##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1441059
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2018737608939	32.0	32.0	32.0	32.0	32.0
2	31.25270651652708	32.0	32.0	32.0	32.0	32.0
3	31.35618527763263	32.0	32.0	32.0	32.0	32.0
4	31.457582930331096	32.0	32.0	32.0	32.0	32.0
5	31.371113882221337	32.0	32.0	32.0	32.0	32.0
6	34.90701074695762	36.0	36.0	36.0	36.0	36.0
7	34.9275727086816	36.0	36.0	36.0	36.0	36.0
8	34.865326124745756	36.0	36.0	36.0	32.0	36.0
9	34.989036534937156	36.0	36.0	36.0	36.0	36.0
10	34.815398953130995	36.0	36.0	36.0	32.0	36.0
11	34.99988550087124	36.0	36.0	36.0	36.0	36.0
12	34.899979112583175	36.0	36.0	36.0	32.0	36.0
13	34.95561389228338	36.0	36.0	36.0	36.0	36.0
14	34.89697437787072	36.0	36.0	36.0	32.0	36.0
15	34.86427620243168	36.0	36.0	36.0	32.0	36.0
16	34.87315855908745	36.0	36.0	36.0	32.0	36.0
17	34.838891398617264	36.0	36.0	36.0	32.0	36.0
18	34.84453030722545	36.0	36.0	36.0	32.0	36.0
19	34.835261429268336	36.0	36.0	36.0	32.0	36.0
20	34.8244603447881	36.0	36.0	36.0	32.0	36.0
21	34.81466199510221	36.0	36.0	36.0	32.0	36.0
22	34.7962866197706	36.0	36.0	36.0	32.0	36.0
23	34.74228119736944	36.0	36.0	36.0	32.0	36.0
24	34.71979773208453	36.0	36.0	36.0	32.0	36.0
25	34.69795407405248	36.0	36.0	36.0	32.0	36.0
26	34.63095751110815	36.0	36.0	36.0	32.0	36.0
27	34.60665802024761	36.0	36.0	36.0	32.0	36.0
28	34.57409793769721	36.0	36.0	36.0	32.0	36.0
29	34.54368211155823	36.0	36.0	36.0	32.0	36.0
30	34.52078853121211	36.0	36.0	36.0	32.0	36.0
31	34.51806137014515	36.0	36.0	36.0	32.0	36.0
32	34.48595720230747	36.0	36.0	36.0	32.0	36.0
33	34.4543235217989	36.0	36.0	36.0	32.0	36.0
34	34.452509578025605	36.0	36.0	36.0	32.0	36.0
35	34.407920147613666	36.0	36.0	36.0	32.0	36.0
36	34.37604012049472	36.0	36.0	36.0	32.0	36.0
37	34.37330255041605	36.0	36.0	36.0	32.0	36.0
38	33.93755217517118	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	23.0
22	103.0
23	323.0
24	890.0
25	2349.0
26	4879.0
27	9671.0
28	16949.0
29	26719.0
30	40831.0
31	59351.0
32	85837.0
33	136792.0
34	331165.0
35	725168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18363517249803	18.1682980046414	12.007822649596376	26.640244173264193
2	15.975196019038776	20.177661011797575	36.459506515694365	27.387636453469288
3	18.440258171247674	23.79555590714884	28.322157524431685	29.442028397171804
4	12.388883722931347	15.683893361682873	35.56192741721707	36.365295498168706
5	14.476377772442191	36.62468824988307	33.063693480762055	15.835240496912684
6	35.14184379937782	35.23143081779555	16.115878830608366	13.510846552218267
7	30.7334022182421	30.23376688264329	20.2087226614464	18.824108237668202
8	28.326525740473187	32.90850067798351	18.97382303589496	19.791150545648346
9	27.22088210719748	14.279349675642816	18.188915506133224	40.31085271102648
10	15.966885142379313	26.515688267888926	31.191878116228157	26.325548473503602
11	38.11461957136502	21.05860333811476	21.509195395119892	19.317581695400325
12	24.891659085404907	23.53563678782113	28.16627840891348	23.406425717860483
13	29.519692169972693	19.034734968477956	25.19091915298167	26.254653708567684
14	23.621864198481813	19.43640059150944	24.729591224231623	32.212143985777125
15	25.393616777661425	26.70792798906915	22.146074518808735	25.75238071446069
16	26.170998093760346	25.72160573801036	23.16133227207529	24.946063896154
17	24.41843116763436	25.780554439478188	24.743886266974496	25.057128125912957
