##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062895_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1354348 Sequences flagged as poor quality 0 Sequence length 38 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26276259868217 32.0 32.0 32.0 32.0 32.0 2 30.954584050775726 32.0 32.0 32.0 32.0 32.0 3 31.000913354617868 32.0 32.0 32.0 32.0 32.0 4 31.045054889880593 32.0 32.0 32.0 32.0 32.0 5 31.022028311778065 32.0 32.0 32.0 32.0 32.0 6 34.67596806729142 36.0 36.0 36.0 32.0 36.0 7 34.59153629643193 36.0 36.0 36.0 32.0 36.0 8 34.58676721197211 36.0 36.0 36.0 32.0 36.0 9 34.67203776282019 36.0 36.0 36.0 32.0 36.0 10 34.454109283581474 36.0 36.0 36.0 32.0 36.0 11 34.66390617477931 36.0 36.0 36.0 32.0 36.0 12 34.51934953202574 36.0 36.0 36.0 32.0 36.0 13 34.58871058250907 36.0 36.0 36.0 32.0 36.0 14 34.5181053909335 36.0 36.0 36.0 32.0 36.0 15 34.48482886230127 36.0 36.0 36.0 32.0 36.0 16 34.51326911547106 36.0 36.0 36.0 32.0 36.0 17 34.46543576687823 36.0 36.0 36.0 32.0 36.0 18 34.51152584121659 36.0 36.0 36.0 32.0 36.0 19 34.429981068381245 36.0 36.0 36.0 32.0 36.0 20 34.41645426433974 36.0 36.0 36.0 32.0 36.0 21 34.377228747707385 36.0 36.0 36.0 32.0 36.0 22 34.35096666440236 36.0 36.0 36.0 32.0 36.0 23 34.35097626311701 36.0 36.0 36.0 32.0 36.0 24 34.320461210855704 36.0 36.0 36.0 32.0 36.0 25 34.304426927200396 36.0 36.0 36.0 32.0 36.0 26 34.29307607793566 36.0 36.0 36.0 32.0 36.0 27 34.251191717342955 36.0 36.0 36.0 32.0 36.0 28 34.188360746277915 36.0 36.0 36.0 32.0 36.0 29 34.12560213475414 36.0 36.0 36.0 32.0 36.0 30 34.080409171055 36.0 36.0 36.0 32.0 36.0 31 34.088801401116996 36.0 36.0 36.0 32.0 36.0 32 34.06312114759279 36.0 36.0 36.0 32.0 36.0 33 34.07987976502346 36.0 36.0 36.0 32.0 36.0 34 34.05143212822701 36.0 36.0 36.0 32.0 36.0 35 34.045293381021715 36.0 36.0 36.0 32.0 36.0 36 33.964912267748026 36.0 36.0 36.0 32.0 36.0 37 33.90478444240328 36.0 36.0 36.0 32.0 36.0 38 33.33575491675699 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 0.0 9 5.0 10 10.0 11 12.0 12 4.0 13 5.0 14 89.0 15 246.0 16 333.0 17 437.0 18 494.0 19 672.0 20 949.0 21 1363.0 22 2032.0 23 3178.0 24 5060.0 25 7783.0 26 11625.0 27 16888.0 28 24277.0 29 32936.0 30 45303.0 31 60846.0 32 83243.0 33 126674.0 34 294248.0 35 635632.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.825968731771475 20.480268279537697 12.668977738742395 25.024785249948433 2 12.277060946463598 20.976015189518048 46.98924691462915 19.757676949389204 3 18.05422018606066 27.624056441453217 30.192839662615512 24.128883709870607 4 10.479890837239388 17.56284390256869 40.58596715075153 31.371298109440392 5 10.613986834964503 40.58500603612816 36.34472752511362 12.456279603793716 6 28.554214109719624 39.78992027868863 19.8253322828855 11.830533328706244 7 26.41861619022585 32.556846541656945 23.65603227530886 17.368504992808347 8 23.007760958634556 41.448879641272555 20.736622849603094 14.806736550489793 9 27.50948060463221 14.753120329842345 21.636526613622536 36.100872451902916 10 15.473565761085457 28.658418626905167 34.73059242052598 21.137423191483393 11 33.53852856045839 20.97657865201725 28.665012700100416 16.819880087423947 12 26.18927017140731 24.155405155953034 33.27829948357771 16.377025189061946 13 29.733585689821577 23.28032911697137 26.64120947976213 20.344875713444925 14 19.23278284210021 25.67740696845844 27.485720825995298 27.604089363446054 15 19.82448249810015 38.57618571798888 22.78492791641095 18.814403867500015 16 18.716844480676755 28.081691064402936 33.786951050933965 19.41451340398634 17 18.65617797300481 31.772485346279762 30.38232972181379 