FastQCFastQC Report
Thu 2 Feb 2017
SRR4062893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441059
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33880.23510487773227884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21390.14843250692719728No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18220.12643479552190437No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18030.12511632070581424No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14820.1028410356550287No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14500.10062044649108745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG400.004487992319.996943
TCTAGCG3150.017.77690928
CTAGCGG3300.016.96945829
ATCGTTT2550.016.31349629
CTATTCC4150.015.8037154
CGTCGTA3450.015.76789510
TAGCGGC3700.015.56734830
GTCCTAT4050.015.4259271
TCTAGTA859.416438E-515.0622712
GTATTAG3200.015.018031
ACCGTCG3850.014.960318
CGAACGA2150.014.88299516
TAGAGTG3350.014.8026785
CCTATTC4250.014.6801083
CATCGTT2950.014.64335328
GCATCGT2850.014.59577927
CCGTCGT3950.014.5820739
ATACCGT3950.014.5795436
TAACGAA2200.014.54524913
ACGAACG2200.014.54524915
Produced by FastQC (version 0.11.5)