18	25.395976153648114	24.49129424957618	25.83058708907824	24.28214250769746
19	25.625182591413676	24.998976447182244	25.1273542582226	24.24848670318148
20	25.818165668442443	24.11122653548536	24.97538268731537	25.095225108756825
21	26.76378968522455	24.25570361796429	24.352646213652598	24.627860483158567
22	25.71234071609837	24.20289523190931	24.91577374694582	25.168990305046496
23	24.376967739652216	23.98156353287899	25.4796305767225	26.16183815074629
24	24.935342688953057	25.03325679240059	24.88260369630945	25.148796822336905
25	25.14437309254728	24.301103772367245	25.082265946270034	25.47225718881544
26	24.792600441758456	25.08786940715127	25.433032235321384	24.68649791576889
27	25.53679310617616	24.63481691923573	24.65903523661088	25.169354737977233
28	24.77518269550379	24.38713473910506	25.58403229846939	25.253650266921756
29	24.659365091921984	24.790379852594516	25.544755627632178	25.005499427851323
30	24.877884944336078	24.773725433864957	25.673202832083902	24.675186789715063
31	25.177109334177157	24.7040544488463	24.682681278143363	25.436154938833177
32	24.9543564836693	24.72334581720804	24.59968675814106	25.7226109409816
33	24.476305272719575	24.34501293840155	25.35017650214183	25.828505286737048
34	25.3181861394988	24.515373763322668	25.42352533796326	24.74291475921527
35	25.891583897675254	24.357087391980482	25.232762850098432	24.518565860245833
36	24.749229559650228	25.03991855989241	24.95088681310064	25.259965067356717
37	25.75862612148427	24.814459366341005	24.72993819128849	24.69697632088624
38	24.8289798189941	24.45141000570414	25.241662722805053	25.477947452496707
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	67.0
2	88.0
3	88.0
4	331.0
5	574.0
6	574.0
7	782.0
8	990.0
9	1037.5
10	1085.0
11	1085.0
12	1378.5
13	1672.0
14	2350.0
15	3028.0
16	3028.0
17	4462.5
18	5897.0
19	5897.0
20	7299.0
21	8701.0
22	9579.0
23	10457.0
24	10457.0
25	12367.0
26	14277.0
27	14277.0
28	18023.5
29	21770.0
30	26022.5
31	30275.0
32	30275.0
33	38331.5
34	46388.0
35	46388.0
36	51989.0
37	57590.0
38	65827.0
39	74064.0
40	74064.0
41	79839.0
42	85614.0
43	97124.5
44	108635.0
45	108635.0
46	115095.0
47	121555.0
48	121555.0
49	128589.5
50	135624.0
51	136450.0
52	137276.0
53	137276.0
54	132831.0
55	128386.0
56	128386.0
57	124063.5
58	119741.0
59	108498.5
60	97256.0
61	97256.0
62	90408.0
63	83560.0
64	69376.0
65	55192.0
66	55192.0
67	46289.5
68	37387.0
69	37387.0
70	29805.0
71	22223.0
72	17252.5
73	12282.0
74	12282.0
75	9313.5
76	6345.0
77	6345.0
78	6090.0
79	5835.0
80	4521.5
81	3208.0
82	3208.0
83	2942.0
84	2676.0
85	2676.0
86	1707.5
87	739.0
88	595.0
89	451.0
90	451.0
91	275.0
92	99.0
93	68.5
94	38.0
95	38.0
96	30.0
97	22.0
98	22.0
99	17.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007980242307913832
2	0.0
3	0.0
4	6.939341137316377E-5
5	6.939341137316377E-5
6	1.3878682274632754E-4
7	5.551472909853102E-4
8	0.001457261638836439
9	0.003816637625524007
10	9.715077592242926E-4
11	0.004579965150628809
12	4.163604682389826E-4
13	2.775736454926551E-4
14	0.0
15	0.0
16	1.3878682274632754E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.3878682274632754E-4
24	0.0
25	6.939341137316377E-5
26	0.0
27	6.939341137316377E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.939341137316377E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1441059.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.37872204591762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1817888656829	45.20546787602788
