FastQCFastQC Report
Thu 2 Feb 2017
SRR4062897_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062897_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531233
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35250.23020663739613764No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT30450.19885935060176993No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC30310.19794505473693425No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25780.16836105282474972No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT25670.1676426775023788No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24380.1592180941763925No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24220.15817318461658025No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24000.15673643397183837No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22720.14837715749334032No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22290.1455689630513449No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21940.1432832233892556No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21690.14165055220204892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21530.14060564264223668No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20980.13701376603038204No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20410.1332912757235509No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19880.12983001280667278No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19880.12983001280667278No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19570.12780550053453654No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18900.12342994175282272No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18740.12238503219301046No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18370.11996867883594463No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT17530.11448290364693028No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16550.10808283259308021No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT16090.10507871760861998No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16030.10468687652369038No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15890.10377258065885465No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC15880.1037072738113664No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15830.10338073957392507No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG15370.10037662458946484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT350.002068716822.8535966
AATATCG400.004488175319.9968995
CTAGCGG6100.019.67229329
TCTAGCG6200.019.35373328
AATACCG507.2049466E-419.1970235
TAGCGGC6500.018.9546230
CGGTCCA7650.017.14934710
CGCCGGT7750.017.1322657
ATCGTTT4600.016.3479629
CCGGTCC8700.016.1829839
AGCGGCG7550.016.10661731
CGCACGA600.002443335315.99908428
GGACCGT1001.3171957E-615.99751956
GCCGGTC8500.015.9969968
ATAACGA3150.015.745645512
GTCCTAT5350.015.5688181
ACGAACG3300.015.51527715
CAAGACG7050.015.4307344
TAACGAA3250.015.26166313
CCGATAA3250.015.2606669
Produced by FastQC (version 0.11.5)