##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970470
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.337796566301442	32.0	32.0	32.0	32.0	32.0
2	31.419480124031324	32.0	32.0	32.0	32.0	32.0
3	31.473532710470092	32.0	32.0	32.0	32.0	32.0
4	31.545952488492592	32.0	32.0	32.0	32.0	32.0
5	31.521328921526337	32.0	32.0	32.0	32.0	32.0
6	35.06173400254762	36.0	36.0	36.0	36.0	36.0
7	35.099758940760324	36.0	36.0	36.0	36.0	36.0
8	35.00967434165453	36.0	36.0	36.0	36.0	36.0
9	35.14280603104843	36.0	36.0	36.0	36.0	36.0
10	35.02429420392089	36.0	36.0	36.0	36.0	36.0
11	35.15190081554147	36.0	36.0	36.0	36.0	36.0
12	35.0978776637046	36.0	36.0	36.0	36.0	36.0
13	35.12484584895989	36.0	36.0	36.0	36.0	36.0
14	35.09254137337793	36.0	36.0	36.0	36.0	36.0
15	35.0651671936137	36.0	36.0	36.0	36.0	36.0
16	35.09591569524022	36.0	36.0	36.0	36.0	36.0
17	35.118667120027204	36.0	36.0	36.0	36.0	36.0
18	35.12271539277431	36.0	36.0	36.0	36.0	36.0
19	35.06465817799814	36.0	36.0	36.0	36.0	36.0
20	35.049574467005336	36.0	36.0	36.0	36.0	36.0
21	35.01585154810781	36.0	36.0	36.0	36.0	36.0
22	34.98690160215583	36.0	36.0	36.0	36.0	36.0
23	34.986722964571904	36.0	36.0	36.0	36.0	36.0
24	34.97324749932757	36.0	36.0	36.0	36.0	36.0
25	34.95014844174233	36.0	36.0	36.0	36.0	36.0
26	34.89855262957568	36.0	36.0	36.0	36.0	36.0
27	34.826031860419086	36.0	36.0	36.0	32.0	36.0
28	34.76206387308611	36.0	36.0	36.0	32.0	36.0
29	34.73073327683243	36.0	36.0	36.0	32.0	36.0
30	34.658360188178456	36.0	36.0	36.0	32.0	36.0
31	34.6475668241587	36.0	36.0	36.0	32.0	36.0
32	34.63577420615386	36.0	36.0	36.0	32.0	36.0
33	34.69041802209625	36.0	36.0	36.0	32.0	36.0
34	34.66991732936812	36.0	36.0	36.0	32.0	36.0
35	34.66338690769207	36.0	36.0	36.0	32.0	36.0
36	34.5817160372906	36.0	36.0	36.0	32.0	36.0
37	34.475105939192176	36.0	36.0	36.0	32.0	36.0
38	34.13758291169112	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	20.0
22	64.0
23	233.0
24	655.0
25	1864.0
26	4299.0
27	8756.0
28	16928.0
29	28618.0
30	45702.0
31	69417.0
32	101497.0
33	165534.0
34	440114.0
35	1086766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.25636304207921	21.952622058545877	13.121052599523725	26.669962299851193
2	9.802635919349191	20.774130029891346	50.88613376504083	18.537100285718637
3	19.053119306561378	26.652930519114733	30.18858444939532	24.10536572492857
4	9.578744531118765	18.133467853052093	42.237043299887794	30.050744315941348
5	8.868442554314452	42.79618568158865	37.624779874852194	10.710591889244698
6	29.443614410383727	40.95920360036296	18.71651810635849	10.880663882894826
7	26.777618818762534	32.03299332795049	22.83376614281241	18.355621710474566
8	20.322316251570093	48.096121395130496	19.35222096755776	12.229341385741655
9	26.227434047354052	14.835431140316821	21.688740965625605	37.24839384670352
10	15.215825786430038	29.92466207043551	36.351705570540815	18.50780657259364
11	32.55913685991635	20.101330347794622	31.74391629664629	15.595616495642734
12	27.670611926315907	23.079007706806408	35.21618743992538	14.034192926952304
13	31.63757626999082	24.50525687565435	26.139285763222624	17.717881091132202
14	16.539607301811245	28.866920074905988	27.113683537430155	27.479789085852612
15	17.821078219917077	44.681928676914644	21.37221069085041	16.124782412317874
16	15.792644184021256	28.61558776899701	38.64472805445204	16.947039992529696
17	16.387156363710183	34.84595045852005	32.497373723020395	16.269519454749375
18	16.654655995777656	27.380472679107015	41.68766842428456	14.277202900830765
19	24.67873147015687	25.393028059295496	27.96282105284526	21.965419417702375
20	24.417981496800255	30.3835633123062	30.77565250929981	14.422802681593733
21	18.89931731954033	27.970563338252642	26.97531753877759	26.15480180342944
22	22.136608086704232	36.15601158911914	27.21854543258483	14.488834891591798
23	16.37362291597732	35.674316964294775	31.737637962149094	16.21442215757881
24	24.27451318720914	27.52881292280522	33.34590224667212	14.850771643313523
25	18.963749765284422	32.7443706323872	32.753404010210765	15.538475592117617
