##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062897_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1531233
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264369302385724	32.0	32.0	32.0	32.0	32.0
2	30.863758813975405	32.0	32.0	32.0	32.0	32.0
3	30.901629601765375	32.0	32.0	32.0	32.0	32.0
4	30.979498221368008	32.0	32.0	32.0	32.0	32.0
5	30.872690831506375	32.0	32.0	32.0	32.0	32.0
6	34.550577214571526	36.0	36.0	36.0	32.0	36.0
7	34.47902964473728	36.0	36.0	36.0	32.0	36.0
8	34.44938164211456	36.0	36.0	36.0	32.0	36.0
9	34.61130866432476	36.0	36.0	36.0	32.0	36.0
10	34.30203176133221	36.0	36.0	36.0	32.0	36.0
11	34.58338868088658	36.0	36.0	36.0	32.0	36.0
12	34.381963424247	36.0	36.0	36.0	32.0	36.0
13	34.487078060621734	36.0	36.0	36.0	32.0	36.0
14	34.37596825564757	36.0	36.0	36.0	32.0	36.0
15	34.303672269341114	36.0	36.0	36.0	32.0	36.0
16	34.324646216480446	36.0	36.0	36.0	32.0	36.0
17	34.23316699679278	36.0	36.0	36.0	32.0	36.0
18	34.2664630399162	36.0	36.0	36.0	32.0	36.0
19	34.252957583855626	36.0	36.0	36.0	32.0	36.0
20	34.2425111005314	36.0	36.0	36.0	32.0	36.0
21	34.22629671643701	36.0	36.0	36.0	32.0	36.0
22	34.20283849681923	36.0	36.0	36.0	32.0	36.0
23	34.1303701004354	36.0	36.0	36.0	32.0	36.0
24	34.10568672435873	36.0	36.0	36.0	32.0	36.0
25	34.07690403746523	36.0	36.0	36.0	32.0	36.0
26	34.019332132993476	36.0	36.0	36.0	32.0	36.0
27	34.024586721942384	36.0	36.0	36.0	32.0	36.0
28	34.00467923562253	36.0	36.0	36.0	32.0	36.0
29	33.96261770742924	36.0	36.0	36.0	32.0	36.0
30	33.93297231708042	36.0	36.0	36.0	32.0	36.0
31	33.962362357655564	36.0	36.0	36.0	32.0	36.0
32	33.91758341153828	36.0	36.0	36.0	32.0	36.0
33	33.87178894394256	36.0	36.0	36.0	32.0	36.0
34	33.88320066247266	36.0	36.0	36.0	32.0	36.0
35	33.831732989035636	36.0	36.0	36.0	32.0	36.0
36	33.78267970975025	36.0	36.0	36.0	32.0	36.0
37	33.78323089954305	36.0	36.0	36.0	32.0	36.0
38	33.1509600433115	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	2.0
8	6.0
9	2.0
10	11.0
11	18.0
12	6.0
13	7.0
14	119.0
15	332.0
16	407.0
17	534.0
18	695.0
19	921.0
20	1361.0
21	1828.0
22	2924.0
23	4633.0
24	6835.0
25	10088.0
26	14976.0
27	21398.0
28	30184.0
29	41435.0
30	55204.0
31	75367.0
32	102633.0
33	152859.0
34	337875.0
35	668570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.59151614221496	16.51982018798529	10.982394769105026	25.90626890069473
2	17.55311780403719	18.961076130509444	35.973998478801235	27.511807586652132
3	19.669154506687253	22.805700935372982	27.355528602322465	30.169615955617303
4	12.931984837916701	14.383015018404011	34.63957397785249	38.0454261658268
5	15.566223798875676	35.59121021504253	32.52145984409939	16.3211061419824
6	36.116870827289716	33.96807401113746	16.123910923839606	13.791144237733219
7	31.84725230876334	29.258877334641216	20.006387018026672	18.88748333856877
8	29.667534380947032	31.359969070535637	18.776588171830937	20.195908376686393
9	27.766668321112203	13.993343904369565	17.97204596572163	40.2679418087966
10	16.828878838838186	25.25670623193848	29.79973550588581	28.114679423337524
11	39.14732403214988	20.60327729242955	20.829765876895486	19.41963279852508
12	25.703229851389608	22.998984446686716	27.09907685877278	24.198708843150893
13	29.911529279595563	18.123859008566214	25.04508447010643	26.919527241731796
14	24.6030616017044	19.017317029522683	23.45238110788756	32.927240260885355
15	26.10214553305179	25.932371125939426	21.65443493995186	26.311048401056926
16	27.36645888088284	24.747757115313274	22.425777106639565	25.460006897164323
17	25.146350863212074	25.02910033901405	24.031262451744386	25.793286346029486
18	26.683355778512453	23.270534726458358	25.110538697361047	24.935570797668138
19	26.30108392092524	24.390702680673083	24.28770537414735	25.02050802425433
20	26.55138092103201	22.92953279622141	24.038501729416804	26.480584553329777
21	28.10200028345023	23.341865141695724	23.171599070753487	25.38453550410056
22	26.915176749122377	23.128288023512123	24.04676300106131	25.909772226304188
23	25.001012355967873	22.86611690294837	24.876002722257855	27.2568680188259
24	25.526596477752285	24.344175188813143	24.12262224112041	26.006606092314165
25	25.847532355219617	23.474794477241286	24.037312194720485	26.640360972818613
26	25.48354500164279	24.675083756114734	24.654508067955945	25.18686317428653
