FastQCFastQC Report
Thu 2 Feb 2017
SRR4062893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441059
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37060.2571719825489449No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24530.17022203809837072No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19750.13705198746199843No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18460.12810023739486032No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15620.1083925085648818No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15530.10776796786252332No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15380.10672706669192587No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14860.10311860930052134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14780.10256346200953603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT400.004482362420.0012811
ACGATTA400.004485063719.99919732
ATCGTTT2400.018.66591829
TCTAGCG3300.018.42350428
CTAGCGG3350.018.14852729
CATCGTT2600.017.84543828
CGTCGTA2800.017.7154210
TCGCGTA1453.6379788E-1217.6575289
CGTCTTA1201.6607373E-917.33263815
GTATTAG3700.016.4329131
AACCGCG2250.016.3577387
TACCGTC3150.016.2561367
GCGCAAG4350.016.1843811
ACCGTCG3100.016.0021348
CGCGTAA1601.8189894E-1116.00102410
GTCTAGG1601.8189894E-1116.000471
CAAGACG4500.016.000474
TAGCGGC3800.015.99935730
TAGCGTA3350.015.7632987
CGCAAGA4700.015.3185352
Produced by FastQC (version 0.11.5)