FastQCFastQC Report
Thu 2 Feb 2017
SRR4062895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354348
Sequences flagged as poor quality0
Sequence length38
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA61660.45527441986845335No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA59550.4396949676154135No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG57650.4256660769610174No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC49740.3672615900787686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41040.303024038134955No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA33450.24698231178397279No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA30250.22335470647130576No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA27460.20275438808932417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26400.1949277438295032No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA25990.19190045689881774No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT22490.16605776358808816No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA21620.15963400839370678No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA18760.1385168361455106No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG16390.12101764096081656No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA16000.11813802656333526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15720.1160706110984769No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT15270.11274797910138311No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT14820.10942534710428929No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG14110.1041829721755413No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC13870.10241090177709126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA601.7636557E-724.0002779
CTAGGAC2900.020.9665323
GGAGGTC555.8320984E-520.3646248
TACCGTC1900.020.2115067
CGTGCAA400.00448358520.000239
TACGGCG400.00448454319.99949316
ACCGTCG1950.019.6932628
CGCTTCG1500.019.19951232
CTCTAGG3100.019.0984041
TAGGACC21700.019.09774
TCTAGGA3400.018.8244422
ATTTAGA17800.018.3386441
ATGTCCA17500.018.2859279
AATGTCC17700.018.0799718
AGGACCT32200.017.9891135
GGACCTG32300.017.933426
AAATGTC17950.017.9173037
TTTAGAA19450.017.8517652
TGTCCAC17300.017.84991610
TAGAACG904.3659384E-717.7786394
Produced by FastQC (version 0.11.5)