##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541975
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081411504220675	32.0	32.0	32.0	32.0	32.0
2	31.269161861709488	32.0	32.0	32.0	32.0	32.0
3	31.34782969694174	32.0	32.0	32.0	32.0	32.0
4	31.455829143410675	32.0	32.0	32.0	32.0	32.0
5	31.379783200332117	32.0	32.0	32.0	32.0	32.0
6	34.88274920429909	36.0	36.0	36.0	36.0	36.0
7	34.89984224364593	36.0	36.0	36.0	36.0	36.0
8	34.858914156557034	36.0	36.0	36.0	32.0	36.0
9	34.951785598966744	36.0	36.0	36.0	32.0	36.0
10	34.792339130033675	36.0	36.0	36.0	32.0	36.0
11	34.97734581853407	36.0	36.0	36.0	36.0	36.0
12	34.86465611882467	36.0	36.0	36.0	32.0	36.0
13	34.91224318464874	36.0	36.0	36.0	32.0	36.0
14	34.867921952119566	36.0	36.0	36.0	32.0	36.0
15	34.82444208681212	36.0	36.0	36.0	32.0	36.0
16	34.83306794593847	36.0	36.0	36.0	32.0	36.0
17	34.79980626412657	36.0	36.0	36.0	32.0	36.0
18	34.79137967618433	36.0	36.0	36.0	32.0	36.0
19	34.77717053369621	36.0	36.0	36.0	32.0	36.0
20	34.75935236865169	36.0	36.0	36.0	32.0	36.0
21	34.739214908436736	36.0	36.0	36.0	32.0	36.0
22	34.72460353337331	36.0	36.0	36.0	32.0	36.0
23	34.67721942894045	36.0	36.0	36.0	32.0	36.0
24	34.65164260344112	36.0	36.0	36.0	32.0	36.0
25	34.610908252225656	36.0	36.0	36.0	32.0	36.0
26	34.542783338714884	36.0	36.0	36.0	32.0	36.0
27	34.53240647631348	36.0	36.0	36.0	32.0	36.0
28	34.488491166566725	36.0	36.0	36.0	32.0	36.0
29	34.45210756953734	36.0	36.0	36.0	32.0	36.0
30	34.42735735043129	36.0	36.0	36.0	32.0	36.0
31	34.410266156188015	36.0	36.0	36.0	32.0	36.0
32	34.35679136491535	36.0	36.0	36.0	32.0	36.0
33	34.29325891415656	36.0	36.0	36.0	32.0	36.0
34	34.26151759767517	36.0	36.0	36.0	32.0	36.0
35	34.220753724802805	36.0	36.0	36.0	32.0	36.0
36	34.16967941325707	36.0	36.0	36.0	32.0	36.0
37	34.1550994049541	36.0	36.0	36.0	32.0	36.0
38	33.72108676599474	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	8.0
21	11.0
22	49.0
23	143.0
24	425.0
25	969.0
26	2139.0
27	4013.0
28	7241.0
29	11391.0
30	16879.0
31	24134.0
32	33919.0
33	52371.0
34	121666.0
35	266615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52871707339577	18.462702403469113	12.35595331457305	25.652627208562066
2	15.405507634115963	21.58383689284561	36.75464735458277	26.256008118455647
3	17.903962359887448	25.972415701831263	28.854098436274732	27.26952350200655
4	12.012015336529059	16.560757527113847	35.789539719617544	35.63768741673955
5	13.732393066826084	37.48260986689399	32.935897293966136	15.849099772313801
6	33.106569687844804	36.033093838449815	16.989006852727254	13.871329620978129
7	29.06602358420069	31.431824094558213	21.193122166602397	18.3090301546387
8	28.366330239681165	32.09550344114988	19.8614314445449	19.676734874624056
9	27.889893698484542	14.223478593004138	18.80712022718005	39.07950748133127
10	16.00340977024643	26.938845549216566	31.231687245835577	25.826057434701422
11	36.827703026858664	21.775520898684007	22.069091499553462	19.327684574903863
12	24.98763773774291	24.533924261769982	28.469920955326106	22.008517045161007
13	29.810046588864804	19.79759213985885	25.458554361363532	24.93380690991282
14	24.066054707320447	19.964020480649477	25.68568660916094	30.284238202869133
15	25.258729646201395	26.574472992296695	23.757737903039807	24.409059458462107
16	25.21721481618156	25.931823423589652	24.36754462844227	24.483417131786524
17	23.510863047188522	25.802112643572123	25.94769131417501	24.73933299506435
18	24.698925227178375	24.264956870704367	27.571197933484015	23.46491996863324
19	25.00502790719129	25.3891784676415	26.889801190091795	22.715992435075417
