##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062898_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901643
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195209190333646	32.0	32.0	32.0	32.0	32.0
2	31.311391537448856	32.0	32.0	32.0	32.0	32.0
3	31.38380600747746	32.0	32.0	32.0	32.0	32.0
4	31.47679181228047	32.0	32.0	32.0	32.0	32.0
5	31.407052458678212	32.0	32.0	32.0	32.0	32.0
6	34.92971830314215	36.0	36.0	36.0	36.0	36.0
7	34.96454028922756	36.0	36.0	36.0	36.0	36.0
8	34.9020443789837	36.0	36.0	36.0	36.0	36.0
9	34.99713966614281	36.0	36.0	36.0	36.0	36.0
10	34.85402759185176	36.0	36.0	36.0	32.0	36.0
11	35.01754796521461	36.0	36.0	36.0	36.0	36.0
12	34.92463203285558	36.0	36.0	36.0	32.0	36.0
13	34.96478096097901	36.0	36.0	36.0	36.0	36.0
14	34.921437863988295	36.0	36.0	36.0	32.0	36.0
15	34.88803107216493	36.0	36.0	36.0	32.0	36.0
16	34.89186518389207	36.0	36.0	36.0	32.0	36.0
17	34.863268499838625	36.0	36.0	36.0	32.0	36.0
18	34.8613198350123	36.0	36.0	36.0	32.0	36.0
19	34.855897511542814	36.0	36.0	36.0	32.0	36.0
20	34.83256122434267	36.0	36.0	36.0	32.0	36.0
21	34.82861509488789	36.0	36.0	36.0	32.0	36.0
22	34.81273075929165	36.0	36.0	36.0	32.0	36.0
23	34.756016516514855	36.0	36.0	36.0	32.0	36.0
24	34.73344328076634	36.0	36.0	36.0	32.0	36.0
25	34.71590529732943	36.0	36.0	36.0	32.0	36.0
26	34.650590089425634	36.0	36.0	36.0	32.0	36.0
27	34.631679057010366	36.0	36.0	36.0	32.0	36.0
28	34.601190271537625	36.0	36.0	36.0	32.0	36.0
29	34.56153599595405	36.0	36.0	36.0	32.0	36.0
30	34.53915574124127	36.0	36.0	36.0	32.0	36.0
31	34.52462338198156	36.0	36.0	36.0	32.0	36.0
32	34.49556420889421	36.0	36.0	36.0	32.0	36.0
33	34.458315541738806	36.0	36.0	36.0	32.0	36.0
34	34.44504975916188	36.0	36.0	36.0	32.0	36.0
35	34.40830572632405	36.0	36.0	36.0	32.0	36.0
36	34.39219070075407	36.0	36.0	36.0	32.0	36.0
37	34.371650420399206	36.0	36.0	36.0	32.0	36.0
38	33.942231016045156	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	19.0
22	45.0
23	218.0
24	601.0
25	1454.0
26	3034.0
27	5948.0
28	10696.0
29	16993.0
30	25065.0
31	36474.0
32	52570.0
33	83115.0
34	202198.0
35	463209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04515072796204	18.34147109781068	11.932121948892917	26.681256225334366
2	15.617489405452048	20.935226026265386	36.87279777029268	26.574486797989895
3	18.175819032588286	24.569147656001324	28.874399291071967	28.380634020338423
4	12.122647211812215	16.27207220596178	35.8119566169759	35.79332396525011
5	14.026837673003618	37.00832812986958	33.37806648529407	15.586767711832733
6	34.031318382109106	35.8941399201236	16.55755104847484	13.51699064929246
7	29.734960444257624	30.821759041035268	20.86666614909071	18.576614365616393
8	28.020110222474603	33.16922332972136	19.383539385846316	19.42712706195772
9	27.60890180398507	14.041026380439545	18.37480521064978	39.97526660492561
10	15.878189906536253	26.81944871139366	31.55452384728598	25.747837534784107
11	37.43525471101696	21.033262718913942	22.303656791739222	19.227825778329876
12	24.948288731324332	23.914728811134363	28.590314770512876	22.546667687028428
13	29.8383393852549	19.431880946096122	25.074586143946263	25.655193524702707
14	23.616442427878884	19.87715758897923	25.11969815104204	31.386701832099845
15	25.202657814678314	27.31846196332695	22.503585121827598	24.975295100167138
16	25.42125874653272	25.684999495365684	24.15623478472078	24.737506973380817
17	23.83648517206921	25.846815202912904	25.485585758443197	24.831113866574686
