##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062897_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1531233
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199434050859665	32.0	32.0	32.0	32.0	32.0
2	31.28929235459267	32.0	32.0	32.0	32.0	32.0
3	31.37567764017625	32.0	32.0	32.0	32.0	32.0
4	31.4797467139227	32.0	32.0	32.0	32.0	32.0
5	31.386181593526263	32.0	32.0	32.0	32.0	32.0
6	34.94165029097466	36.0	36.0	36.0	36.0	36.0
7	34.96559765888013	36.0	36.0	36.0	36.0	36.0
8	34.898613731548366	36.0	36.0	36.0	36.0	36.0
9	35.01590744191119	36.0	36.0	36.0	36.0	36.0
10	34.853608823738774	36.0	36.0	36.0	32.0	36.0
11	35.03256264722612	36.0	36.0	36.0	36.0	36.0
12	34.921352922775306	36.0	36.0	36.0	32.0	36.0
13	34.97826914649828	36.0	36.0	36.0	36.0	36.0
14	34.915082159279486	36.0	36.0	36.0	32.0	36.0
15	34.88453814670922	36.0	36.0	36.0	32.0	36.0
16	34.88774993746869	36.0	36.0	36.0	32.0	36.0
17	34.849811230557336	36.0	36.0	36.0	32.0	36.0
18	34.85651954993133	36.0	36.0	36.0	32.0	36.0
19	34.850277521448405	36.0	36.0	36.0	32.0	36.0
20	34.83656504268129	36.0	36.0	36.0	32.0	36.0
21	34.82531463206448	36.0	36.0	36.0	32.0	36.0
22	34.79948381467745	36.0	36.0	36.0	32.0	36.0
23	34.74593024053165	36.0	36.0	36.0	32.0	36.0
24	34.71678379449764	36.0	36.0	36.0	32.0	36.0
25	34.6848533175552	36.0	36.0	36.0	32.0	36.0
26	34.61824294539107	36.0	36.0	36.0	32.0	36.0
27	34.59911130441938	36.0	36.0	36.0	32.0	36.0
28	34.567876345402695	36.0	36.0	36.0	32.0	36.0
29	34.52739132450777	36.0	36.0	36.0	32.0	36.0
30	34.50081666212784	36.0	36.0	36.0	32.0	36.0
31	34.50730424435733	36.0	36.0	36.0	32.0	36.0
32	34.47301880249446	36.0	36.0	36.0	32.0	36.0
33	34.43219941054039	36.0	36.0	36.0	32.0	36.0
34	34.42964134132428	36.0	36.0	36.0	32.0	36.0
35	34.38263020715985	36.0	36.0	36.0	32.0	36.0
36	34.35763923583151	36.0	36.0	36.0	32.0	36.0
37	34.35065140315027	36.0	36.0	36.0	32.0	36.0
38	33.894935649897825	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	24.0
22	101.0
23	363.0
24	939.0
25	2502.0
26	5281.0
27	10222.0
28	18168.0
29	28398.0
30	43084.0
31	62204.0
32	89709.0
33	143950.0
34	353161.0
35	773120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89587267085411	17.783378736805943	11.677847176548426	26.642901415791524
2	16.46790527633613	19.895665780452745	35.33093918430442	28.30548975890671
3	18.664109250519026	23.423215147531433	27.732095637959738	30.180579963989807
4	12.337010116050495	15.160426585163561	34.6972042083815	37.805359090404444
5	14.914003887066102	36.11954295626006	32.32455957033291	16.641893586340935
6	36.10313277561176	34.409200446699714	15.704825532415118	13.782841245273408
7	31.551401429707777	29.448505833902594	20.018586462315717	18.981506274073908
8	29.4874114168793	31.568800455071187	18.716043545244904	20.227744582804604
9	27.530770506813724	13.754021424083742	17.889279729149006	40.82592833995353
10	16.665491131896307	25.484026441695434	29.889342993616847	27.96113943279141
11	39.21235035068115	20.506765119063093	20.783221642633738	19.49766288762202
12	25.43718207685732	23.097555097970453	27.103819355327456	24.361443469844772
13	29.86324719343273	18.152661585783978	25.001795941824547	26.982295278958745
14	24.376891041402583	18.992145545452583	23.43418669790946	33.19677671523537
15	26.079505862269166	26.14102491260311	21.402490672549508	26.37697855257822
16	27.281318102885848	24.789009626894963	22.352995726967386	25.576676543251804
17	25.122695239718578	25.128507549145034	23.89309791520951	25.855699295926875
18	26.616850603402618	23.155783607066986	25.165471224823392	25.061894564707003
19	26.331067838793963	24.33633548911237	24.23870175211741	25.093894919976258
20	26.588638045287688	22.843159728140655	24.03376886469923	26.534433361872427
21	28.08008719781666	23.311766807228956	23.166132347111574	25.442013647842813
22	26.838044895845375	23.131750687191303	24.08660210431724	25.943602312646085
23	24.957158680069384	22.74403878706819	24.944358529602304	27.35444400326012
24	25.502519864710333	24.25724889680408	24.214799445936706	26.02543179254888
