FastQCFastQC Report
Thu 2 Feb 2017
SRR4062896_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062896_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970470
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA186280.9453582140301552No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC151890.7708313245063361No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA137780.6992240429948184No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG121190.6150309317066487No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA73320.3720939674290905No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA70250.35651392814912175No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA65690.33337224114043856No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA50080.254152562586591No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA48430.245778925840028No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA42480.21558308423878567No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT39230.19908955731373734No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA38930.19756707790527134No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG35670.18102280166660747No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG33490.1699594512984212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33400.1695027074758814No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT33140.16818322532187752No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT32240.1636157870964795No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG31420.15945434338000578No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT30620.15539439829076312No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT29650.1504717148700564No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA29310.14874623820712826No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA29290.14864473957989718No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA28660.14544753282211859No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC27000.13702314676194005No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA26240.1331661989271595No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA24620.12494481012144312No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT24540.12453881561251884No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT24450.12408207178997906No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA23760.12058036915050725No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT21250.10784229143300837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20390.1034778504620725No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT20370.10337635183484144No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT19900.10099113409491137No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT19820.1005851395859871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT502.798832E-522.40008710
TAGGACC39750.022.3340444
CAGTGCG851.0591975E-820.7033377
GCGCATT555.8358197E-520.36371811
CTCGCCT555.8358197E-520.36371831
GTCGAGA1400.019.42766419
AGGACCT71850.019.2575425
CGGTAGG1500.019.2210541
GTGCGCA507.199472E-419.1995899
TGTAGGA131350.019.1079482
GGACCTG70400.018.9725326
CTGTAGG130300.018.7096751
TCGCCTT601.13867194E-418.6667432
GTGTAGG3650.018.4311491
GTATCAA35050.018.3253521
TCCGGTG701.788253E-518.28578812
GTAGGAC135550.018.093863
GTCCTAC125350.017.8384481
ATGGCGC450.00885484117.77694517
AGTGCGC450.00885872517.7755938
Produced by FastQC (version 0.11.5)