27	26.64016281126355	23.613446036765772	23.848636729980708	25.897754421989973
28	25.40433819418683	23.411841080917277	24.99457921658577	26.189241508310126
29	25.046993279209968	24.026334524221625	25.064693319051383	25.86197887751703
30	25.1774927850187	24.171085346093786	25.255732636532784	25.395689232354723
31	25.831798529200466	24.33206630353854	23.515028018339276	26.32110714892172
32	25.73168278550193	23.785731826470418	23.427368580549764	27.055216807477883
33	24.890029684637465	23.551641145715973	24.453871249008063	27.1044579206385
34	26.352218100114953	23.8515649493155	24.474670811997072	25.321546138572476
35	27.071109401239333	23.304369295725152	24.690018609007698	24.934502694027806
36	25.227320082221254	24.63156217116887	23.905365870864035	26.235751875745837
37	26.840312588785075	24.149541011766164	23.935052593422313	25.075093806026445
38	25.346215339279276	23.701088347936945	24.474962250053554	26.477734062730224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	108.5
2	149.0
3	149.0
4	416.0
5	683.0
6	683.0
7	834.0
8	985.0
9	977.5
10	970.0
11	970.0
12	1267.0
13	1564.0
14	2020.5
15	2477.0
16	2477.0
17	3624.5
18	4772.0
19	4772.0
20	5774.5
21	6777.0
22	6851.5
23	6926.0
24	6926.0
25	7980.0
26	9034.0
27	9034.0
28	11735.5
29	14437.0
30	18251.5
31	22066.0
32	22066.0
33	30265.0
34	38464.0
35	38464.0
36	44615.5
37	50767.0
38	60166.0
39	69565.0
40	69565.0
41	75145.5
42	80726.0
43	96475.0
44	112224.0
45	112224.0
46	119385.0
47	126546.0
48	126546.0
49	135730.5
50	144915.0
51	148851.5
52	152788.0
53	152788.0
54	147191.5
55	141595.0
56	141595.0
57	139187.0
58	136779.0
59	124518.0
60	112257.0
61	112257.0
62	107615.5
63	102974.0
64	86249.5
65	69525.0
66	69525.0
67	58557.0
68	47589.0
69	47589.0
70	38093.0
71	28597.0
72	22223.0
73	15849.0
74	15849.0
75	11916.5
76	7984.0
77	7984.0
78	7712.5
79	7441.0
80	5918.0
81	4395.0
82	4395.0
83	4469.5
84	4544.0
85	4544.0
86	3056.0
87	1568.0
88	1342.0
89	1116.0
90	1116.0
91	814.5
92	513.0
93	468.5
94	424.0
95	424.0
96	385.5
97	347.0
98	347.0
99	590.0
100	833.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12134012263319821
2	0.05622919568739702
3	0.012147073632817475
4	0.0029388081369719696
5	6.5306847488266E-5
6	2.61227389953064E-4
7	1.30613694976532E-4
8	4.571479324178619E-4
9	3.2653423744132996E-4
10	5.22454779906128E-4
11	0.00195920542464798
12	0.0031347286794367675
13	0.014236892752441988
14	0.009077651800868973
15	0.02468598835056455
16	0.011036857225516953
17	0.021094111738709915
18	0.0062041505113852695
19	0.00816335593603325
20	0.005289854646549546
21	0.0065306847488266
22	0.007053139528732728
23	0.009861333970728164
24	0.014498120142395049
25	0.016914473499460892
26	0.019592054246479796
27	0.008816424410915909
28	0.005812309426455673
29	0.010645016140587356
30	0.0028735012894837036
31	0.0060735368164087375
32	0.006857218986267929
33	0.009012344953380708
34	0.012604221565235335
35	0.016587939262019562
36	0.015804257092160368
37	0.009665413428263367
38	0.006857218986267929
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1531233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.15758362149322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52756664027929	35.323275565480856
2	13.258151604065088	12.239284826621374
3	4.399796237388347	6.092518882343515
4	1.900665434901019	3.509204949917022
5	1.0277247937937675	2.371864655470884
6	0.6125309509960549	1.6963769154811899
7	0.4199231407329782	1.3567846238087755
8	0.30072255802060394	1.1104501294964297
9	0.2158942361943807	0.8968640634486481
>10	1.109475226199092	9.215376955575088
>50	0.09752098765035436	3.1750439984755303
>100	0.10291537927351307	10.468392899251883
>500	0.01831179348272332	5.9016580566241865
