FastQCFastQC Report
Thu 2 Feb 2017
SRR4062894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541975
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32800.6051939665113705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22280.411089072374187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13040.24060150375939848No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12910.23820286913603028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.19428940449282714No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10480.1933668527146086No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8220.15166751233913003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7310.13487706997555238No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6250.11531897227731906No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6160.11365837907652566No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA6140.11328935836523825No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG5890.1086765994741455No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC5840.10775404769592693No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT5680.10480188200562757No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG5570.10277226809354675No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG5570.10277226809354675No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG5560.10258775773790303No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5430.1001891231145348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAT308.428823E-426.6570556
GGCGGGT703.079367E-822.8552332
GTCCTAT1550.020.6567781
CCTATAC400.0044898319.9927923
TAGTCAG400.0044898319.9927925
CCTATTC1800.019.5485083
TACCGTC1003.254172E-919.1983957
CATCGTT1257.2759576E-1219.19839328
CTATTCC1950.018.8649944
ATCGTTT1208.185452E-1118.66510429
ATACCGT1159.422365E-1018.0804396
CCCTATA450.00882986717.7828542
GCATTCG2250.017.77793322
CTATACC450.00886279317.7713724
TAAGGCT450.00886279317.7713724
ATTCGTA2350.017.70228224
GTAAACG1550.017.5469227
AAACGCT1601.8189894E-1216.99857929
AAGACGG1601.8189894E-1216.9938745
TCTTTCG1800.016.89059410
Produced by FastQC (version 0.11.5)