2	12.199244370206065	13.755556150361462
3	3.4281100641138726	5.798173933424666
4	1.4070093412631257	3.1730155426833364
5	0.7155622958420957	2.017124389191009
6	0.44630347851864266	1.509721185811723
7	0.32208295808138765	1.2711037898578241
8	0.20914727475155945	0.9433164855903469
9	0.15320270813449874	0.7773635608737072
>10	0.7809287613545557	7.834330169648752
>50	0.07053790351444338	2.7777865776209363
>100	0.07433234695011962	8.927133868276602
>500	0.00853394294191665	3.3431756457835644
>1k	0.003215688644780187	2.6667308248481936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3706	0.2571719825489449	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2453	0.17022203809837072	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1975	0.13705198746199843	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1846	0.12810023739486032	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1562	0.1083925085648818	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1553	0.10776796786252332	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1538	0.10672706669192587	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1486	0.10311860930052134	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1478	0.10256346200953603	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.939341137316377E-5
2	0.0	0.0	0.0	0.0	6.939341137316377E-5
3	0.0	0.0	0.0	0.0	6.939341137316377E-5
4	0.0	0.0	0.0	0.0	6.939341137316377E-5
5	0.0	0.0	0.0	0.0	6.939341137316377E-5
6	0.0	0.0	0.0	0.0	6.939341137316377E-5
7	0.0	0.0	0.0	0.0	6.939341137316377E-5
8	0.0	0.0	0.0	0.0	6.939341137316377E-5
9	0.0	0.0	0.0	0.0	1.3878682274632754E-4
10	0.0	0.0	0.0	0.0	1.3878682274632754E-4
11	0.0	0.0	0.0	0.0	1.3878682274632754E-4
12	0.0	0.0	0.0	6.939341137316377E-5	2.775736454926551E-4
13	0.0	0.0	0.0	6.939341137316377E-5	4.163604682389826E-4
14	0.0	0.0	0.0	6.939341137316377E-5	4.163604682389826E-4
15	0.0	0.0	0.0	1.3878682274632754E-4	6.245407023584738E-4
16	0.0	0.0	0.0	2.081802341194913E-4	6.245407023584738E-4
17	0.0	0.0	0.0	3.469670568658188E-4	6.245407023584738E-4
18	0.0	0.0	0.0	4.163604682389826E-4	6.245407023584738E-4
19	0.0	0.0	0.0	4.8575387961214637E-4	6.245407023584738E-4
20	0.0	0.0	0.0	8.327209364779652E-4	7.633275251048014E-4
21	0.0	0.0	0.0	9.02114347851129E-4	7.633275251048014E-4
22	0.0	0.0	0.0	0.001179687993343784	7.633275251048014E-4
23	6.939341137316377E-5	0.0	0.0	0.0015266550502096028	7.633275251048014E-4
24	6.939341137316377E-5	0.0	0.0	0.0018736221070754216	7.633275251048014E-4
25	6.939341137316377E-5	0.0	0.0	0.0022205891639412406	7.633275251048014E-4
26	6.939341137316377E-5	0.0	0.0	0.002914523277672878	8.327209364779652E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGAT	40	0.0044823624	20.00128	11
ACGATTA	40	0.0044850637	19.999197	32
ATCGTTT	240	0.0	18.665918	29
TCTAGCG	330	0.0	18.423504	28
CTAGCGG	335	0.0	18.148527	29
CATCGTT	260	0.0	17.845438	28
CGTCGTA	280	0.0	17.71542	10
TCGCGTA	145	3.6379788E-12	17.657528	9
CGTCTTA	120	1.6607373E-9	17.332638	15
GTATTAG	370	0.0	16.432913	1
AACCGCG	225	0.0	16.357738	7
TACCGTC	315	0.0	16.256136	7
GCGCAAG	435	0.0	16.184381	1
ACCGTCG	310	0.0	16.002134	8
CGCGTAA	160	1.8189894E-11	16.001024	10
GTCTAGG	160	1.8189894E-11	16.00047	1
CAAGACG	450	0.0	16.00047	4
TAGCGGC	380	0.0	15.999357	30
TAGCGTA	335	0.0	15.763298	7
CGCAAGA	470	0.0	15.318535	2
>>END_MODULE