19.18900695890164 18 19.3769262917877 26.329519761704688 36.89767154057562 17.39588240593198 19 24.086432953394198 25.586818406027827 28.299681300082995 22.027067340494973 20 24.65044156304457 27.624863396048084 29.74333225034705 17.981362790560297 21 22.30395541217378 26.561891969923302 26.715037093757456 24.41911552414546 22 23.613395054572198 31.815875964825096 27.329886927261438 17.240842053341268 23 18.270428571534058 31.323403554605783 30.912546714289828 19.493621159570328 24 23.248869058959578 27.086536373988135 31.61908812847904 18.04550643857325 25 20.224482622129536 29.40864137723242 31.38621231545428 18.980663685183764 26 17.255897042413807 31.476075447286078 32.410858489444365 18.85716902085575 27 19.33816704436957 30.54239318170574 30.06404334833069 20.055396425594004 28 17.77673909624369 31.017713458285247 28.748686572432163 22.456860873038902 29 20.673162482785106 28.764478051698227 27.90892073209005 22.653438733426622 30 18.140119750751857 29.048676339959815 30.858154651103547 21.953049258184777 31 23.09825146941548 27.07704462888029 29.04111409752784 20.78358980417639 32 19.507101949676432 30.520809642938367 29.576393585945553 20.395694821439648 33 17.569972412733854 31.049113604007538 32.69430709884748 18.686606884411127 34 19.948692727925245 30.16820337411036 32.08402931928512 17.799074578679274 35 19.303760778814336 30.96271418243473 31.357054294134894 18.37647074461604 36 17.59928457652439 29.997570454959998 32.70499932430586 19.698145644209745 37 18.978048829115817 29.998825903666503 28.167678678632114 22.85544658858557 38 19.58661337377804 32.614450367243926 28.334897513581048 19.46403874539699 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 272.0 1 238.0 2 204.0 3 204.0 4 509.5 5 815.0 6 815.0 7 1015.5 8 1216.0 9 1156.5 10 1097.0 11 1097.0 12 1534.0 13 1971.0 14 2757.5 15 3544.0 16 3544.0 17 5330.5 18 7117.0 19 7117.0 20 9575.5 21 12034.0 22 14851.5 23 17669.0 24 17669.0 25 25873.0 26 34077.0 27 34077.0 28 47429.5 29 60782.0 30 84458.5 31 108135.0 32 108135.0 33 135161.0 34 162187.0 35 162187.0 36 169261.0 37 176335.0 38 174972.5 39 173610.0 40 173610.0 41 152179.0 42 130748.0 43 114154.5 44 97561.0 45 97561.0 46 85029.0 47 72497.0 48 72497.0 49 67460.0 50 62423.0 51 57226.5 52 52030.0 53 52030.0 54 45020.0 55 38010.0 56 38010.0 57 36279.5 58 34549.0 59 29644.5 60 24740.0 61 24740.0 62 25762.0 63 26784.0 64 21745.0 65 16706.0 66 16706.0 67 14601.5 68 12497.0 69 12497.0 70 10339.5 71 8182.0 72 6487.0 73 4792.0 74 4792.0 75 3603.0 76 2414.0 77 2414.0 78 2703.0 79 2992.0 80 2401.5 81 1811.0 82 1811.0 83 2008.0 84 2205.0 85 2205.0 86 1469.5 87 734.0 88 625.5 89 517.0 90 517.0 91 373.0 92 229.0 93 203.0 94 177.0 95 177.0 96 156.0 97 135.0 98 135.0 99 342.5 100 550.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12766290495500418 2 0.057813796749432204 3 0.012256820255946035 4 0.002953450664083382 5 2.2150879980625365E-4 6 3.6918133301042273E-4 7 0.0 8 3.6918133301042273E-4 9 2.9534506640833816E-4 10 4.430175996125073E-4 11 0.002067415464858367 12 0.0022150879980625364 13 0.013216691721773133 14 0.007605135460014708 15 0.02178169864761494 16 0.009155697058658483 17 0.018828247983531558 18 0.004577848529329242 19 0.007088281593800117 20 0.005020866128941749 21 0.00568539252836051 22 0.006423755194381356 23 0.007752807993218877 24 0.012256820255946035 25 0.01565328851964192 26 0.017499195184694036 27 0.008048153059627215 28 0.0049470298623396644 29 0.01055858612409809 30 0.002584269331072959 31 0.005020866128941749 32 0.005316211195350088 33 0.007383626660208454 34 0.01137078505672102 35 0.013807381854589811 36 0.013659709321385641 37 0.008491170659239723 38 0.005242374928748002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 1354348.