26	13.820770588118869	34.988269290204514	34.51533619661106	16.675623925065555
27	16.17499810958519	34.31359165111621	31.988203811583755	17.52320642771485
28	15.106294437367735	35.042096555644086	28.33004308616726	21.52156592082092
29	18.89300522210437	29.875511933701098	27.618131714768555	23.613351129425975
30	15.669561069186539	30.44197577227768	32.46073271858998	21.4277304399458
31	23.035113450090588	27.407826559145786	30.37163722360655	19.185422767157075
32	17.37529624911823	33.61304663354428	31.0382802072602	17.97337691007729
33	15.235908184341806	34.647926636792235	34.940191933904096	15.175973244961863
34	17.692530208528932	33.345394753535956	34.16560516019021	14.796469877744903
35	15.905291630930693	35.145929651301465	32.86520474810578	16.08357396966206
36	14.565915745989535	31.978867985810496	36.15802321273605	17.297193055463925
37	15.9845701708578	31.588165051061445	28.698497667306615	23.728767110774136
38	17.936500364380443	36.667380541069434	28.198478737031003	17.197640357519127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	26.0
2	36.0
3	36.0
4	171.0
5	306.0
6	306.0
7	485.5
8	665.0
9	846.5
10	1028.0
11	1028.0
12	1512.0
13	1996.0
14	2997.5
15	3999.0
16	3999.0
17	6146.5
18	8294.0
19	8294.0
20	11511.0
21	14728.0
22	21065.0
23	27402.0
24	27402.0
25	43091.0
26	58780.0
27	58780.0
28	82896.0
29	107012.0
30	150207.5
31	193403.0
32	193403.0
33	242479.5
34	291556.0
35	291556.0
36	305995.5
37	320435.0
38	319395.5
39	318356.0
40	318356.0
41	270192.0
42	222028.0
43	178288.5
44	134549.0
45	134549.0
46	111490.0
47	88431.0
48	88431.0
49	75480.0
50	62529.0
51	51718.5
52	40908.0
53	40908.0
54	34506.5
55	28105.0
56	28105.0
57	22370.0
58	16635.0
59	13314.5
60	9994.0
61	9994.0
62	8493.5
63	6993.0
64	5844.0
65	4695.0
66	4695.0
67	4063.0
68	3431.0
69	3431.0
70	2696.0
71	1961.0
72	1464.0
73	967.0
74	967.0
75	783.5
76	600.0
77	600.0
78	447.0
79	294.0
80	225.0
81	156.0
82	156.0
83	121.5
84	87.0
85	87.0
86	68.0
87	49.0
88	40.0
89	31.0
90	31.0
91	20.0
92	9.0
93	6.0
94	3.0
95	3.0
96	2.5
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009439372332489204
2	0.0
3	0.0
4	1.5224794084660006E-4
5	0.0
6	1.014986272310667E-4
7	5.582424497708668E-4
8	0.0022837191126990008
9	0.004009195775627135
10	7.612397042330003E-4
11	0.004212193030089268
12	2.029972544621334E-4
13	1.5224794084660006E-4
14	0.0
15	0.0
16	1.014986272310667E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.014986272310667E-4
22	5.074931361553335E-5
23	1.014986272310667E-4
24	0.0
25	0.0
26	5.074931361553335E-5
27	1.5224794084660006E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	5.074931361553335E-5
38	1.014986272310667E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1970470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.858010837524276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49657057155815	35.99696583979003
2	12.7485356897273	11.692449756441592
3	3.6881851165636395	5.0739849913851325
4	1.4764060379032986	2.7082017634702296
5	0.8260592937911546	1.8940718023556204
6	0.5047878286100829	1.388913942903092
7	0.3497957625967661	1.1228656510457797
8	0.24696110199479987	0.9060115913379576
9	0.20392342944701025	0.8416370553845501
>10	1.2079398245815192	10.680962351619083
>50	0.13079914444228158	4.194344470250838
>100	0.10238122070687766	9.247185308456578
>500	0.009993383817232724	3.312373092499552
>1k	0.006773293476124402	6.55875433971034
>5k	4.4415039187700994E-4	1.363729630033712
>10k+	4.4415039187700994E-4	3.017548413315952
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	19046	0.9665714271214482	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	14541	0.7379457692834704	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	13045	0.6620247961146326	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	12629	0.6409130816505707	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	7452	0.37818388506295453	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6958	0.353113724136881	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	6842	0.3472268037574792	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	5530	0.2806437042938994	