##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062896_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970470
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36958745882962	32.0	32.0	32.0	32.0	32.0
2	31.12167706181774	32.0	32.0	32.0	32.0	32.0
3	31.145007536273074	32.0	32.0	32.0	32.0	32.0
4	31.19679467335204	32.0	32.0	32.0	32.0	32.0
5	31.19116657447208	32.0	32.0	32.0	32.0	32.0
6	34.86242267073338	36.0	36.0	36.0	36.0	36.0
7	34.80303988388557	36.0	36.0	36.0	32.0	36.0
8	34.79178064116683	36.0	36.0	36.0	32.0	36.0
9	34.87630666795231	36.0	36.0	36.0	36.0	36.0
10	34.67938156886428	36.0	36.0	36.0	32.0	36.0
11	34.85405613889072	36.0	36.0	36.0	36.0	36.0
12	34.72086405781362	36.0	36.0	36.0	32.0	36.0
13	34.77179048653367	36.0	36.0	36.0	32.0	36.0
14	34.738171096235924	36.0	36.0	36.0	32.0	36.0
15	34.706986150512314	36.0	36.0	36.0	32.0	36.0
16	34.75377346521388	36.0	36.0	36.0	32.0	36.0
17	34.73506219328384	36.0	36.0	36.0	32.0	36.0
18	34.771904164996165	36.0	36.0	36.0	32.0	36.0
19	34.660067902581616	36.0	36.0	36.0	32.0	36.0
20	34.629042817195895	36.0	36.0	36.0	32.0	36.0
21	34.59237846808122	36.0	36.0	36.0	32.0	36.0
22	34.54933797520388	36.0	36.0	36.0	32.0	36.0
23	34.57971296188219	36.0	36.0	36.0	32.0	36.0
24	34.530635330657155	36.0	36.0	36.0	32.0	36.0
25	34.50864007064305	36.0	36.0	36.0	32.0	36.0
26	34.53605941729638	36.0	36.0	36.0	32.0	36.0
27	34.46499769090623	36.0	36.0	36.0	32.0	36.0
28	34.36497383872883	36.0	36.0	36.0	32.0	36.0
29	34.27252229163601	36.0	36.0	36.0	32.0	36.0
30	34.21576476678153	36.0	36.0	36.0	32.0	36.0
31	34.203298197891876	36.0	36.0	36.0	32.0	36.0
32	34.179680989814614	36.0	36.0	36.0	32.0	36.0
33	34.25930920034307	36.0	36.0	36.0	32.0	36.0
34	34.22502347155755	36.0	36.0	36.0	32.0	36.0
35	34.23610712165118	36.0	36.0	36.0	32.0	36.0
36	34.14078925332535	36.0	36.0	36.0	32.0	36.0
37	34.018043918456	36.0	36.0	36.0	32.0	36.0
38	33.44910402086812	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	9.0
8	6.0
9	2.0
10	14.0
11	19.0
12	12.0
13	11.0
14	113.0
15	289.0
16	437.0
17	550.0
18	632.0
19	742.0
20	997.0
21	1433.0
22	2119.0
23	3208.0
24	5034.0
25	8212.0
26	12663.0
27	19525.0
28	29059.0
29	41122.0
30	57618.0
31	80943.0
32	112457.0
33	176387.0
34	442736.0
35	974117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63317445960915	20.72844788162722	12.565065395676871	26.073312263086756
2	10.212174114392438	19.69937938157522	52.08477418162356	18.003672322408786
3	19.577437409178785	25.82720583570236	29.777024561253914	24.81833219386494
4	9.736028619526056	17.223227938650112	42.06195382365942	30.978789618164406
5	9.10328272768103	42.09691621013314	38.35348509108256	10.446315971103267
6	29.066098137291522	40.91462721470679	19.11184370787794	10.907430940123747
7	26.641806231720704	31.717405061845742	22.914314220943563	18.726474485489987
8	20.168905237067193	47.9542785571244	19.664605212808826	12.212210992999582
9	26.890001882808136	14.782276837755226	21.73019410178633	36.597527177650306
10	15.213311463562675	29.409495892313032	37.082100959576785	18.295091684547504
11	32.98393235959846	19.964068574212604	31.489073395519735	15.562925670669198
12	27.764382390899776	22.877488120510776	35.45226305348168	13.905866435107766
13	31.755557112098497	24.50276066426485	26.08525727117042	17.656424952466224
14	16.466551793268486	28.641744722210237	27.269963482440357	27.62174000208092
15	17.829484909522108	44.59985461810857	21.535961713394627	16.03469875897469
16	15.631028785906251	28.13441036221567	39.555281476575125	16.679279375302947
17	16.310297778337265	34.22527075372477	33.28103363559208	16.183397832345882
18	16.559173183268776	26.975230821700897	42.3055880370614	14.160007957968931
19	24.374983187899144	25.25631792405955	27.832312426439128	22.536386461602177
20	24.06771402977651	29.924353349286548	31.475706031177282	14.532226589759661
21	18.76905758588348	28.06210489132862	26.804811748608365	26.364025774179535
22	21.845831212274547	36.48588224964359	27.220031659815835	14.44825487826603
23	16.404656795321685	35.434470801641595	32.12438625536852	16.036486147668203
24	24.038960808248973	27.13278194687301	34.08669744211838	14.74155980275964
