##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062893_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1441059
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22767770091301	32.0	32.0	32.0	32.0	32.0
2	30.81682221199826	32.0	32.0	32.0	32.0	32.0
3	30.861546265628263	32.0	32.0	32.0	32.0	32.0
4	30.939217617044132	32.0	32.0	32.0	32.0	32.0
5	30.831231753869897	32.0	32.0	32.0	32.0	32.0
6	34.49163705302836	36.0	36.0	36.0	32.0	36.0
7	34.426648041475055	36.0	36.0	36.0	32.0	36.0
8	34.41046757974517	36.0	36.0	36.0	32.0	36.0
9	34.558259585485395	36.0	36.0	36.0	32.0	36.0
10	34.256943678225525	36.0	36.0	36.0	32.0	36.0
11	34.540177050349776	36.0	36.0	36.0	32.0	36.0
12	34.35213894781546	36.0	36.0	36.0	32.0	36.0
13	34.43650745736295	36.0	36.0	36.0	32.0	36.0
14	34.33049583674229	36.0	36.0	36.0	32.0	36.0
15	34.27730717479298	36.0	36.0	36.0	32.0	36.0
16	34.28835807555416	36.0	36.0	36.0	32.0	36.0
17	34.21300793374872	36.0	36.0	36.0	32.0	36.0
18	34.242425188698036	36.0	36.0	36.0	32.0	36.0
19	34.22941808767025	36.0	36.0	36.0	32.0	36.0
20	34.20938906734561	36.0	36.0	36.0	32.0	36.0
21	34.19433347281409	36.0	36.0	36.0	32.0	36.0
22	34.174509163053	36.0	36.0	36.0	32.0	36.0
23	34.108859526223426	36.0	36.0	36.0	32.0	36.0
24	34.09898553771914	36.0	36.0	36.0	32.0	36.0
25	34.07639520658071	36.0	36.0	36.0	32.0	36.0
26	34.03215968256678	36.0	36.0	36.0	32.0	36.0
27	34.02471238165821	36.0	36.0	36.0	32.0	36.0
28	34.011236875103656	36.0	36.0	36.0	32.0	36.0
29	33.96898947232556	36.0	36.0	36.0	32.0	36.0
30	33.94794453245842	36.0	36.0	36.0	32.0	36.0
31	33.96874520751753	36.0	36.0	36.0	32.0	36.0
32	33.91630391260871	36.0	36.0	36.0	32.0	36.0
33	33.875683091393206	36.0	36.0	36.0	32.0	36.0
34	33.88435796174896	36.0	36.0	36.0	32.0	36.0
35	33.84192458462839	36.0	36.0	36.0	32.0	36.0
36	33.803955285661445	36.0	36.0	36.0	32.0	36.0
37	33.799571009930894	36.0	36.0	36.0	32.0	36.0
38	33.20543919437025	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	4.0
9	10.0
10	9.0
11	9.0
12	10.0
13	7.0
14	160.0
15	359.0
16	502.0
17	601.0
18	794.0
19	1028.0
20	1324.0
21	1814.0
22	2800.0
23	4217.0
24	6553.0
25	9766.0
26	14327.0
27	20729.0
28	29269.0
29	39732.0
30	52857.0
31	70823.0
32	95900.0
33	142984.0
34	312358.0
35	632108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92323581541189	16.96446375333411	11.28583448378403	25.826465947469966
2	16.91767510937284	19.200392707690366	37.13566398219207	26.746268200744726
3	19.47656987396591	23.025373382932653	28.05035811448559	29.447698628615846
4	12.882534716897071	14.836993143018141	35.55991514302785	36.72055699705693
5	15.024259987120555	35.99069016054893	33.43235793751249	15.552691914818023
6	35.129956871875116	34.77313496015073	16.435805711787545	13.661102456186613
7	30.903314853867887	30.02208792284008	20.37196256364243	18.702634659649604
8	28.527928574353023	32.582330535144145	19.211880773130165	19.677860117372667
9	27.322432053205503	14.478222190146933	18.403127155540318	39.79621860110725
10	16.199646091391692	26.27688144061622	31.18476111168939	26.338711356302696
11	37.860177401379296	21.14430967935346	21.672266843207545	19.323246076059696
12	25.098315708098635	23.427625274024457	28.260448667993042	23.21361034988387
13	29.562723128772024	18.990444675168924	25.19266111077181	26.254171085287243
14	23.776083035434826	19.475865189561997	24.811249980047567	31.93680179495561
15	25.460576408520847	26.64745906469482	22.32908022146814	25.562884305316192