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	4593	0.2330915974361447	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	4388	0.22268798814496035	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	4021	0.20406299004805958	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	4005	0.20325100103021107	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	3829	0.1943191218338772	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	3655	0.1854887412647744	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	3547	0.18000781539429678	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3415	0.17330890599704638	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	3363	0.17066994168903865	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	3330	0.16899521433972606	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	3326	0.16879221708526393	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	3197	0.16224555562886012	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	3175	0.1611290707293184	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	3043	0.154430161332068	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	2917	0.14803574781651077	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	2754	0.13976360969717885	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	2742	0.13915461793379244	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2723	0.1381903809750973	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	2598	0.13184671677315563	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	2475	0.12560455119844505	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	2439	0.12377757590828584	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2365	0.12002212670073636	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	2321	0.11778915690165291	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	2275	0.11545468847533837	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	2238	0.11357696387156364	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	2097	0.10642131065177343	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	2088	0.10596456682923364	No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2058	0.10444208742076765	No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2014	0.10220911762168416	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	2006	0.10180312311275991	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	1996	0.10129562997660457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.074931361553335E-5	0.0
11	0.0	0.0	0.0	5.074931361553335E-5	0.0
12	0.0	0.0	0.0	5.074931361553335E-5	0.0
13	0.0	0.0	0.0	5.074931361553335E-5	0.0
14	0.0	0.0	0.0	5.074931361553335E-5	0.0
15	0.0	0.0	0.0	5.074931361553335E-5	0.0
16	0.0	0.0	0.0	5.074931361553335E-5	0.0
17	0.0	0.0	0.0	5.074931361553335E-5	0.0
18	0.0	5.074931361553335E-5	0.0	1.014986272310667E-4	0.0
19	0.0	5.074931361553335E-5	0.0	1.014986272310667E-4	0.0
20	0.0	5.074931361553335E-5	0.0	1.014986272310667E-4	0.0
21	0.0	5.074931361553335E-5	0.0	2.029972544621334E-4	0.0
22	0.0	5.074931361553335E-5	0.0	2.029972544621334E-4	0.0
23	0.0	5.074931361553335E-5	0.0	3.5524519530873345E-4	0.0
24	0.0	5.074931361553335E-5	0.0	6.089917633864002E-4	0.0
25	0.0	5.074931361553335E-5	0.0	6.597410770019335E-4	0.0
26	0.0	5.074931361553335E-5	0.0	8.62738331464067E-4	5.074931361553335E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	4115	0.0	23.953373	4
ACGGCGA	100	5.456968E-12	22.399006	17
TACGGCG	95	7.2759576E-11	21.893763	16
CGGCGAG	110	1.8189894E-12	21.817211	18
GACGTGC	55	5.8288824E-5	20.366865	7
AGGACCT	7280	0.0	20.113081	5
TATTACG	40	0.0044844323	20.000128	2
GCGACGA	40	0.004485749	19.999113	20
GGACCTG	7155	0.0	19.793495	6
GTGTAGG	400	0.0	19.599628	1
GACGTTG	90	2.0369043E-8	19.558659	7
TGTAGGA	13430	0.0	19.395506	2
CTGTAGG	13190	0.0	19.092888	1
CCTACCG	155	0.0	18.582178	3
GTAGGAC	13795	0.0	18.524181	3
GCGATAA	105	5.988113E-9	18.284904	20
TGGCGAC	125	1.4915713E-10	17.919205	18
TTTAGAA	3395	0.0	17.861675	2
GGCGAAA	305	0.0	17.835272	19
AATGTCC	3075	0.0	17.797493	8
>>END_MODULE