25	18.79293830892792	32.324682266031104	33.5302922710917	15.35208715394927
26	13.70758525332991	34.626533996514894	35.25081734471212	16.415063405443075
27	15.868128990214124	33.95415596392205	32.54451504322463	17.633200002639207
28	14.861296427594448	34.813102017760265	28.454647466722964	21.870954087922318
29	18.896756982820577	29.94969202096775	27.53773098427419	23.615820011937487
30	15.50918947668653	30.593694903486895	32.63682713105827	21.260288488768307
31	23.40172851398448	27.24142708729305	30.23280481819847	19.124039580523995
32	17.228629794577035	33.6293251113395	31.23586210396762	17.906182990115845
33	15.121070579714631	34.39385026724398	35.508809078022246	14.976270075019146
34	17.362095559741206	33.06498807495395	34.895694548282094	14.677221817022742
35	15.583402406223717	34.777967668303944	33.74088453567536	15.897745389796974
36	14.439225487369606	31.689328016697054	36.79980590487715	17.071640591056195
37	15.852799421803374	31.48298908166621	29.087421787131063	23.57678970939935
38	17.639084708980047	37.08465416733914	28.135058512565813	17.141202611115002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	76.5
2	113.0
3	113.0
4	285.5
5	458.0
6	458.0
7	625.0
8	792.0
9	984.0
10	1176.0
11	1176.0
12	1584.5
13	1993.0
14	3085.0
15	4177.0
16	4177.0
17	6261.0
18	8345.0
19	8345.0
20	11692.0
21	15039.0
22	21693.0
23	28347.0
24	28347.0
25	44137.0
26	59927.0
27	59927.0
28	84454.5
29	108982.0
30	153255.5
31	197529.0
32	197529.0
33	245285.0
34	293041.0
35	293041.0
36	306558.5
37	320076.0
38	316586.5
39	313097.0
40	313097.0
41	264930.0
42	216763.0
43	174640.0
44	132517.0
45	132517.0
46	110165.0
47	87813.0
48	87813.0
49	75415.5
50	63018.0
51	52246.0
52	41474.0
53	41474.0
54	34826.0
55	28178.0
56	28178.0
57	22474.5
58	16771.0
59	13508.5
60	10246.0
61	10246.0
62	8576.0
63	6906.0
64	5869.5
65	4833.0
66	4833.0
67	4178.5
68	3524.0
69	3524.0
70	2769.5
71	2015.0
72	1557.0
73	1099.0
74	1099.0
75	875.0
76	651.0
77	651.0
78	476.5
79	302.0
80	233.0
81	164.0
82	164.0
83	139.5
84	115.0
85	115.0
86	90.5
87	66.0
88	56.0
89	46.0
90	46.0
91	37.0
92	28.0
93	27.0
94	26.0
95	26.0
96	36.0
97	46.0
98	46.0
99	391.5
100	737.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12433581835805671
2	0.05805721477617015
3	0.012839576344729936
4	0.003400204012240735
5	2.537465680776668E-4
6	3.044958816932001E-4
7	1.5224794084660006E-4
8	8.119890178485336E-4
9	4.567438225398002E-4
10	9.134876450796003E-4
11	0.0025374656807766673
12	0.002892710876085401
13	0.01481879957573574
14	0.00938862301887367
15	0.02466416641714921
16	0.011926088699650337
17	0.021010215836830808
18	0.0059884190066329356
19	0.008272138119331937
20	0.006343664201941668
21	0.006394413515557202
22	0.007561647728714469
23	0.00954087095972027
24	0.014463554380427004
25	0.01761001182459007
26	0.018827995351362873
27	0.009185625764411537
28	0.005937669693017402
29	0.01045435860479987
30	0.0032479560713941343
31	0.005430176556862069
32	0.007358650474252336
33	0.00877963125548727
34	0.012535080463036736
35	0.016696524179510473
36	0.01532629271189107
37	0.01045435860479987
38	0.006292914888326136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1970470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.51796917702594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82666303921779	37.932009138023865
2	12.004792955205783	11.408867632440929
3	3.42811728333308	4.886915142139536
4	1.4216682786664476	2.7021915778251113
5	0.7278938933187022	1.7294019793431747
6	0.46426064444783693	1.3236433795787108
7	0.32017537175322514	1.06498584123488
8	0.24347843464422206	0.9255680602155736
9	0.18359381450240947	0.7851604696756298
>10	1.1455859962210933	10.526938019983549
>50	0.12081021479728109	4.010336845603959
>100	0.09660991657754667	9.139763247755939
>500	0.009404012050180398	3.204998597389052
>1k	0.006091235077957758	6.010057893285041
>5k	4.274550931900181E-4	1.3169154196192356
>10k+	4.274550931900181E-4	3.0322467558858266
