FastQCFastQC Report
Thu 2 Feb 2017
SRR4062892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388301
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT70651.8194647966397204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT48501.2490310352020726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26250.6760219520423589No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA25710.6621152147432018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.5753268727095733No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA14950.3850105974488863No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11050.28457305028830726No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA10970.28251279291065434No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA9170.23615700191346403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8320.21426676727590194No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA7640.1967545795658523No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.19031627526068692No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG6590.16971370148415793No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA5880.1514289172574884No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA5720.14730840250218258No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA5630.14499061295232307No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT5240.13494685823626518No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG4420.1138292201153229No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA4320.11125389839325678No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT4240.10919364101560386No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.10610325494912452No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT4040.1040429975714716No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC4000.10301286888264516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAG200.003751884231.99755531
GACCGTT302.2436549E-531.9975537
TGCACTG200.003754251231.9934335
GGTATAG308.334888E-426.7058981
TATTAGG308.366339E-426.6886862
AGATTAG308.410535E-426.6646298
CCGTTAT308.410535E-426.6646299
CTACTAG401.5751127E-424.0353091
TGAACTT401.591728E-423.99816710
TACGGCC401.591728E-423.99816719
GTAGCGA601.7583989E-723.9981659
GGTAAGT552.2137156E-623.27094828
TCGGGTA552.2137156E-623.27094825
AAGTTCC552.2137156E-623.27094831
TAAGGTA552.2161948E-623.267955
TCGCCCC350.002065009722.85539425
GAACTTA350.002065009722.85539411
CCTAAGG854.274625E-1022.5923273
TAGTACT502.7930988E-522.3982894
GCATGGG653.8058533E-722.1521539
Produced by FastQC (version 0.11.5)