##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062894_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541975
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03326906222612	32.0	32.0	32.0	32.0	32.0
2	30.672881590479264	32.0	32.0	32.0	32.0	32.0
3	30.73641219613451	32.0	32.0	32.0	32.0	32.0
4	30.780379168780847	32.0	32.0	32.0	32.0	32.0
5	30.729799344988237	32.0	32.0	32.0	32.0	32.0
6	34.34541076617925	36.0	36.0	36.0	32.0	36.0
7	34.20881036948199	36.0	36.0	36.0	32.0	36.0
8	34.18094192536556	36.0	36.0	36.0	32.0	36.0
9	34.29375709211679	36.0	36.0	36.0	32.0	36.0
10	34.047253102080354	36.0	36.0	36.0	32.0	36.0
11	34.34128142441995	36.0	36.0	36.0	32.0	36.0
12	34.16277503574888	36.0	36.0	36.0	32.0	36.0
13	34.24345587896121	36.0	36.0	36.0	32.0	36.0
14	34.14737580146686	36.0	36.0	36.0	32.0	36.0
15	34.11366206928364	36.0	36.0	36.0	32.0	36.0
16	34.09574795885419	36.0	36.0	36.0	32.0	36.0
17	34.028962590525396	36.0	36.0	36.0	32.0	36.0
18	34.06389962636653	36.0	36.0	36.0	32.0	36.0
19	34.04046312099267	36.0	36.0	36.0	32.0	36.0
20	34.01200424373818	36.0	36.0	36.0	32.0	36.0
21	33.96666451404585	36.0	36.0	36.0	32.0	36.0
22	33.96270676691729	36.0	36.0	36.0	32.0	36.0
23	33.91011947045528	36.0	36.0	36.0	32.0	36.0
24	33.902539785045434	36.0	36.0	36.0	32.0	36.0
25	33.87226532589141	36.0	36.0	36.0	32.0	36.0
26	33.823338714885374	36.0	36.0	36.0	32.0	36.0
27	33.84727339821948	36.0	36.0	36.0	32.0	36.0
28	33.817305226255826	36.0	36.0	36.0	32.0	36.0
29	33.77353198948291	36.0	36.0	36.0	32.0	36.0
30	33.7315669541953	36.0	36.0	36.0	27.0	36.0
31	33.74588495779326	36.0	36.0	36.0	27.0	36.0
32	33.70607869366668	36.0	36.0	36.0	27.0	36.0
33	33.62349554868767	36.0	36.0	36.0	27.0	36.0
34	33.61972969232898	36.0	36.0	36.0	27.0	36.0
35	33.57231975644633	36.0	36.0	36.0	27.0	36.0
36	33.52294847548318	36.0	36.0	36.0	21.0	36.0
37	33.50302135707366	36.0	36.0	36.0	21.0	36.0
38	32.896683426357306	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	5.0
12	3.0
13	2.0
14	139.0
15	254.0
16	351.0
17	403.0
18	469.0
19	620.0
20	752.0
21	1061.0
22	1574.0
23	2255.0
24	3013.0
25	4497.0
26	6373.0
27	9109.0
28	12251.0
29	16582.0
30	21687.0
31	28491.0
32	37545.0
33	54271.0
34	114453.0
35	225809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.07994354926601	17.348441047220227	11.625969554286728	24.945645849227034
2	16.25709791991257	20.419118787243313	37.622113758703954	25.701669534140166
3	18.870831726395547	25.338379579311376	28.496035399993723	27.29475329429935
4	12.390928498545097	15.795601528523271	35.88007255134594	35.93339742158568
5	14.28885096176023	36.94487753125144	33.08067715300521	15.685594353983118
6	32.96664772349863	35.66789428236145	17.425807975319756	13.93965001882016
7	29.43715116010886	31.269892522717836	21.08455187047373	18.20840444669957
8	28.53305041745468	31.84039854236819	19.89261497301536	19.73393606716177
9	28.0924396881775	14.40675307901656	18.91101988099082	38.58978735181512
10	16.161476380785793	26.715672707546855	31.189688066217197	25.933162845450152
11	36.522703411831344	22.033075814579117	22.140462426679115	19.30375834691042
12	25.245264010539387	24.305169871966374	28.488081377648456	21.961484739845787
13	29.764956200325155	19.785421268538972	25.31043607757165	25.13918645356423
14	24.26535037136135	20.033030400885732	25.668496563177563	30.033122664575355
15	25.20065775510618	26.583899764322876	23.862262684947485	24.353179795623454
16	25.204549732249294	26.174061949874705	24.339296061056757	24.282092256819247
17	23.452563742931805	25.7762095285902	25.97275331079385	24.798473417684143
18	24.584694926294073	24.648353817965095	27.24047007939832	23.52648117634251
19	24.907368452130527	25.465187514300684	26.800594908586316	22.82684912498247
20	25.42513303859411	23.724785403765676	26.62875405020408	24.22132750743614