16	26.18826362598506	25.64284251911125	23.261908314566192	24.906985540337494
17	24.422971612042584	25.68443496505729	24.86377236885119	25.028821054048933
18	25.31638278500128	24.563004519159236	25.867398207881326	24.25321448795816
19	25.667146211078656	25.0155626388406	25.167545600093273	24.149745549987472
20	25.768677193747042	24.152557075660905	25.02196418831047	25.056801542281583
21	26.835459904203685	24.155258873511944	24.401203630322268	24.608077591962104
22	25.765097042876246	24.099403106676725	25.00019084485978	25.135309005587242
23	24.47103973794884	23.985037544935945	25.4797562702125	26.064166446902714
24	24.89138521672987	25.053856275921802	24.968699509741324	25.086058997607
25	25.130375155638163	24.33070385233079	25.06541314037209	25.47350785165896
26	24.741292075174172	25.207278663311154	25.539729625059167	24.511699636455504
27	25.568240176551416	24.597137979374576	24.715602315155387	25.119019528918624
28	24.80579108297582	24.44846333105247	25.546401253596525	25.199344332375183
29	24.568904792275507	25.00543054694803	25.537173048108748	24.888491612667714
30	24.827829331053454	24.948300222343743	25.61248622495857	24.611384221644233
31	25.106212881009647	24.75922535729305	24.65152042993142	25.483041331765875
32	24.87975055570476	24.66683738583156	24.734847459650247	25.71856459881343
33	24.467845900164892	24.47395304163405	25.322221111832867	25.735979946368193
34	25.39723960549136	24.551298823428166	25.31576167636328	24.73569989471719
35	25.820556170932196	24.380158406276287	25.35062587968785	24.448659543103663
36	24.647728479390477	25.173385672336646	24.857672103454664	25.32121374481821
37	25.703193640756012	24.861026867444963	24.802106423230615	24.633673068568406
38	24.75579051975883	24.68514395798403	25.218393644307746	25.34067187794939
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	117.0
1	168.0
2	219.0
3	219.0
4	488.5
5	758.0
6	758.0
7	924.5
8	1091.0
9	1029.5
10	968.0
11	968.0
12	1334.0
13	1700.0
14	2361.5
15	3023.0
16	3023.0
17	4484.5
18	5946.0
19	5946.0
20	7341.0
21	8736.0
22	9556.0
23	10376.0
24	10376.0
25	12358.0
26	14340.0
27	14340.0
28	18023.0
29	21706.0
30	26021.5
31	30337.0
32	30337.0
33	38185.0
34	46033.0
35	46033.0
36	51560.0
37	57087.0
38	65479.5
39	73872.0
40	73872.0
41	80203.0
42	86534.0
43	97916.5
44	109299.0
45	109299.0
46	114963.5
47	120628.0
48	120628.0
49	127149.0
50	133670.0
51	135628.5
52	137587.0
53	137587.0
54	132975.5
55	128364.0
56	128364.0
57	124033.5
58	119703.0
59	108177.0
60	96651.0
61	96651.0
62	89992.0
63	83333.0
64	69430.0
65	55527.0
66	55527.0
67	46248.5
68	36970.0
69	36970.0
70	29465.5
71	21961.0
72	17141.0
73	12321.0
74	12321.0
75	9447.0
76	6573.0
77	6573.0
78	6120.0
79	5667.0
80	4503.5
81	3340.0
82	3340.0
83	3241.0
84	3142.0
85	3142.0
86	2161.5
87	1181.0
88	942.5
89	704.0
90	704.0
91	541.0
92	378.0
93	319.0
94	260.0
95	260.0
96	249.0
97	238.0
98	238.0
99	478.5
100	719.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12268755130775354
2	0.05565351592127734
3	0.012421420635796314
4	0.003192096923165533
5	2.081802341194913E-4
6	2.775736454926551E-4
7	0.0
8	5.551472909853102E-4
9	3.469670568658188E-4
10	6.245407023584738E-4
11	0.0022899825753144045
12	0.002636949632180223
13	0.012976567926781623
14	0.009159930301257618
15	0.021511957525680764
16	0.01068658535146722
17	0.018875007893500544
18	0.0057596531439725925
19	0.007494488428301686
20	0.00534329267573361
21	0.00589843996671892
22	0.006800554314570049
23	0.008257815953406487