18	24.766565037381756	24.528111458748086	26.81637854450154	23.88894495936862
19	25.557121832033296	24.897215416744764	25.611356157592308	23.93430659362963
20	25.78215546507875	24.153794794613834	25.482036681923997	24.582013058383417
21	26.789598077283532	24.166048349620304	24.640849295894927	24.403504277201236
22	25.700637613778404	24.51214061441169	25.137443533638038	24.64977823817187
23	24.191947367195223	24.27756883822089	25.879976886639167	25.65050690794472
24	24.756028716465387	25.049049346581743	25.539376449437306	24.655545487515568
25	24.817943263512568	24.321737452336958	25.83697298927956	25.023346294870912
26	24.178443328948738	25.36516710623507	26.14951388688637	24.30687567792982
27	25.066184878460497	24.884737938935785	25.240977284750805	24.808099897852916
28	24.186734661057645	24.835772029506135	26.0867105938825	24.890782715553716
29	24.258381643288974	24.85806466639235	25.906262234609486	24.97729145570919
30	24.241967164387678	25.017662201115076	26.202721032603815	24.53764960189343
31	24.77732317558058	24.80438488403947	25.143099874340507	25.27519206603944
32	24.3318031637799	25.03906756887149	25.183914254311297	25.44521501303731
33	24.058635180442813	24.558167700520052	26.094474198768246	25.288722920268885
34	24.818803007398714	24.780428617534877	26.009739996872376	24.39102837819403
35	25.44765500314426	24.776657723733226	25.837055242485107	23.938632030637404
36	24.31106324787083	25.292826539994213	25.46351493883943	24.932595273295526
37	25.136667173149462	25.16761068405123	25.160179805089157	24.535542337710158
38	24.328197142765248	25.166779239187214	25.682949200402376	24.822074417645158
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	262.0
1	208.5
2	155.0
3	155.0
4	471.5
5	788.0
6	788.0
7	898.0
8	1008.0
9	1038.5
10	1069.0
11	1069.0
12	1292.5
13	1516.0
14	1971.0
15	2426.0
16	2426.0
17	3663.0
18	4900.0
19	4900.0
20	5598.0
21	6296.0
22	6449.0
23	6602.0
24	6602.0
25	7776.5
26	8951.0
27	8951.0
28	11676.0
29	14401.0
30	18186.5
31	21972.0
32	21972.0
33	27581.5
34	33191.0
35	33191.0
36	36667.0
37	40143.0
38	45631.5
39	51120.0
40	51120.0
41	53544.0
42	55968.0
43	62529.5
44	69091.0
45	69091.0
46	72447.5
47	75804.0
48	75804.0
49	79679.0
50	83554.0
51	83380.0
52	83206.0
53	83206.0
54	79555.0
55	75904.0
56	75904.0
57	73696.0
58	71488.0
59	63903.5
60	56319.0
61	56319.0
62	53056.0
63	49793.0
64	41070.0
65	32347.0
66	32347.0
67	26926.5
68	21506.0
69	21506.0
70	17264.0
71	13022.0
72	10200.0
73	7378.0
74	7378.0
75	5560.5
76	3743.0
77	3743.0
78	3617.0
79	3491.0
80	2619.5
81	1748.0
82	1748.0
83	1708.0
84	1668.0
85	1668.0
86	1043.0
87	418.0
88	365.5
89	313.0
90	313.0
91	180.5
92	48.0
93	32.0
94	16.0
95	16.0
96	12.5
97	9.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00942723450412192
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.4363456489985507E-4
8	0.0013309036946995653
9	0.0031054419542989853
10	0.0011090864122496377
11	0.003659985160423804
12	2.2181728244992754E-4
13	1.1090864122496377E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.2181728244992754E-4
22	0.0
23	0.0
24	0.0
25	1.1090864122496377E-4
26	1.1090864122496377E-4
27	2.2181728244992754E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.2181728244992754E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	901643.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.16720784166461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4739428262303	48.20585708879469