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	18628	0.9453582140301552	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	15189	0.7708313245063361	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	13778	0.6992240429948184	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	12119	0.6150309317066487	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	7332	0.3720939674290905	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	7025	0.35651392814912175	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6569	0.33337224114043856	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	5008	0.254152562586591	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	4843	0.245778925840028	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	4248	0.21558308423878567	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	3923	0.19908955731373734	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	3893	0.19756707790527134	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	3567	0.18102280166660747	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	3349	0.1699594512984212	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3340	0.1695027074758814	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	3314	0.16818322532187752	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	3224	0.1636157870964795	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	3142	0.15945434338000578	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	3062	0.15539439829076312	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	2965	0.1504717148700564	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	2931	0.14874623820712826	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	2929	0.14864473957989718	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	2866	0.14544753282211859	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	2700	0.13702314676194005	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2624	0.1331661989271595	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	2462	0.12494481012144312	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	2454	0.12453881561251884	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	2445	0.12408207178997906	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	2376	0.12058036915050725	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	2125	0.10784229143300837	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2039	0.1034778504620725	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	2037	0.10337635183484144	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	1990	0.10099113409491137	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	1982	0.1005851395859871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	5.074931361553335E-5	0.0
9	0.0	0.0	0.0	5.074931361553335E-5	0.0
10	0.0	0.0	0.0	1.5224794084660006E-4	0.0
11	0.0	0.0	0.0	1.5224794084660006E-4	0.0
12	0.0	0.0	0.0	1.5224794084660006E-4	0.0
13	0.0	0.0	0.0	1.5224794084660006E-4	0.0
14	0.0	0.0	0.0	1.5224794084660006E-4	0.0
15	0.0	0.0	0.0	1.5224794084660006E-4	0.0
16	0.0	0.0	0.0	1.5224794084660006E-4	0.0
17	0.0	0.0	0.0	1.5224794084660006E-4	0.0
18	0.0	5.074931361553335E-5	0.0	2.029972544621334E-4	0.0
19	0.0	5.074931361553335E-5	0.0	2.5374656807766673E-4	0.0
20	0.0	5.074931361553335E-5	0.0	2.5374656807766673E-4	0.0
21	0.0	5.074931361553335E-5	0.0	3.044958816932001E-4	0.0
22	0.0	5.074931361553335E-5	0.0	3.044958816932001E-4	0.0
23	0.0	5.074931361553335E-5	0.0	5.074931361553335E-4	0.0
24	0.0	5.074931361553335E-5	0.0	7.612397042330003E-4	0.0
25	0.0	5.074931361553335E-5	0.0	8.119890178485336E-4	0.0
26	0.0	5.074931361553335E-5	0.0	0.0010657355859262003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCAT	50	2.798832E-5	22.400087	10
TAGGACC	3975	0.0	22.334044	4
CAGTGCG	85	1.0591975E-8	20.703337	7
GCGCATT	55	5.8358197E-5	20.363718	11
CTCGCCT	55	5.8358197E-5	20.363718	31
GTCGAGA	140	0.0	19.427664	19
AGGACCT	7185	0.0	19.257542	5
CGGTAGG	150	0.0	19.221054	1
GTGCGCA	50	7.199472E-4	19.199589	9
TGTAGGA	13135	0.0	19.107948	2
GGACCTG	7040	0.0	18.972532	6
CTGTAGG	13030	0.0	18.709675	1
TCGCCTT	60	1.13867194E-4	18.66674	32
GTGTAGG	365	0.0	18.431149	1
GTATCAA	3505	0.0	18.325352	1
TCCGGTG	70	1.788253E-5	18.285788	12
GTAGGAC	13555	0.0	18.09386	3
GTCCTAC	12535	0.0	17.838448	1
ATGGCGC	45	0.008854841	17.776945	17
AGTGCGC	45	0.008858725	17.775593	8
>>END_MODULE