25.480924937555177	25.402286611741843	24.746560543746867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	409.0
1	316.0
2	223.0
3	223.0
4	506.5
5	790.0
6	790.0
7	919.5
8	1049.0
9	1018.5
10	988.0
11	988.0
12	1262.5
13	1537.0
14	1993.5
15	2450.0
16	2450.0
17	3450.0
18	4450.0
19	4450.0
20	5357.5
21	6265.0
22	6384.0
23	6503.0
24	6503.0
25	7695.0
26	8887.0
27	8887.0
28	11661.5
29	14436.0
30	18173.0
31	21910.0
32	21910.0
33	27478.0
34	33046.0
35	33046.0
36	36512.0
37	39978.0
38	45501.5
39	51025.0
40	51025.0
41	53641.5
42	56258.0
43	62744.5
44	69231.0
45	69231.0
46	72245.5
47	75260.0
48	75260.0
49	79125.0
50	82990.0
51	83245.5
52	83501.0
53	83501.0
54	79611.0
55	75721.0
56	75721.0
57	73362.0
58	71003.0
59	63730.0
60	56457.0
61	56457.0
62	52964.0
63	49471.0
64	41040.5
65	32610.0
66	32610.0
67	27041.5
68	21473.0
69	21473.0
70	17213.0
71	12953.0
72	10158.0
73	7363.0
74	7363.0
75	5554.0
76	3745.0
77	3745.0
78	3625.0
79	3505.0
80	2721.5
81	1938.0
82	1938.0
83	2003.5
84	2069.0
85	2069.0
86	1372.5
87	676.0
88	585.5
89	495.0
90	495.0
91	366.0
92	237.0
93	205.5
94	174.0
95	174.0
96	155.5
97	137.0
98	137.0
99	283.5
100	430.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1200031498054108
2	0.05567613789493181
3	0.010869046840046448
4	0.0031054419542989853
5	1.1090864122496377E-4
6	3.327259236748913E-4
7	0.0
8	3.327259236748913E-4
9	2.2181728244992754E-4
10	3.327259236748913E-4
11	0.0017745382595994203
12	0.002329081465724239
13	0.012754493740870832
14	0.008650874015547174
15	0.024067175145817136
16	0.010979955481271411
17	0.021627185038867933
18	0.004990888855123369
19	0.00798542216819739
20	0.004879980213898405
21	0.0057672493436981154
22	0.0059890666261480435
23	0.008761782656772138
24	0.013752671511895506
25	0.015859935695169816
26	0.01763447395476924
27	0.006432701191047899
28	0.0057672493436981154
29	0.01031450363392163
30	0.0031054419542989853
31	0.0059890666261480435
32	0.006654518473497826
33	0.007652696244522499
34	0.010203594992696666
35	0.015194483847820036
36	0.013752671511895506
37	0.007874513526972427
38	0.005212706137573297
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	901643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.00257681298383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66042723692925	50.42499061914839
2	11.246787058981177	13.721659829295435
3	2.9015042301756115	5.309977040234558
4	1.1150298995475543	2.7207878838369335
5	0.56041648727599	1.7093424906158081
6	0.31854436575706874	1.165921628426328
7	0.2162970343812254	0.9236273517982917
8	0.14422821041332054	0.7038633987470225
9	0.10294494669867618	0.5651916316645068
>10	0.5734129122942286	6.6718597968452285
>50	0.0748233329267318	3.2042813079355628
>100	0.07846746782652403	9.38430442289936
>500	0.00638688686526488	2.712065190943852
>1k	7.299299274588434E-4	0.7821274076087306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2971	0.32950957307936735	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1962	0.2176027540833789	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1080	0.11978133252296087	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1013	0.1123504535608883	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	996	0.11046500666006391	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	961	0.10658320421719017	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	913	0.10125958943839192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.1090864122496375E-4	0.0
7	0.0	0.0	0.0	1.1090864122496375E-4	0.0
8	0.0	0.0	0.0	1.1090864122496375E-4	0.0
9	0.0	0.0	0.0	1.1090864122496375E-4	0.0
10	0.0	0.0	0.0	2.218172824499275E-4	0.0
11	0.0	0.0	0.0	3.3272592367489126E-4	1.1090864122496375E-4
12	0.0	0.0	0.0	3.3272592367489126E-4	1.1090864122496375E-4
13	0.0	0.0	0.0	3.3272592367489126E-4	2.218172824499275E-4
14	0.0	0.0	0.0	3.3272592367489126E-4	2.218172824499275E-4
15	0.0	0.0	0.0	3.3272592367489126E-4	3.3272592367489126E-4
16	0.0	0.0	0.0	4.43634564899855E-4	3.3272592367489126E-4
17	0.0	0.0	0.0	6.654518473497825E-4	3.3272592367489126E-4
18	0.0	0.0	0.0	6.654518473497825E-4	4.43634564899855E-4
19	0.0	0.0	0.0	8.8726912979971E-4	4.43634564899855E-4
20	0.0	0.0	0.0	9.981777710246738E-4	6.654518473497825E-4
21	0.0	0.0	0.0	0.0011090864122496377	7.763604885747463E-4
22	0.0	0.0	0.0	0.0012199950534746015	9.981777710246738E-4
23	0.0	0.0	0.0	0.0021072641832743113	9.981777710246738E-4
24	0.0	0.0	0.0	0.0038818024428737318	9.981777710246738E-4
25	0.0	0.0	0.0	0.004658162931448478	9.981777710246738E-4
26	0.0	0.0	0.0	0.006099975267373007	0.0011090864122496377
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCCCG	255	0.0	18.195417	10
CGTCGTA	160	0.0	17.999346	10
CGAGCCG	255	0.0	17.56799	15
ATCGTTT	220	0.0	17.454876	29
CATCGTT	230	0.0	17.390673	28
ATAAGTG	65	2.1021356E-4	17.228231	5
AAGACGG	225	0.0	17.064152	5
TTCCGTC	170	0.0	16.940561	16
ATACCGT	170	0.0	16.93868	6
TACCGTC	190	0.0	16.841492	7
GCATCGT	250	0.0	16.639395	27
GTCCTAG	135	4.3655746E-10	16.608568	1
CCGTCAA	155	1.0913936E-11	16.51461	18
ACCGTCG	175	0.0	16.456543	8
GTTCAAA	345	0.0	16.247513	1
TCCGTCA	160	1.8189894E-11	15.999418	17
CCGTCGT	180	0.0	15.999416	9
CGGCATC	255	0.0	15.685704	25
CAAGACG	235	0.0	15.657268	4
GGCGGGT	145	1.2187229E-9	15.44857	32
>>END_MODULE