2	11.991118470084858	14.189619975470686
3	3.1289876138674724	5.55400381451073
4	1.1791671103678258	2.7907210199675294
5	0.6010971777599267	1.7782620824779802
6	0.34833783433752386	1.2366106226018165
7	0.21901650910679718	0.907101672055439
8	0.1566589059311171	0.7415256037979353
9	0.11876603912012974	0.6324349429036789
>10	0.6109860039494727	6.856062556971435
>50	0.08003154331062978	3.3456051951234738
>100	0.0849228988803967	10.156145052330553
>500	0.00583727239631696	2.444921538460635
>1k	0.0011297946573516698	1.161128834533458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3334	0.3697694098440292	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2461	0.27294616605463584	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1407	0.156048458203524	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1085	0.12033587572908569	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1080	0.11978133252296087	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1055	0.11700861649233676	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	994	0.11024318937761397	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	969	0.10747047334698989	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	954	0.10580684372861543	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	915	0.10148140672084184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1090864122496375E-4	0.0
2	0.0	0.0	0.0	1.1090864122496375E-4	0.0
3	0.0	0.0	0.0	1.1090864122496375E-4	0.0
4	0.0	0.0	0.0	1.1090864122496375E-4	0.0
5	0.0	0.0	0.0	1.1090864122496375E-4	0.0
6	0.0	0.0	0.0	1.1090864122496375E-4	0.0
7	0.0	0.0	0.0	1.1090864122496375E-4	0.0
8	0.0	0.0	0.0	1.1090864122496375E-4	0.0
9	0.0	0.0	0.0	1.1090864122496375E-4	0.0
10	0.0	0.0	0.0	2.218172824499275E-4	0.0
11	0.0	0.0	0.0	2.218172824499275E-4	0.0
12	0.0	0.0	0.0	2.218172824499275E-4	4.43634564899855E-4
13	0.0	0.0	0.0	2.218172824499275E-4	5.545432061248188E-4
14	0.0	0.0	0.0	2.218172824499275E-4	5.545432061248188E-4
15	0.0	0.0	0.0	2.218172824499275E-4	7.763604885747463E-4
16	0.0	0.0	0.0	3.3272592367489126E-4	7.763604885747463E-4
17	0.0	0.0	0.0	3.3272592367489126E-4	8.8726912979971E-4
18	0.0	0.0	0.0	3.3272592367489126E-4	9.981777710246738E-4
19	0.0	0.0	0.0	5.545432061248188E-4	9.981777710246738E-4
20	0.0	0.0	0.0	5.545432061248188E-4	9.981777710246738E-4
21	0.0	0.0	0.0	7.763604885747463E-4	9.981777710246738E-4
22	0.0	0.0	0.0	9.981777710246738E-4	9.981777710246738E-4
23	0.0	0.0	0.0	0.00177453825959942	9.981777710246738E-4
24	0.0	0.0	0.0	0.00354907651919884	9.981777710246738E-4
25	0.0	0.0	0.0	0.004103619725323659	9.981777710246738E-4
26	0.0	0.0	0.0	0.005656340702473152	0.0011090864122496377
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTAA	50	2.7958451E-5	22.400932	9
CGTACTT	80	1.2649252E-7	19.999723	18
GTGCGTA	80	1.2649252E-7	19.999723	15
TGCGTAC	80	1.2649252E-7	19.999723	16
GTATTAT	75	1.5072292E-6	19.2008	1
CAGTTCG	50	7.194963E-4	19.199734	26
TACCGGC	110	5.18412E-10	18.909878	7
CCGTGCG	80	2.7951955E-6	17.999752	13
GCGTACT	80	2.7951955E-6	17.999752	17
CGTGCGT	100	6.810296E-8	17.599756	14
TAGCGGC	210	0.0	17.523565	30
ATAGACG	55	0.0013644635	17.454304	3
GTCCTAA	250	0.0	17.280718	1
CTAAACG	65	2.0999933E-4	17.23053	12
TCTAGCG	205	0.0	17.170494	28
TATACTT	75	3.2312884E-5	17.067377	5
ATAACGA	75	3.2328266E-5	17.066431	12
CTAGCGG	220	0.0	16.727041	29
GATAACG	80	5.616705E-5	16.000666	11
GTATAGG	60	0.0024406796	16.000666	1
>>END_MODULE