FastQCFastQC Report
Thu 2 Feb 2017
SRR4062895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354348
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA62530.46169817506283467No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA60170.44427281614474273No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG54510.40248148924796284No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC52790.38978165139240434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40480.2988892072052382No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA30390.22438841420373493No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA30230.2232070339381016No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA26300.19418938116348233No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA26070.19249114703163442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24000.1772070398450029No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA21350.15764042919545052No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT20510.1514381828008754No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19550.14434990120707528No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT15500.11444621323323104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14790.10920383830448305No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA14720.10868698443826845No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG14510.10713642283962468No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT14260.10529051617457255No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14040.1036661183093267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC350.002066729422.85714310
GGACGTA703.0911906E-822.8529246
TAGGACC20400.021.7222824
GTGCGCC453.536336E-421.33412211
AGGACCT30850.020.0157555
TATCGCG400.00448388620.016
GCGACAA400.004484844419.99926220
TCACGGT400.00448580319.99852227
GACGTAA400.004487720319.9970467
TAAGTCG400.004488679619.9963075
GGACCTG30400.019.73326
ACGAACG1154.5474735E-1119.4789815
CGAACGA1154.5474735E-1119.4782616
GTCCTAG2900.019.3324761
CGGTAGG507.1415934E-419.2220041
GTGCGTT507.195057E-419.20070811
CTTCGCC507.1986334E-419.19929130
TTCACGG507.1986334E-419.19929126
TTCGCCA507.1986334E-419.19929131
CACGGTT507.200422E-419.19858228
Produced by FastQC (version 0.11.5)