20	25.567046450482035	23.44056460168827	26.839798883712344	24.152590064117348
21	26.766413148970248	24.065545579677252	25.60251967806574	23.56552159328676
22	25.36044164480215	24.275703262518128	25.92246122507722	24.441393867602503
23	23.659437537594055	23.978272020429024	26.916973876975646	25.445316565001274
24	23.98505466119286	25.489921121822963	26.369297476820886	24.155726740163292
25	24.523271368605563	24.210526315789473	26.42077586604548	24.845426449559483
26	23.91807740209419	25.105770561372758	27.00493565201347	23.971216384519582
27	24.895428755938926	24.96609622215047	26.088841736242447	24.049633285668158
28	23.83246459707551	24.486000276765534	27.18040500023064	24.501130125928317
29	23.664006642372804	25.074034780202037	27.24166243830435	24.02029613912081
30	23.922321140273997	24.99801651367683	27.399972323446654	23.679690022602518
31	24.11993173116841	25.047465288989347	26.06264126574104	24.769961714101203
32	23.57248950597352	25.267770653627935	26.084228977351355	25.07551086304719
33	23.346279809954336	24.77033073481249	26.78112459061765	25.102264864615524
34	24.14779279487061	24.971078001752847	26.935744268647078	23.94538493472946
35	25.0869505050971	24.769592693389917	26.643664375663086	23.4997924258499
36	23.93136214770054	25.760597813552288	26.066146962498273	24.241893076248903
37	24.805756723096085	25.63845195811615	26.054338299737072	23.501453019050693
38	23.893906545504866	25.186217076433415	26.582037916878086	24.337838461183633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	456.0
1	341.0
2	226.0
3	226.0
4	639.0
5	1052.0
6	1052.0
7	1219.5
8	1387.0
9	1353.0
10	1319.0
11	1319.0
12	1610.5
13	1902.0
14	2303.0
15	2704.0
16	2704.0
17	3951.5
18	5199.0
19	5199.0
20	5790.0
21	6381.0
22	5968.5
23	5556.0
24	5556.0
25	5633.5
26	5711.0
27	5711.0
28	7301.5
29	8892.0
30	10711.0
31	12530.0
32	12530.0
33	15541.0
34	18552.0
35	18552.0
36	20200.0
37	21848.0
38	25022.5
39	28197.0
40	28197.0
41	30664.5
42	33132.0
43	37699.0
44	42266.0
45	42266.0
46	45753.0
47	49240.0
48	49240.0
49	50622.0
50	52004.0
51	50440.5
52	48877.0
53	48877.0
54	45995.5
55	43114.0
56	43114.0
57	41965.0
58	40816.0
59	36554.0
60	32292.0
61	32292.0
62	30392.0
63	28492.0
64	23487.5
65	18483.0
66	18483.0
67	15434.0
68	12385.0
69	12385.0
70	10007.0
71	7629.0
72	5993.5
73	4358.0
74	4358.0
75	3254.5
76	2151.0
77	2151.0
78	2146.0
79	2141.0
80	1617.0
81	1093.0
82	1093.0
83	1079.5
84	1066.0
85	1066.0
86	671.0
87	276.0
88	234.5
89	193.0
90	193.0
91	112.0
92	31.0
93	21.5
94	12.0
95	12.0
96	8.5
97	5.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009225517782185525
2	0.0
3	0.0
4	1.845103556437105E-4
5	1.845103556437105E-4
6	1.845103556437105E-4
7	3.69020711287421E-4
8	9.225517782185526E-4
9	0.004797269246736473
10	5.535310669311315E-4
11	0.0049817796023801835
12	5.535310669311315E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.845103556437105E-4
22	1.845103556437105E-4
23	1.845103556437105E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	541975.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.84940065771428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.53641528014998	49.996043876109006
2	10.98690482795399	13.151193380727873
3	2.623495633827302	4.710439239380828
4	0.9530596705707035	2.2816020029878086
5	0.4473895189467634	1.3387997284753446
6	0.2758221746011132	0.9904675102790427
7	0.15672381956715203	0.6565878668917262
8	0.11615668821301811	0.5561526537547294
9	0.10108322520857582	0.5444793400753817
>10	0.6051664907548508	7.259044419826058
>50	0.10321783209258685	4.377904944585563
>100	0.08776605890320383	10.094335201465977
>500	0.004944566698772037	1.7826044427456433