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.00527287717194 #Duplication Level Percentage of deduplicated Percentage of total 1 84.57328622169061 45.674034005215674 2 9.862760437375803 10.652821374853119 3 2.316338495638571 3.7528347759857694 4 0.9274340314567695 2.00345311777594 5 0.4600936498775117 1.2423741555344516 6 0.3067403349328161 0.9939357294289112 7 0.22259331452892744 0.8414848884238222 8 0.15920764634963094 0.6878441908195286 9 0.12562063711318022 0.6105759107671342 >10 0.8277656681847958 8.88015310466759 >50 0.09944404552969821 3.763559147635954 >100 0.10457946077194466 11.74706241116466 >500 0.00837183931585101 3.1080894187086803 >1k 0.00521524416397276 4.337049943619459 >5k 5.489730698918694E-4 1.7047278253994287 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA 6253 0.46169817506283467 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA 6017 0.44427281614474273 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG 5451 0.40248148924796284 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC 5279 0.38978165139240434 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 4048 0.2988892072052382 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA 3039 0.22438841420373493 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA 3023 0.2232070339381016 No Hit ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA 2630 0.19418938116348233 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA 2607 0.19249114703163442 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 2400 0.1772070398450029 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA 2135 0.15764042919545052 No Hit TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT 2051 0.1514381828008754 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1955 0.14434990120707528 No Hit CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT 1550 0.11444621323323104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1479 0.10920383830448305 No Hit CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA 1472 0.10868698443826845 No Hit GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG 1451 0.10713642283962468 No Hit GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT 1426 0.10529051617457255 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 1404 0.1036661183093267 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 7.383626660208454E-5 0.0 18 0.0 0.0 0.0 2.2150879980625362E-4 0.0 19 0.0 0.0 0.0 2.9534506640833816E-4 0.0 20 0.0 0.0 0.0 2.9534506640833816E-4 0.0 21 0.0 0.0 0.0 4.4301759961250725E-4 0.0 22 0.0 0.0 0.0 5.168538662145919E-4 0.0 23 0.0 0.0 0.0 8.860351992250145E-4 0.0 24 0.0 0.0 0.0 0.0013290527988375218 0.0 25 0.0 0.0 0.0 0.0015505615986437754 0.0 26 0.0 0.0 0.0 0.0020674154648583675 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 35 0.0020667294 22.857143 10 GGACGTA 70 3.0911906E-8 22.852924 6 TAGGACC 2040 0.0 21.722282 4 GTGCGCC 45 3.536336E-4 21.334122 11 AGGACCT 3085 0.0 20.015755 5 TATCGCG 40 0.004483886 20.0 16 GCGACAA 40 0.0044848444 19.999262 20 TCACGGT 40 0.004485803 19.998522 27 GACGTAA 40 0.0044877203 19.997046 7 TAAGTCG 40 0.0044886796 19.996307 5 GGACCTG 3040 0.0 19.7332 6 ACGAACG 115 4.5474735E-11 19.47898 15 CGAACGA 115 4.5474735E-11 19.47826 16 GTCCTAG 290 0.0 19.332476 1 CGGTAGG 50 7.1415934E-4 19.222004 1 GTGCGTT 50 7.195057E-4 19.200708 11 CTTCGCC 50 7.1986334E-4 19.199291 30 TTCACGG 50 7.1986334E-4 19.199291 26 TTCGCCA 50 7.1986334E-4 19.199291 31 CACGGTT 50 7.200422E-4 19.198582 28 >>END_MODULE