21	26.86077215332353	24.117105430685005	25.336799898143518	23.68532251784795
22	25.386298801707945	24.34632743967022	25.94854023892032	24.318833519701517
23	23.677489832522642	24.281818114717193	26.57826558705649	25.46242646570368
24	24.058446531351898	25.655428983736762	26.122491912669748	24.16363257224159
25	24.38552233600242	24.589625435749046	26.254375944622733	24.7704762836258
26	23.756313702187025	25.395984697634333	26.686129878182683	24.16157172199595
27	24.898740974337873	25.29049115471272	25.714718033978745	24.09604983697066
28	23.80426057994815	24.808052477649948	26.87025436160496	24.517432580796942
29	23.653665881797178	25.49251175458927	26.963034861491447	23.890787502122098
30	24.037847671977	25.443112247722166	26.851698089533954	23.66734199076688
31	24.052116085794843	25.541753391494325	25.714280442259717	24.69185008045112
32	23.513119533527696	25.769088829021662	25.723511827877626	24.994279809573015
33	23.509113790443713	25.10324287817894	26.27443599310607	25.113207338271277
34	24.078523423054353	25.484312661698976	26.515679956892573	23.92148395835409
35	25.113400235473883	25.216927545646133	26.32564782998638	23.344024388893605
36	23.852863941774483	26.185058673944855	25.702860158739828	24.259217225540837
37	24.529503051148954	26.113664755585162	25.810302863669833	23.54652932959605
38	23.872252247930135	25.846629627101002	26.145000488981314	24.136117635987546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	858.0
1	611.5
2	365.0
3	365.0
4	760.0
5	1155.0
6	1155.0
7	1303.0
8	1451.0
9	1327.5
10	1204.0
11	1204.0
12	1509.5
13	1815.0
14	2194.0
15	2573.0
16	2573.0
17	3633.0
18	4693.0
19	4693.0
20	5298.0
21	5903.0
22	5590.0
23	5277.0
24	5277.0
25	5672.5
26	6068.0
27	6068.0
28	7558.0
29	9048.0
30	10992.5
31	12937.0
32	12937.0
33	15750.0
34	18563.0
35	18563.0
36	20227.0
37	21891.0
38	25275.0
39	28659.0
40	28659.0
41	30980.0
42	33301.0
43	37763.5
44	42226.0
45	42226.0
46	45588.5
47	48951.0
48	48951.0
49	49658.0
50	50365.0
51	49341.0
52	48317.0
53	48317.0
54	45810.0
55	43303.0
56	43303.0
57	42157.5
58	41012.0
59	36517.0
60	32022.0
61	32022.0
62	30386.5
63	28751.0
64	23709.5
65	18668.0
66	18668.0
67	15507.0
68	12346.0
69	12346.0
70	9998.5
71	7651.0
72	6022.0
73	4393.0
74	4393.0
75	3290.5
76	2188.0
77	2188.0
78	2191.5
79	2195.0
80	1719.5
81	1244.0
82	1244.0
83	1247.5
84	1251.0
85	1251.0
86	846.0
87	441.0
88	369.0
89	297.0
90	297.0
91	217.0
92	137.0
93	121.5
94	106.0
95	106.0
96	92.0
97	78.0
98	78.0
99	175.0
100	272.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11402739978781308
2	0.0492642649568707
3	0.009963559204760367
4	0.002583144979011947
5	0.0
6	5.535310669311315E-4
7	0.0
8	0.0
9	0.0
10	1.845103556437105E-4
11	0.001476082845149684
12	0.001476082845149684
13	0.014022787028921999
14	0.009225517782185525
15	0.024355366944969786
16	0.009779048849116657
17	0.020480649476451868
18	0.004428248535449052
19	0.007933945292679552
20	0.0049817796023801835
21	0.006642372803173578
22	0.006826883158817289
23	0.009410028137829235
24	0.01439180774020942
25	0.016974952719221368
26	0.018082014853083632
27	0.008856497070898105
28	0.005535310669311315
29	0.01088611098297892
30	0.0023986346233682366
31	0.005719821024955026
32	0.006457862447529868
33	0.009041007426541816
34	0.013469255961990867
35	0.016421421652290234
36	0.015129849162784263
37	0.008856497070898105
38	0.005904331380598736
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	541975.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.9689089728851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.597161410391	52.42393794804989
2	10.352719533027809	12.830934687280198
3	2.3880734083760853	4.439589109926753
4	0.9032702683170397	2.2389869214100857
5	0.40988257742388695	1.2699988064976193