25	25.903341821057698	23.36316336333316	24.14743431918502	26.586060496424118
26	25.6044805751186	24.52430461223381	24.634281415226432	25.23693339742116
27	26.606780170189975	23.44481234040608	23.84024607668345	26.10816141272049
28	25.34114664456683	23.350332705734527	25.107935892186234	26.200584757512406
29	25.09983784309769	23.853652579326596	25.13614845030116	25.910361127274555
30	25.148164910239	24.0194666650993	25.342844622601525	25.48952380206017
31	25.853544169959765	24.045654710942095	23.61913568999623	26.48166542910191
32	25.80208237413901	23.643756371499308	23.631543991019	26.92261726334268
33	24.910186757991763	23.34223465664598	24.634461247896304	27.11311733746595
34	26.179686566316164	23.62403370355785	24.669008570217596	25.527271159908388
35	27.04833294475759	23.16486125886785	24.789434396985957	24.997371399388594
36	25.311105494722224	24.466361422461507	24.03500969480151	26.187523388014753
37	26.91569473750892	23.968919165143383	24.024691212898365	25.090694884449327
38	25.42673182557041	23.421090612716174	24.58708059071427	26.565096970999154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	43.0
2	67.0
3	67.0
4	322.5
5	578.0
6	578.0
7	739.5
8	901.0
9	949.0
10	997.0
11	997.0
12	1249.5
13	1502.0
14	2028.5
15	2555.0
16	2555.0
17	3778.5
18	5002.0
19	5002.0
20	5879.5
21	6757.0
22	6974.5
23	7192.0
24	7192.0
25	7947.5
26	8703.0
27	8703.0
28	11372.0
29	14041.0
30	18054.0
31	22067.0
32	22067.0
33	30510.5
34	38954.0
35	38954.0
36	44788.0
37	50622.0
38	59799.0
39	68976.0
40	68976.0
41	74985.5
42	80995.0
43	96459.0
44	111923.0
45	111923.0
46	119729.5
47	127536.0
48	127536.0
49	136841.5
50	146147.0
51	149310.5
52	152474.0
53	152474.0
54	146954.5
55	141435.0
56	141435.0
57	140081.5
58	138728.0
59	125803.0
60	112878.0
61	112878.0
62	107964.5
63	103051.0
64	86434.5
65	69818.0
66	69818.0
67	58909.5
68	48001.0
69	48001.0
70	38306.5
71	28612.0
72	22120.5
73	15629.0
74	15629.0
75	11576.0
76	7523.0
77	7523.0
78	7509.0
79	7495.0
80	5841.5
81	4188.0
82	4188.0
83	4019.0
84	3850.0
85	3850.0
86	2464.5
87	1079.0
88	907.0
89	735.0
90	735.0
91	425.5
92	116.0
93	82.5
94	49.0
95	49.0
96	33.5
97	18.0
98	18.0
99	19.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009534799733286834
2	0.0
3	0.0
4	1.9592054246479797E-4
5	6.5306847488266E-5
6	1.9592054246479797E-4
7	7.183753223709259E-4
8	0.0028735012894837036
9	0.0038531040018076933
10	9.7960271232399E-4
11	0.0045714793241786195
12	3.9184108492959594E-4
13	1.9592054246479797E-4
14	0.0
15	0.0
16	1.30613694976532E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.30613694976532E-4
22	0.0
23	6.5306847488266E-5
24	0.0
25	1.30613694976532E-4
26	6.5306847488266E-5
27	1.9592054246479797E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.30613694976532E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1531233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.94736506980259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72122974988096	32.83801162282614
2	14.32248449585844	12.58870909692157
3	4.781035780109403	6.303417745207692
4	1.9823783967236168	3.4848122842921097
5	1.066607786084962	2.3437300880684875
6	0.6572048631304951	1.7329453227387308
7	0.44743282097174863	1.3764445469219946
8	0.31611693200044755	1.1114004972295703
9	0.2319212723225852	0.9173095939992382
>10	1.2298957872559522	9.728598210243396
>50	0.10308141137092258	3.2124282218085907
>100	0.11042651123908798	10.842370499440749
>500	0.01912661737911208	5.754188920685614
>1k	0.011057575672299173	7.765633349616048