>1k	0.008801017022704232	6.642903478004743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3525	0.23020663739613764	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3045	0.19885935060176993	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3031	0.19794505473693425	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2578	0.16836105282474972	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2567	0.1676426775023788	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2438	0.1592180941763925	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2422	0.15817318461658025	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2400	0.15673643397183837	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2272	0.14837715749334032	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2229	0.1455689630513449	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2194	0.1432832233892556	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2169	0.14165055220204892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2153	0.14060564264223668	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2098	0.13701376603038204	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2041	0.1332912757235509	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1988	0.12983001280667278	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1988	0.12983001280667278	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1957	0.12780550053453654	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1890	0.12342994175282272	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1874	0.12238503219301046	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1837	0.11996867883594463	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1753	0.11448290364693028	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1655	0.10808283259308021	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1609	0.10507871760861998	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1603	0.10468687652369038	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1589	0.10377258065885465	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1588	0.1037072738113664	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1583	0.10338073957392507	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1537	0.10037662458946484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.30613694976532E-4
2	0.0	6.5306847488266E-5	0.0	6.5306847488266E-5	1.30613694976532E-4
3	0.0	6.5306847488266E-5	0.0	1.30613694976532E-4	1.30613694976532E-4
4	0.0	6.5306847488266E-5	0.0	1.9592054246479797E-4	1.30613694976532E-4
5	0.0	6.5306847488266E-5	0.0	1.9592054246479797E-4	1.9592054246479797E-4
6	0.0	6.5306847488266E-5	0.0	1.9592054246479797E-4	3.2653423744132996E-4
7	0.0	6.5306847488266E-5	0.0	2.61227389953064E-4	3.2653423744132996E-4
8	0.0	6.5306847488266E-5	0.0	2.61227389953064E-4	3.2653423744132996E-4
9	0.0	6.5306847488266E-5	0.0	2.61227389953064E-4	3.2653423744132996E-4
10	0.0	6.5306847488266E-5	0.0	2.61227389953064E-4	3.9184108492959594E-4
11	0.0	6.5306847488266E-5	0.0	2.61227389953064E-4	3.9184108492959594E-4
12	0.0	6.5306847488266E-5	0.0	4.571479324178619E-4	8.489890173474579E-4
13	0.0	6.5306847488266E-5	0.0	4.571479324178619E-4	9.7960271232399E-4
14	0.0	6.5306847488266E-5	0.0	4.571479324178619E-4	9.7960271232399E-4
15	0.0	6.5306847488266E-5	0.0	4.571479324178619E-4	0.0011755232547887879
16	0.0	6.5306847488266E-5	0.0	5.877616273943939E-4	0.0011755232547887879
17	0.0	6.5306847488266E-5	0.0	6.530684748826599E-4	0.0011755232547887879
18	0.0	6.5306847488266E-5	0.0	7.836821698591919E-4	0.0012408301022770539
19	0.0	6.5306847488266E-5	0.0	7.836821698591919E-4	0.0012408301022770539
20	0.0	6.5306847488266E-5	0.0	9.142958648357238E-4	0.0013061369497653198
21	0.0	6.5306847488266E-5	0.0	0.001044909559812256	0.0014367506447418518
22	0.0	6.5306847488266E-5	0.0	0.001110216407300522	0.0016326711872066497
23	0.0	6.5306847488266E-5	0.0	0.0018938985771597139	0.0016326711872066497
24	0.0	6.5306847488266E-5	0.0	0.0025469670520423737	0.0016326711872066497
25	0.0	6.5306847488266E-5	0.0	0.0030694218319485015	0.0016326711872066497
26	0.0	6.5306847488266E-5	0.0	0.004179638239249024	0.0018285917296714477
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGT	35	0.0020687168	22.853596	6
AATATCG	40	0.0044881753	19.996899	5
CTAGCGG	610	0.0	19.672293	29
TCTAGCG	620	0.0	19.353733	28
AATACCG	50	7.2049466E-4	19.197023	5
TAGCGGC	650	0.0	18.95462	30
CGGTCCA	765	0.0	17.149347	10
CGCCGGT	775	0.0	17.132265	7
ATCGTTT	460	0.0	16.34796	29
CCGGTCC	870	0.0	16.182983	9
AGCGGCG	755	0.0	16.106617	31
CGCACGA	60	0.0024433353	15.999084	28
GGACCGT	100	1.3171957E-6	15.9975195	6
GCCGGTC	850	0.0	15.996996	8
ATAACGA	315	0.0	15.7456455	12
GTCCTAT	535	0.0	15.568818	1
ACGAACG	330	0.0	15.515277	15
CAAGACG	705	0.0	15.430734	4
TAACGAA	325	0.0	15.261663	13
CCGATAA	325	0.0	15.260666	9
>>END_MODULE