6	0.23793254324477936	0.8846652068413668
7	0.16322805645449168	0.7080545200574543
8	0.10470034257825751	0.5190532798929541
9	0.08145504721977585	0.4542912365889913
>10	0.5812357240481857	7.285598274339966
>50	0.09628127725324231	4.257788614018294
>100	0.07809812289485368	9.253347368140039
>500	0.004173182139419634	1.548874469634167
>1k	0.0017885066311798432	1.8848795573222468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3280	0.6051939665113705	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2228	0.411089072374187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1304	0.24060150375939848	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1291	0.23820286913603028	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1053	0.19428940449282714	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1048	0.1933668527146086	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	822	0.15166751233913003	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	731	0.13487706997555238	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	625	0.11531897227731906	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	616	0.11365837907652566	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	614	0.11328935836523825	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	589	0.1086765994741455	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	584	0.10775404769592693	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	568	0.10480188200562757	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	557	0.10277226809354675	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	557	0.10277226809354675	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	556	0.10258775773790303	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	543	0.1001891231145348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.845103556437105E-4
2	0.0	0.0	0.0	0.0	1.845103556437105E-4
3	0.0	0.0	0.0	0.0	1.845103556437105E-4
4	0.0	0.0	0.0	0.0	1.845103556437105E-4
5	0.0	0.0	0.0	0.0	1.845103556437105E-4
6	0.0	0.0	0.0	0.0	1.845103556437105E-4
7	0.0	0.0	0.0	0.0	1.845103556437105E-4
8	0.0	0.0	0.0	0.0	1.845103556437105E-4
9	0.0	0.0	0.0	0.0	1.845103556437105E-4
10	0.0	0.0	0.0	0.0	1.845103556437105E-4
11	0.0	0.0	0.0	0.0	1.845103556437105E-4
12	0.0	0.0	0.0	0.0	1.845103556437105E-4
13	0.0	0.0	0.0	0.0	1.845103556437105E-4
14	0.0	0.0	0.0	0.0	1.845103556437105E-4
15	0.0	0.0	0.0	0.0	1.845103556437105E-4
16	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
17	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
18	0.0	0.0	0.0	3.69020711287421E-4	1.845103556437105E-4
19	0.0	0.0	0.0	3.69020711287421E-4	1.845103556437105E-4
20	0.0	0.0	0.0	7.38041422574842E-4	1.845103556437105E-4
21	0.0	0.0	0.0	0.0016605932007933946	1.845103556437105E-4
22	0.0	0.0	0.0	0.002583144979011947	1.845103556437105E-4
23	0.0	0.0	0.0	0.005350800313667604	3.69020711287421E-4
24	0.0	0.0	0.0	0.008671986715254393	3.69020711287421E-4
25	0.0	0.0	0.0	0.01125513169426634	3.69020711287421E-4
26	0.0	0.0	0.0	0.014945338807140551	3.69020711287421E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATAAT	30	8.428823E-4	26.657055	6
GGCGGGT	70	3.079367E-8	22.85523	32
GTCCTAT	155	0.0	20.656778	1
CCTATAC	40	0.00448983	19.992792	3
TAGTCAG	40	0.00448983	19.992792	5
CCTATTC	180	0.0	19.548508	3
TACCGTC	100	3.254172E-9	19.198395	7
CATCGTT	125	7.2759576E-12	19.198393	28
CTATTCC	195	0.0	18.864994	4
ATCGTTT	120	8.185452E-11	18.665104	29
ATACCGT	115	9.422365E-10	18.080439	6
CCCTATA	45	0.008829867	17.782854	2
GCATTCG	225	0.0	17.777933	22
CTATACC	45	0.008862793	17.771372	4
TAAGGCT	45	0.008862793	17.771372	4
ATTCGTA	235	0.0	17.702282	24
GTAAACG	155	0.0	17.54692	27
AAACGCT	160	1.8189894E-12	16.998579	29
AAGACGG	160	1.8189894E-12	16.993874	5
TCTTTCG	180	0.0	16.890594	10
>>END_MODULE