FastQCFastQC Report
Thu 2 Feb 2017
SRR4062896_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062896_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970470
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA190460.9665714271214482No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC145410.7379457692834704No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA130450.6620247961146326No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG126290.6409130816505707No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA74520.37818388506295453No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA69580.353113724136881No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA68420.3472268037574792No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA55300.2806437042938994No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA45930.2330915974361447No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA43880.22268798814496035No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT40210.20406299004805958No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA40050.20325100103021107No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG38290.1943191218338772No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG36550.1854887412647744No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG35470.18000781539429678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34150.17330890599704638No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT33630.17066994168903865No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT33300.16899521433972606No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA33260.16879221708526393No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT31970.16224555562886012No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA31750.1611290707293184No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT30430.154430161332068No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC29170.14803574781651077No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT27540.13976360969717885No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA27420.13915461793379244No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA27230.1381903809750973No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA25980.13184671677315563No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA24750.12560455119844505No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT24390.12377757590828584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23650.12002212670073636No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT23210.11778915690165291No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT22750.11545468847533837No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT22380.11357696387156364No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA20970.10642131065177343No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT20880.10596456682923364No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG20580.10444208742076765No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA20140.10220911762168416No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC20060.10180312311275991No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT19960.10129562997660457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC41150.023.9533734
ACGGCGA1005.456968E-1222.39900617
TACGGCG957.2759576E-1121.89376316
CGGCGAG1101.8189894E-1221.81721118
GACGTGC555.8288824E-520.3668657
AGGACCT72800.020.1130815
TATTACG400.004484432320.0001282
GCGACGA400.00448574919.99911320
GGACCTG71550.019.7934956
GTGTAGG4000.019.5996281
GACGTTG902.0369043E-819.5586597
TGTAGGA134300.019.3955062
CTGTAGG131900.019.0928881
CCTACCG1550.018.5821783
GTAGGAC137950.018.5241813
GCGATAA1055.988113E-918.28490420
TGGCGAC1251.4915713E-1017.91920518
TTTAGAA33950.017.8616752
GGCGAAA3050.017.83527219
AATGTCC30750.017.7974938
Produced by FastQC (version 0.11.5)