FastQCFastQC Report
Thu 2 Feb 2017
SRR4062898_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062898_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901643
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33340.3697694098440292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24610.27294616605463584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14070.156048458203524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.12033587572908569No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10800.11978133252296087No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10550.11700861649233676No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9940.11024318937761397No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT9690.10747047334698989No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9540.10580684372861543No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9150.10148140672084184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA502.7958451E-522.4009329
CGTACTT801.2649252E-719.99972318
GTGCGTA801.2649252E-719.99972315
TGCGTAC801.2649252E-719.99972316
GTATTAT751.5072292E-619.20081
CAGTTCG507.194963E-419.19973426
TACCGGC1105.18412E-1018.9098787
CCGTGCG802.7951955E-617.99975213
GCGTACT802.7951955E-617.99975217
CGTGCGT1006.810296E-817.59975614
TAGCGGC2100.017.52356530
ATAGACG550.001364463517.4543043
GTCCTAA2500.017.2807181
CTAAACG652.0999933E-417.2305312
TCTAGCG2050.017.17049428
TATACTT753.2312884E-517.0673775
ATAACGA753.2328266E-517.06643112
CTAGCGG2200.016.72704129
GATAACG805.616705E-516.00066611
GTATAGG600.002440679616.0006661
Produced by FastQC (version 0.11.5)