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3720	0.24294147265634952	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3377	0.22054122396787423	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3119	0.20369205731590162	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2505	0.16359365295810632	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2484	0.16222220916085273	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2482	0.16209159546587618	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2479	0.1618956749234114	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2454	0.16026300373620475	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2438	0.1592180941763925	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2406	0.157128275056768	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2291	0.1496179875956174	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2274	0.14850777118831687	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2255	0.1472669410860398	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2212	0.14445874664404437	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2171	0.14178116589702547	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2121	0.13851582352261216	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1943	0.12689120466970083	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1909	0.12467077185509978	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1812	0.11833600764873799	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1787	0.11670333646153133	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1737	0.11343799408711802	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1728	0.11285023245972364	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1681	0.10978081062777514	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1666	0.10880120791545114	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1659	0.10834405998303327	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1635	0.10677669564331489	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1622	0.10592770662596744	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1587	0.10364196696387813	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1582	0.10331543272643681	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1573	0.1027276710990424	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1567	0.10233583001411281	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1559	0.10181337523420668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9592054246479797E-4
2	0.0	0.0	0.0	0.0	1.9592054246479797E-4
3	0.0	0.0	0.0	0.0	1.9592054246479797E-4
4	0.0	0.0	0.0	0.0	1.9592054246479797E-4
5	0.0	0.0	0.0	0.0	1.9592054246479797E-4
6	0.0	0.0	0.0	0.0	3.2653423744132996E-4
7	0.0	0.0	0.0	0.0	3.2653423744132996E-4
8	0.0	0.0	0.0	0.0	3.2653423744132996E-4
9	0.0	0.0	0.0	6.5306847488266E-5	3.2653423744132996E-4
10	0.0	0.0	0.0	6.5306847488266E-5	3.2653423744132996E-4
11	0.0	0.0	0.0	6.5306847488266E-5	3.2653423744132996E-4
12	0.0	0.0	0.0	1.9592054246479797E-4	0.001044909559812256
13	0.0	0.0	0.0	2.61227389953064E-4	0.0011755232547887879
14	0.0	0.0	0.0	2.61227389953064E-4	0.0011755232547887879
15	0.0	0.0	0.0	2.61227389953064E-4	0.0014367506447418518
16	0.0	0.0	0.0	4.571479324178619E-4	0.0015673643397183838
17	0.0	0.0	0.0	5.22454779906128E-4	0.0015673643397183838
18	0.0	0.0	0.0	6.530684748826599E-4	0.0016326711872066497
19	0.0	0.0	0.0	6.530684748826599E-4	0.0016326711872066497
20	0.0	0.0	0.0	7.183753223709259E-4	0.002024512272136246
21	0.0	0.0	0.0	7.836821698591919E-4	0.002089819119624512
22	0.0	0.0	0.0	9.142958648357238E-4	0.002220432814601044
23	0.0	0.0	0.0	0.0016979780346949157	0.002220432814601044
24	0.0	0.0	0.0	0.002024512272136246	0.002220432814601044
25	0.0	0.0	0.0	0.0025469670520423737	0.002220432814601044
26	0.0	0.0	0.0	0.0034612629168780974	0.0022857396620893098
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	585	0.0	20.238241	28
TAGCGAA	40	0.0044821375	20.00155	10
GTAGTAT	40	0.0044821375	20.00155	1
ATCGTTT	450	0.0	19.910055	29
CTAGCGG	605	0.0	19.833658	29
CATCGTT	465	0.0	19.267794	28
CCTATAC	50	7.1952573E-4	19.200863	3
TAGCGGC	655	0.0	18.31964	30
TCGTTTA	485	0.0	18.143366	30
ATTACGA	55	0.0013654118	17.453619	25
CAAGACG	710	0.0	17.12809	4
CGTTTAT	520	0.0	16.922178	31
GTATTAC	95	7.690269E-7	16.84341	1
AGCGGCG	745	0.0	16.75079	31
GCGCAAG	690	0.0	16.696947	1
CGTCTTA	115	1.8433639E-8	16.694767	15
AAGACGG	710	0.0	16.678986	5
GACGGAC	695	0.0	16.57845	7
TCTACAC	280	0.0	16.572174	3
CGCAAGA	715	0.0	16.559645	2
>>END_MODULE