>1k	0.0018542125120395137	2.2603453926950103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3897	0.7190368559435398	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2762	0.5096176022879284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1678	0.30960837677014624	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1369	0.2525946768762397	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1272	0.23469717237879975	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1243	0.22934637206513214	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	706	0.1302643110844596	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	690	0.12731214539416025	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	676	0.12472900041514831	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	664	0.12251487614742378	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	652	0.12030075187969924	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	622	0.11476544121038794	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	619	0.1142119101434568	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	611	0.11273582729830713	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	596	0.10996817196365145	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	591	0.10904562018543291	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	562	0.1036948198717653	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	559	0.10314128880483417	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	556	0.10258775773790303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.845103556437105E-4
5	0.0	0.0	0.0	0.0	1.845103556437105E-4
6	0.0	0.0	0.0	0.0	1.845103556437105E-4
7	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
8	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
9	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
10	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
11	0.0	0.0	0.0	1.845103556437105E-4	1.845103556437105E-4
12	0.0	0.0	0.0	1.845103556437105E-4	3.69020711287421E-4
13	0.0	0.0	0.0	1.845103556437105E-4	3.69020711287421E-4
14	0.0	0.0	0.0	1.845103556437105E-4	3.69020711287421E-4
15	0.0	0.0	0.0	1.845103556437105E-4	3.69020711287421E-4
16	0.0	0.0	0.0	3.69020711287421E-4	3.69020711287421E-4
17	0.0	0.0	0.0	3.69020711287421E-4	3.69020711287421E-4
18	0.0	0.0	0.0	5.535310669311315E-4	3.69020711287421E-4
19	0.0	0.0	0.0	5.535310669311315E-4	3.69020711287421E-4
20	0.0	0.0	0.0	9.225517782185525E-4	3.69020711287421E-4
21	0.0	0.0	0.0	0.001845103556437105	3.69020711287421E-4
22	0.0	0.0	0.0	0.002583144979011947	5.535310669311315E-4
23	0.0	0.0	0.0	0.005166289958023894	5.535310669311315E-4
24	0.0	0.0	0.0	0.008487476359610684	5.535310669311315E-4
25	0.0	0.0	0.0	0.011070621338622631	5.535310669311315E-4
26	0.0	0.0	0.0	0.01439180774020942	5.535310669311315E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCGT	20	0.0037519396	31.999722	6
CTAATAG	35	0.0020652441	22.856945	3
AACCCGT	60	4.7133162E-6	21.333149	29
TAACCCG	60	4.7133162E-6	21.333149	28
TTAGGCA	55	5.8263966E-5	20.36346	4
TCCTATA	40	0.0044807005	19.999826	2
ACCGTCG	100	3.252353E-9	19.199833	8
CGTCGTA	110	5.18412E-10	18.908928	10
CCGTCGT	105	5.9571903E-9	18.285557	9
CCCGTTG	70	1.7845578E-5	18.285557	31
CGCAAGA	160	0.0	17.999844	2
ACATAAC	45	0.008830754	17.782547	1
CTAGTAG	45	0.00884485	17.777626	3
GAACCGC	90	4.356607E-7	17.777626	6
GTAGTTA	45	0.00884485	17.777626	6
TAGGCAT	55	0.0013634642	17.454393	5
TATATAG	65	2.0979636E-4	17.23062	5
ACCCGTT	75	3.2288386E-5	17.066519	30
CCGTTGA	75	3.2288386E-5	17.066519	32
TTTTCGG	160	1.8189894E-12	16.999851	29
>>END_MODULE