FastQCFastQC Report
Thu 2 Feb 2017
SRR4062894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541975
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38970.7190368559435398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27620.5096176022879284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16780.30960837677014624No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13690.2525946768762397No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12720.23469717237879975No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.22934637206513214No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7060.1302643110844596No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC6900.12731214539416025No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG6760.12472900041514831No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA6640.12251487614742378No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT6520.12030075187969924No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG6220.11476544121038794No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA6190.1142119101434568No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC6110.11273582729830713No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC5960.10996817196365145No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.10904562018543291No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5620.1036948198717653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5590.10314128880483417No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5560.10258775773790303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT200.003751939631.9997226
CTAATAG350.002065244122.8569453
AACCCGT604.7133162E-621.33314929
TAACCCG604.7133162E-621.33314928
TTAGGCA555.8263966E-520.363464
TCCTATA400.004480700519.9998262
ACCGTCG1003.252353E-919.1998338
CGTCGTA1105.18412E-1018.90892810
CCGTCGT1055.9571903E-918.2855579
CCCGTTG701.7845578E-518.28555731
CGCAAGA1600.017.9998442
ACATAAC450.00883075417.7825471
CTAGTAG450.0088448517.7776263
GAACCGC904.356607E-717.7776266
GTAGTTA450.0088448517.7776266
TAGGCAT550.001363464217.4543935
TATATAG652.0979636E-417.230625
ACCCGTT753.2288386E-517.06651930
CCGTTGA753.2288386E-517.06651932
TTTTCGG1601.8189894E-1216.99985129
Produced by FastQC (version 0.11.5)