FastQCFastQC Report
Thu 2 Feb 2017
SRR4062897_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062897_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531233
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37200.24294147265634952No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC33770.22054122396787423No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31190.20369205731590162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25050.16359365295810632No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24840.16222220916085273No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24820.16209159546587618No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24790.1618956749234114No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24540.16026300373620475No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24380.1592180941763925No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24060.157128275056768No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT22910.1496179875956174No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22740.14850777118831687No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22550.1472669410860398No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22120.14445874664404437No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21710.14178116589702547No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21210.13851582352261216No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC19430.12689120466970083No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19090.12467077185509978No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG18120.11833600764873799No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17870.11670333646153133No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17370.11343799408711802No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG17280.11285023245972364No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC16810.10978081062777514No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16660.10880120791545114No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16590.10834405998303327No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16350.10677669564331489No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16220.10592770662596744No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15870.10364196696387813No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT15820.10331543272643681No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15730.1027276710990424No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15670.10233583001411281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15590.10181337523420668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5850.020.23824128
TAGCGAA400.004482137520.0015510
GTAGTAT400.004482137520.001551
ATCGTTT4500.019.91005529
CTAGCGG6050.019.83365829
CATCGTT4650.019.26779428
CCTATAC507.1952573E-419.2008633
TAGCGGC6550.018.3196430
TCGTTTA4850.018.14336630
ATTACGA550.001365411817.45361925
CAAGACG7100.017.128094
CGTTTAT5200.016.92217831
GTATTAC957.690269E-716.843411
AGCGGCG7450.016.7507931
GCGCAAG6900.016.6969471
CGTCTTA1151.8433639E-816.69476715
AAGACGG7100.016.6789865
GACGGAC6950.016.578457
TCTACAC2800.016.5721743
CGCAAGA7150.016.5596452
Produced by FastQC (version 0.11.5)