24	0.012976567926781623
25	0.015058370267976537
26	0.017001385786425124
27	0.008257815953406487
28	0.00589843996671892
29	0.009437503946750272
30	0.003261490334538697
31	0.00589843996671892
32	0.006661767491823721
33	0.008535389598899143
34	0.012352027224423152
35	0.014364436154244898
36	0.013601108629140098
37	0.009229323712630782
38	0.0057596531439725925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1441059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.4040197525342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.61579972817641	47.66690779443291
2	11.346702133060552	13.25386031010781
3	3.1150534248424493	5.4579492526409314
4	1.3180087238174019	3.0790803015937573
5	0.7044619736084765	2.0571705510769345
6	0.4212039841228406	1.476000348513389
7	0.2847688384883491	1.1642151407585832
8	0.18451340771809588	0.8621059767180054
9	0.1435292176657935	0.7544414937256906
>10	0.7195312866200803	7.504014213052455
>50	0.06662448167356438	2.6968897238159295
>100	0.06894777633334503	8.497482844006004
>500	0.007753588480478927	3.0309675395029716
>1k	0.0031014353921915714	2.498914510054523
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3388	0.23510487773227884	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2139	0.14843250692719728	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1822	0.12643479552190437	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1803	0.12511632070581424	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1482	0.1028410356550287	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1450	0.10062044649108745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.939341137316377E-5
7	0.0	0.0	0.0	1.3878682274632754E-4	6.939341137316377E-5
8	0.0	0.0	0.0	1.3878682274632754E-4	6.939341137316377E-5
9	0.0	0.0	0.0	1.3878682274632754E-4	6.939341137316377E-5
10	0.0	0.0	0.0	1.3878682274632754E-4	6.939341137316377E-5
11	0.0	0.0	0.0	1.3878682274632754E-4	6.939341137316377E-5
12	0.0	0.0	0.0	1.3878682274632754E-4	1.3878682274632754E-4
13	0.0	0.0	0.0	1.3878682274632754E-4	2.081802341194913E-4
14	0.0	0.0	0.0	1.3878682274632754E-4	2.081802341194913E-4
15	0.0	0.0	0.0	1.3878682274632754E-4	2.081802341194913E-4
16	0.0	0.0	0.0	2.081802341194913E-4	2.081802341194913E-4
17	0.0	0.0	0.0	2.775736454926551E-4	2.081802341194913E-4
18	0.0	0.0	0.0	3.469670568658188E-4	2.081802341194913E-4
19	0.0	0.0	0.0	4.163604682389826E-4	2.081802341194913E-4
20	0.0	0.0	0.0	4.8575387961214637E-4	2.775736454926551E-4
21	0.0	0.0	0.0	6.245407023584738E-4	2.775736454926551E-4
22	0.0	0.0	0.0	9.02114347851129E-4	3.469670568658188E-4
23	0.0	0.0	0.0	0.0011102945819706203	4.163604682389826E-4
24	0.0	0.0	0.0	0.0015266550502096028	4.163604682389826E-4
25	0.0	0.0	0.0	0.002081802341194913	4.163604682389826E-4
26	0.0	0.0	0.0	0.0027757364549265504	4.8575387961214637E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATCG	40	0.0044879923	19.99694	3
TCTAGCG	315	0.0	17.776909	28
CTAGCGG	330	0.0	16.969458	29
ATCGTTT	255	0.0	16.313496	29
CTATTCC	415	0.0	15.803715	4
CGTCGTA	345	0.0	15.767895	10
TAGCGGC	370	0.0	15.567348	30
GTCCTAT	405	0.0	15.425927	1
TCTAGTA	85	9.416438E-5	15.062271	2
GTATTAG	320	0.0	15.01803	1
ACCGTCG	385	0.0	14.96031	8
CGAACGA	215	0.0	14.882995	16
TAGAGTG	335	0.0	14.802678	5
CCTATTC	425	0.0	14.680108	3
CATCGTT	295	0.0	14.643353	28
GCATCGT	285	0.0	14.595779	27
CCGTCGT	395	0.0	14.582073	9
ATACCGT	395	0.0	14.579543	6
TAACGAA	220	0.0	14.545249	13
ACGAACG	220	0.0	14.545249	15
>>END_MODULE